The invention disclose a strain with high destruxins yield and application thereof. Classification status of the strain MaQ10 is as follows: asexual generation eumycophyta, mitospore eumycophyta, hyphomycetes, moniliales, hyphomycetaceae and metarrhizium; the strain is conserved at China Center for Type Culture Collection, conservation date is October 27, 2008, the conservation number is CCTCC M 208173. The metarrhizium strain has the advantages of high yield of destruxins A and destruxins B, stable strain yield, in particular good stability of destruxins yield before the 10th generation. Thestrain can provide technical base for increasing the actual yield and reducing production cost of the destruxins.