The invention relates to a culture apparatus for liquid strain in the edible fungi, which comprises a glass bottle (1), a rubber plug (2), a glass pipe (3), a double-bulb cavity glass bulb pipe (4) and a connecting hose (5). The culture apparatus is characterized in that the diameter of the big head of the rubber plug (2) is slightly more than that of the glass bottle cap, two holes are punched in the rubber plug (2), and the glass pipe (3) and the double-bulb cavity glass bulb pipe (4) are inserted in the holes; the glass pipe (3) is an air inlet pipe, the length of the glass pipe (3) is slightly higher than the height of the glass bottle, the double-bulb cavity glass bulb pipe (4) is connected to the upper end of the glass pipe (3) through the silicone rubber pipe (5), the lower end of the glass pipe (3) penetrates through the rubber plug, and the distance between the port and the bottle bottom is 1-2 cm; and the double-bulb cavity glass bulb pipe (4) is filled with clean cotton so as to be convenient for filtering the air. The invention can produce the liquid strain in the edible fungi instead of equipment such as fermentation tanks, oscillating table, and the like, has small investment and low production cost, and can save lots of electric power resources and running cost. In addition, the apparatus is made of material easy to obtain, is low in cost, rapid in installation and simple and convenient for operation, thereby being popularized and applied in wide rural households.