The invention relates to a recombinant chicken Alpha-interferon injection and a preparation method, belonging to the technical field of animal medicine production. The recombinant chicken Alpha-interferon injection is characterized by consisting of the following components with the weight percentage: 1 to 5 percent of astragalus polyose, 0.01 to 0.05 percent of phenylformic acid, 5 to 20 percent of original liquid of chicken Alpha-interferon, 0.1 to 0.5 percent of albumin and the rest is injection water. The injection water is a solvent; and the size of a single interferon product is 10ml. The product can be used for saving the medicine cost during the culturing process by 10 to 35 percent; moreover, the product has very good effects on diarrhea and enteritis caused by chicken rotavirus; simultaneously the death rate caused by the descending of resistance caused by immunological suppression during the chickling period can be reduced.