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发明授权
CN101386825B 一种产果胶酶的工程菌株 失效 - 权利终止

一种产果胶酶的工程菌株
摘要：
一种产果胶酶的工程菌株，它涉及一种产酶工程菌株。本发明提供了一种用于微生物发酵的产果胶酶的工程菌株。产果胶酶的工程菌株大肠埃希氏菌HDDMG05属于埃希氏菌属，保藏于中国典型培养物保藏中心，保藏号为CCTCC No：M 208082；大肠埃希氏菌HDDMG05为革兰氏阴性菌，无芽孢，菌株个体呈短杆状，长为0.2～0.7μm，宽为0.2～0.5μm；大肠埃希氏菌HDDMG05菌落呈圆形，白色，隆起，边缘呈波浪状，表面光滑有光泽，透明，菌落挑起软。可采用LB液体培养基发酵本发明大肠埃希氏菌HDDMG05，其发酵条件与LB液体培养基培养大肠杆菌的相同；发酵液中大肠埃希氏菌HDDMG05菌数浓度为1×107～1×108cfu/mL时发酵液中果胶酶的酶活力达8200U/mL以上。
摘要（英）：

The invention relates to an engineering strain for producing pectinase, which relates to an engineering strain for producing enzyme. The invention provides an engineering strain for producing the pectinase used for microbial fermentation. The engineering strain for producing the pectinase, namely Colon bacillus HDDMG05 belongs to Bacillus, and is preserved in the China Center for Type Culture Collection, with a preserving number of CCTCC No: M 208082. The Colon bacillus HDDMG05 belongs to Gram-positive bacteria and has no spore; an individual strain is of a short bar shape, the length of the individual strain is between 0.2 and 0.7 micrometer and the width of the individual strain is between 0.2 and 0.5 micrometer; and a colony of the Colon bacillus HDDMG05 is circular, ivory-white and ridged, has wavy edge and smooth and glossy surface, is transparent, and is soft when lifted. The Colon bacillus HDDMG05 can be fermented by an LB liquid culture medium, and the fermentation condition of the Colon bacillus HDDMG05 is the same as that of culture of Escherichia coli by the LB liquid culture medium; and the enzyme activity of the pectinase in fermentation broth reaches more than 8,200U/mL when the microbial content concentration of the Colon bacillus HDDMG05 in the fermentation broth is between 1x10<7> and 1x10<8> cfu/mL.

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