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发明授权
CN101186629B 一种利用RNAi培育抗CMV和TMV植物的方法及其专用双链RNA 失效 - 权利终止

一种利用RNAi培育抗CMV和TMV植物的方法及其专用双链RNA
摘要：
本发明公开了一种利用RNAi培育抗CMV和TMV植物的方法及其专用双链RNA。该双链RNA，是由正义链和反义链组成的双链RNA；所述正义链的序列是序列表中序列3，或在严格条件下可与序列表中序列3限定的RNA序列杂交的核苷酸序列；所述反义链的核苷酸序列是序列表中序列4，或在严格条件下可与序列表中序列4限定的RNA序列杂交的核苷酸序列。该培育抗CMV和TMV植物的方法，是将含有上述双链RNA编码基因的重组表达载体导入植物中，得到抗CMV和TMV的植物。该双链RNA可降解目的RNA，提高植物抗CMV和TMV的能力。通过RNA干扰来增强植物的抗病性，避免了其mRNA的翻译，不产生病毒蛋白，避免了安全性方面的问题。
摘要（英）：

The invention discloses a method for cultivating plant resisting CMV and TMV via RNAi, and a special double-stranded RNA, wherein the double-stranded RNA is composed of a sense strand and an anti-sense strand, the sequence of the sense strand is represented as sequence 3 in sequence table or nucleic acid sequence which can be crossed with RNA sequence limited in the sequence 3 of sequence table in strict conditions, the nucleic acid sequence of the anti-sense strand is represented as sequence 4 of sequence table or the nucleic acid sequence which can be crossed with RNA sequence limited in the sequence 4 of sequence table in strict conditions. The inventive method leads recombination expression carrier with the double-stranded RNA coding gene into plant to obtain plant resisting CMV and TMV. The double-stranded RNA can degrade target RNA, and improve the CMV-TMV resistant ability of plant. The invention improves disease resistance of plant via RNA interference, which eliminates the translation of mRNA, eliminates virus protein and eliminates safety problem.

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