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CN101052725A T1R异寡聚味觉受体、表达所述受体的细胞系和味觉化合物 无效 - 驳回 复审申请

  • Patent Title: T1R异寡聚味觉受体、表达所述受体的细胞系和味觉化合物
  • Patent Title (English): T1R hetero-oligomeric taste receptors, cell lines that express said receptors, and taste compounds
  • Application No.: CN200480022612.5
    Application Date: 2004-08-06
  • Publication No.: CN101052725A
    Publication Date: 2007-10-10
  • Inventor: 李晓东 , 莉娜·斯塔谢夫斯基 , 徐红
  • Applicant: 西诺米克斯公司
  • Applicant Address: 美国加利福尼亚州
  • Assignee: 西诺米克斯公司
  • Current Assignee: 西诺米克斯公司
  • Current Assignee Address: 美国加利福尼亚州
  • Agency: 北京三友知识产权代理有限公司
  • Agent 丁香兰
  • Priority: 60/494,071 2003.08.06 US; 60/552,064 2004.03.09 US
  • International Application: PCT/US2004/025459 2004.08.06
  • International Announcement: WO2005/015158 EN 2005.02.17
  • Date entered country: 2006-02-06
  • Main IPC: C12P21/06
  • IPC: C12P21/06
T1R异寡聚味觉受体、表达所述受体的细胞系和味觉化合物
Abstract:
本发明涉及特异性结合T1R1/T1R3或T1R2/T1R3受体或其片段或亚基的化合物。本发明还涉及包含T1R1/T1R3和T1R2/T1R3的异寡聚和嵌合的味觉受体在鉴定分别对鲜味刺激物和甜味刺激物产生应答的化合物的检测中的用途。此外，本发明还涉及在组成性或诱导性条件下稳定或瞬时共表达T1R1和T1R3，或T1R2和T1R3的组合的细胞系的构成。本发明还提供所述细胞系在基于细胞的测定中用以鉴定鲜味和甜味的调节化合物的用途，所述测定尤其是采用荧光测定成像法检测受体活性的高通量筛选测定。
Abstract(English):

The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or subunits thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.

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