An efficient method of xylanase immobilization and improving reutilization effect thereof, the immobilization method is: blending Eudragit L-100 and xylanase by a certain proportion, agitating continuously 1.5-3 hours at 25 DEG C to obtain xylanase. After reaction of the xylanase and substrate, adding coagulator, regulating pH value from 3.8 to 4.2 by acetic acid, quiescence 25-40 minutes, centrifugating 30 minutes in condition of 5000xg to finish recovery of immobilized enzyme. The advantages of the invention used to industrialized enzyme method for producing xyloolgosaccharides are: efficient immobilized efficiency for immobilizing enzyme on the carriers; the immobilized enzyme can homogeneous react with substrate to improve enzyme efficiency; using method of improving reutilization of the immobilized enzyme can improve reutilization efficiency of immobilized xylanase using reversible solution material Eudragit L-100 remarkbly as carrier.