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公开(公告)号：US20170233723A1
公开(公告)日：2017-08-17
申请号：US15451716
申请日：2017-03-07
Applicant: University of Ottawa
Inventor： Maxim V. Berezovski , Mohamed Wehbe , Mahmoud Aziz Mahmoud Labib , Darija Muharemagic , Anna S. Zamay , Shahrokh Ghobadloo
IPC: C12N15/10 , C12N15/115
CPC classification number: C12N15/1048 , C07K1/14 , C12N7/00 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12N2760/20051 , C12N2760/20251
Abstract: The disclosure relates to a switchable aptamer having a high affinity for a selected target such as a virus, cell or antibody when in the presence of a binding ion and a low affinity for said target in the absence of said binding ion. The switchable aptamer may be isolated or selected from a pool comprising a mixture of aptamers by incubating the pool with the target ligand and a binding ion to form target-aptamer complexes; separating unbound aptamer molecules from the target-aptamer complexes; contacting the target-aptamer complexes with a chelating agent having affinity for the binding ion wherein a switchable aptamer specific to said target is released from the target-aptamer complexes; and isolating the switchable aptamer released in the preceding step.
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公开(公告)号：US20140342918A1
公开(公告)日：2014-11-20
申请号：US14277110
申请日：2014-05-14
Applicant: Maxim V. Berezovski , Mohamed Wehbe , Mahmoud Aziz Mahmoud Labib , Darija Muharemagic , Anna S. Zamay , Shahrokh Ghobadloo
Inventor： Maxim V. Berezovski , Mohamed Wehbe , Mahmoud Aziz Mahmoud Labib , Darija Muharemagic , Anna S. Zamay , Shahrokh Ghobadloo
IPC: C12N15/10 , C12N7/00 , C07K1/14 , C12N15/115
CPC classification number: C12N15/1048 , C07K1/14 , C12N7/00 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12N2760/20051 , C12N2760/20251
Abstract: The disclosure relates to a switchable aptamer having a high affinity for a selected target such as a virus, cell or antibody when in the presence of a binding ion and a low affinity for said target in the absence of said binding ion. The switchable aptamer may be isolated from a pool comprising a mixture of aptamers by incubating the pool with the target ligand and a binding ion to form target-aptamer complexes; separating unbound aptamer molecules from the target-aptamer complexes; contacting the target-aptamer complexes with a chelating agent having affinity for the binding ion wherein a switchable aptamer specific to said target is released from the target-aptamer complexes; and isolating the switchable aptamer released in the preceding step.
Abstract translation: 本公开内容涉及当在存在结合离子的情况下对所选择的靶，例如病毒，细胞或抗体具有高亲和力的可切换适配体，并且在不存在所述结合离子时对所述靶具有低亲和力。 可以通过将池与靶配体孵育而与包含适配子的混合物的池分离出可切换的适配体和结合离子以形成靶 - 适配子复合物; 从靶 - 适配子复合物分离未结合的适体分子; 使目标适体复合物与对结合离子具有亲和力的螯合剂接触，其中所述靶特异性的可切换适配体从靶 - 适体复合物释放; 并隔离前述步骤中释放的可切换适配体。
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公开(公告)号：US20120134963A1
公开(公告)日：2012-05-31
申请号：US13382355
申请日：2010-07-07
Applicant: John Bell , Jean-Simon Diallo
Inventor： John Bell , Jean-Simon Diallo
CPC classification number: A61K35/766 , A61K31/275 , A61K31/341 , A61K31/365 , A61K38/00 , A61K45/06 , C07C49/675 , C07C49/747 , C07C205/37 , C07C217/84 , C07C233/32 , C07C233/38 , C07C243/38 , C07C255/37 , C07C311/44 , C07C323/42 , C07C323/60 , C07C335/16 , C07C337/06 , C07C337/08 , C07C2601/02 , C07C2601/14 , C07C2603/26 , C07C2603/34 , C07D213/75 , C07D233/90 , C07D257/04 , C07D307/58 , C07D319/20 , C07D333/04 , C07D403/12 , C07D409/12 , C12N7/00 , C12N2760/20232 , C12N2760/20251 , A61K2300/00
Abstract: Provided are viral sensitizing compounds that enhance the efficacy of viruses by increasing spread of the virus in cells, increasing the titer of virus in cells, or increasing the cytotoxicity of virus to cells. Other uses, compositions and methods of using same are also provided.
Abstract translation: 提供的病毒敏化化合物通过增加病毒在细胞中的扩散，增加病毒在细胞中的滴度或增加病毒对细胞的细胞毒性而增强病毒的功效。 还提供了其他用途，组合物和使用方法。
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4.发明申请Purification processes for isolating purified vesicular stomatitis virus from cell culture 有权从细胞培养物中分离纯化的水泡性口炎病毒的纯化方法
公开(公告)号：US20070249019A1
公开(公告)日：2007-10-25
申请号：US11788071
申请日：2007-04-19
Applicant: Yun Kang , Mark William Cutler , Amadou Affrey Ouattara , Kristen Elissa Syvertsen
Inventor： Yun Kang , Mark William Cutler , Amadou Affrey Ouattara , Kristen Elissa Syvertsen
CPC classification number: C12N7/00 , A61K39/00 , A61K48/00 , A61K2039/5252 , A61K2039/5254 , A61K2039/5256 , C07K14/005 , C12N15/86 , C12N2740/16222 , C12N2760/20243 , C12N2760/20251 , C12N2760/20252 , Y02A50/464
Abstract: Novel purification processes for obtaining vesicular stomatitis virus (VSV) of improved purity from mammalian cell culture are described herein. More particularly, in certain embodiments, a process is described for purifying VSV from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 μm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 μm filter and recovering the VSV in the filtered solution.
Abstract translation: 本文描述了从哺乳动物细胞培养物获得改善纯度的水泡性口炎病毒（VSV）的新型纯化方法。 更具体地，在某些实施方案中，描述了用于从感染VSV的哺乳动物细胞培养物的细胞培养液中纯化VSV的方法，该方法包括：通过低速离心澄清细胞培养液并回收上清液中的VSV; 通过0.2至0.45 mum过滤器过滤上清液并回收过滤溶液中的VSV; 将VSV过滤的溶液加载到用第一pH缓冲盐溶液平衡的阴离子交换膜吸附器上，用第二pH缓冲盐溶液从阴离子交换膜吸附器洗脱VSV并回收洗脱的VSV级分; 通过切向流过滤（TFF）使用分子量截留值在300kDa至1,000kDa之间的TFF膜来纯化回收的VSV，并回收渗余物中的VSV，并通过0.2至0.22mum的过滤器过滤VSV滞留物并回收VSV 在过滤的溶液中。
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公开(公告)号：US20070111309A1
公开(公告)日：2007-05-17
申请号：US11540055
申请日：2006-09-29
Applicant: Marcelo Daelli
Inventor： Marcelo Daelli
IPC: C12N5/06
CPC classification number: A61K39/12 , A61K39/13 , A61K39/245 , A61K2039/525 , C12N5/0686 , C12N7/00 , C12N2500/95 , C12N2510/02 , C12N2710/16634 , C12N2710/16651 , C12N2760/20251 , C12N2770/32634 , C12N2770/32651 , Y02A50/386 , Y02A50/466
Abstract: A Vero cell line that can be grown in serum-free and protein-free culture and in suspension culture in the absence of supporting materials for its adherence, a process to obtain said Vero cell line and a process for producing viral vaccines with said cell line, a virus obtainable by said process and vaccines formulated by said virus.
Abstract translation: Vero细胞系，其可在无血清和无蛋白质培养物和悬浮培养物中生长，不存在用于其粘附的支持材料，获得所述Vero细胞系的方法和用所述细胞系生产病毒疫苗的方法 ，由所述方法可获得的病毒和由所述病毒配制的疫苗。
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公开(公告)号：US20240307469A1
公开(公告)日：2024-09-19
申请号：US18680187
申请日：2024-05-31
Applicant: Ottawa Hospital Research Institute
Inventor： Jean-Simon Diallo , Mohammed Selman , Rozanne Arulanandam , Nicole Elise Forbes , Ramya Krishnan
IPC: A61K35/766 , A61K31/225 , A61K39/00 , A61P35/00 , C07C69/60 , C12N7/00
CPC classification number: A61K35/766 , A61K31/225 , A61P35/00 , C07C69/60 , C12N7/00 , A61K2039/585 , C12N2710/10332 , C12N2710/10351 , C12N2740/16134 , C12N2760/16134 , C12N2760/16151 , C12N2760/20232 , C12N2760/20251
Abstract: Provided herein are fumaric-containing compounds, compositions comprising the same and methods for using such compounds for increasing the capacity of an interferon-sensitive virus to infect, transduce, grow or replicate in a cell or spread within one or more tissues. Also provided are methods for increasing the capacity of an interferon-sensitive virus to produce a virus-encoded transgene from a cell, by administering the compounds and compositions.
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公开(公告)号：US20230183650A1
公开(公告)日：2023-06-15
申请号：US17811363
申请日：2022-07-08
Applicant: Boehringer Ingelheim International GmbH
Inventor： Tobias Nolden
CPC classification number: C12N5/0686 , C12N15/86 , C12N2760/20251 , C12N2760/20241
Abstract: The present invention relates to a method for rescue of Vesicular Stomatitis Virus (VSV) from DNA in a HEK293 cell line or a HEK293 cell line adapted to suspension growth comprising (a) providing cells from a HEK293 cell line or a HEK293 cell line adapted to suspension growth in cell culture, (b) transfecting the cells with at least one plasmid, wherein the at least one plasmid comprises (i) an expression cassette comprising a VSV genomic cDNA; (ii) at least one expression cassette encoding VSV nucleoprotein (N) protein, VSV phosphoprotein (P) protein, and VSV large (L) protein; and (iii) an expression cassette encoding SV40 Large T antigen; (c) culturing the transfected cells; and (d) harvesting the cell culture supernatant comprising the rescued VSV. Also provided is the use of a HEK293 cell line or a HEK293 cell line adapted to suspension growth for rescue of Vesicular Stomatitis Virus (VSV) or the use of a plasmid encoding SV40 Large T antigen for rescue of Vesicular Stomatitis Virus (VSV) in a HEK293 cell line or a HEK293 cell line adapted to suspension growth HEK293-F cells by means of transient transfection.
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公开(公告)号：US20180237753A1
公开(公告)日：2018-08-23
申请号：US15919554
申请日：2018-03-13
Applicant: TURNSTONE LIMITED PARTNERSHIP
Inventor： Carolina Solange ILKOW , Fabrice LE BOEUF , John Cameron BELL , Jean-Simon DIALLO , Rozanne ARULANANDAM
IPC: C12N7/00 , A61K35/768 , A61K35/766 , C07K14/50 , A61K38/17 , A61K38/18 , A61K35/76
CPC classification number: C12N7/00 , A61K35/76 , A61K35/766 , A61K35/768 , A61K38/1793 , A61K38/1825 , C07K14/50 , C07K2319/02 , C12N2710/16651 , C12N2710/24151 , C12N2720/12051 , C12N2760/16151 , C12N2760/16152 , C12N2760/18451 , C12N2760/18452 , C12N2760/20232 , C12N2760/20251 , Y02A50/385 , Y02A50/393 , Y02A50/465 , A61K2300/00
Abstract: Described herein is a method of enhancing virus replication in permissive cells that express a receptor to FGF2 protein. The method includes administering FGF2 protein or a functional variant thereof and the virus to the permissive cells. An oncolytic virus having a genome that includes an open reading frame that encodes FGF2 protein or a functional variant thereof is also described.
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公开(公告)号：US20150202240A1
公开(公告)日：2015-07-23
申请号：US14569416
申请日：2014-12-12
Applicant: Ottawa Hospital Research Institute
Inventor： John Bell , Jean-Simon Diallo , Fabrice Le Boeuf
IPC: A61K35/766 , A61K31/275 , C12N7/00 , A61K31/365
CPC classification number: A61K35/766 , A61K31/275 , A61K31/341 , A61K31/365 , A61K38/00 , A61K45/06 , C07C49/675 , C07C49/747 , C07C205/37 , C07C217/84 , C07C233/32 , C07C233/38 , C07C243/38 , C07C255/37 , C07C311/44 , C07C323/42 , C07C323/60 , C07C335/16 , C07C337/06 , C07C337/08 , C07C2601/02 , C07C2601/14 , C07C2603/26 , C07C2603/34 , C07D213/75 , C07D233/90 , C07D257/04 , C07D307/58 , C07D319/20 , C07D333/04 , C07D403/12 , C07D409/12 , C12N7/00 , C12N2760/20232 , C12N2760/20251 , A61K2300/00
Abstract: Provided are viral sensitizing compounds that enhance the efficacy of viruses by increasing spread of the virus in cells, increasing the titer of virus in cells, or increasing the cytotoxicity of virus to cells. Other uses, compositions and methods of using same are also provided.
Abstract translation: 提供的病毒敏化化合物通过增加病毒在细胞中的扩散，增加病毒在细胞中的滴度或增加病毒对细胞的细胞毒性而增强病毒的功效。 还提供了其他用途，组合物和使用方法。
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10.发明授权Purification processes for isolating purified vesicular stomatitis virus from cell culture 有权从细胞培养物中分离纯化的水泡性口炎病毒的纯化方法
公开(公告)号：US07875446B2
公开(公告)日：2011-01-25
申请号：US11788071
申请日：2007-04-19
Applicant: Yun Kang , Mark William Cutler , Amadou Affrey Ouattara , Kristen Elissa Syvertsen
Inventor： Yun Kang , Mark William Cutler , Amadou Affrey Ouattara , Kristen Elissa Syvertsen
CPC classification number: C12N7/00 , A61K39/00 , A61K48/00 , A61K2039/5252 , A61K2039/5254 , A61K2039/5256 , C07K14/005 , C12N15/86 , C12N2740/16222 , C12N2760/20243 , C12N2760/20251 , C12N2760/20252 , Y02A50/464
Abstract: A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 μm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 μm filter and recovering the VSV in the filtered solution.
Abstract translation: 描述了从感染VSV的哺乳动物细胞培养物的细胞培养液中纯化水泡性口炎病毒（VSV）的方法，该方法包括：通过低速离心澄清细胞培养液并回收上清液中的VSV; 通过0.2至0.45μm的过滤器过滤上清液并在过滤的溶液中回收VSV; 将VSV过滤的溶液加载到用第一pH缓冲盐溶液平衡的阴离子交换膜吸附器上，用第二pH缓冲盐溶液从阴离子交换膜吸附器洗脱VSV并回收洗脱的VSV级分; 通过切向流过滤（TFF），使用分子量截止值在300kDa至1,000kDa之间的TFF膜来纯化回收的VSV，并回收渗余物中的VSV，并通过0.2至0.22μm的过滤器过滤VSV滞留物并回收VSV 在过滤的溶液中。
