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公开(公告)号：US20240393342A1
公开(公告)日：2024-11-28
申请号：US18695747
申请日：2022-09-27
Applicant: The University of Tokyo , RIKEN
Inventor： Sadao OTA , Ken WATANABE , Fumiko KAWASAKI , Yuka MORI
Abstract: A cell labeling molecule comprising a particle 101 having an identifiable property, an identification sequence 102 that is identifiable and corresponds to the property of the particle 101, a cleavable linker 103 that binds the particle 101 and the identification sequence 102, and a binding molecule 104 for binding to a cell, the binding molecule being bound to the identification sequence 102.
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公开(公告)号：US20230058670A1
公开(公告)日：2023-02-23
申请号：US17770569
申请日：2021-01-12
Applicant: ThinkCyte, Inc. , The University of Tokyo , RIKEN
Inventor： Asako TSUBOUCHI , Sadao OTA , Fumiko KAWASAKI
IPC: C12N15/10
Abstract: The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.
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公开(公告)号：US20230348893A1
公开(公告)日：2023-11-02
申请号：US18186118
申请日：2023-03-17
Applicant: ThinkCyte, Inc. , The University of Tokyo , RIKEN
Inventor： Asako TSUBOUCHI , Sadao OTA , Fumiko KAWASAKI
IPC: C12N15/10
CPC classification number: C12N15/1055 , C12N15/1065 , C12N15/1089 , C40B30/04
Abstract: The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.
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公开(公告)号：US20220010373A1
公开(公告)日：2022-01-13
申请号：US17291739
申请日：2019-11-07
Applicant: THE UNIVERSITY OF TOKYO , RIKEN
Inventor： Nozomu YACHIE , Sadao OTA , Fumiko KAWASAKI
IPC: C12Q1/6876 , C12Q1/686
Abstract: The present invention provides a method for detecting genome-related information of a cell coexisting with at least one type of test substance, for various types of test substances. More specifically, the present invention provides a method for detecting genome-related information of a cell or a derivative thereof coexisting with at least one type of test substance, including:
preparing a plurality of types of first compartments that contain at least one type of test substance and a first barcode nucleic acid, and a plurality of types of second compartments that contain a cell or a derivative thereof and a second barcode nucleic acid-linked bead;
forming a plurality of types of third compartments in which one first compartment and one second compartment are fused;
hybridizing each of the genome-related nucleic acid and the first barcode nucleic acid with a second barcode nucleic acid to obtain a hybridized complex;
producing an amplified product derived from the hybridized complex; and
detecting genome-related information in the cell after coexistence with the test substance using an expression pattern of the amplified product as an index.
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公开(公告)号：US20180246030A1
公开(公告)日：2018-08-30
申请号：US15552438
申请日：2016-02-24
Applicant: The University of Tokyo , Osaka University
Inventor： Sadao OTA , Ryoichi HORISAKI , Kazuki HASHIMOTO
Abstract: Any one or both of an optical system with a structured lighting pattern and a structured detecting system having a plurality of regions with different optical characteristics are used. In addition, optical signals from an object to be observed through one or a small number of pixel detectors are detected while changing relative positions between the object to be observed and any one of the optical system and the detecting system, time series signal information of the optical signals are obtained, and an image associated with an object to be observed from the time series signal information is reconstructed.
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公开(公告)号：US20230175949A1
公开(公告)日：2023-06-08
申请号：US17924135
申请日：2021-09-10
Applicant: The University of Tokyo
Inventor： Sadao OTA , Masashi UGAWA
IPC: G01N15/14
CPC classification number: G01N15/1434 , G01N15/1404 , G01N2015/1443 , G01N2015/142
Abstract: An imaging flow cytometer includes: a flow channel in which an observation object flows and a length in a width direction is longer than a length in a height direction; an acoustic element configured to apply acoustic waves as standing waves to the flow channel; a light source that irradiates the flow channel with illumination light; an image sensor configured to image at least a line included in a cross section of the observation object crossing a flow line direction which is a direction in which the observation object flows in the flow channel by measuring or imaging the observation object passing through a position irradiated with the illumination light; and circuitry configured to generate an image in which the observation object is scanned in the flow line direction on the basis of a plurality of captured images acquired by the imaging unit imaging the line in a time series.
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公开(公告)号：US20200150022A1
公开(公告)日：2020-05-14
申请号：US16610481
申请日：2018-05-02
Applicant: THINKCYTE, INC. , The University of Tokyo
Inventor： Masashi UGAWA , Yoko KAWAMURA , Sadao OTA
IPC: G01N15/14 , G01N33/483 , C12Q1/02 , G01N15/10
Abstract: A cell evaluation system includes physical measurement unit, a database, and evaluation unit. The evaluation unit refers to a relevance stored in the database, searches reference measurement information based on measurement information of a cell newly measured via the physical measurement unit, and evaluates the cell with biological measurement information associated with the searched reference measurement information.
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公开(公告)号：US20200027020A1
公开(公告)日：2020-01-23
申请号：US16584535
申请日：2019-09-26
Applicant: ThinkCyte, Inc. , The University of Tokyo
Inventor： Ryosuke KAMESAWA , Sadao OTA
IPC: G06N20/00
Abstract: A learning result output apparatus includes a machine learning unit that performs machine learning on at least one of attributes of a learning target, with a degree of the attribute as an evaluation axis, on the basis of morphological information indicating a shape of the learning target, and a graph information generation unit that generates graph information indicating a graph representing a learning result obtained by performing machine learning in the machine learning unit, with the evaluation axis as an axis, on the basis of a learning model indicating the learning result.
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公开(公告)号：US20210269844A1
公开(公告)日：2021-09-02
申请号：US16610227
申请日：2018-05-02
Applicant: The University of Tokyo
Inventor： Sadao OTA
Abstract: The present invention provides a method for simple and effective monitoring of a dynamic change in a cell or a derivative thereof. More specifically, the present invention provides a method for monitoring a dynamic change in a cell, which includes preparing a plurality of compartments containing at least one cell or a derivative thereof and at least one bead, and repeatedly obtaining over time both state measurement information of the cell or the derivative thereof in each compartment, and imaging information of the bead to monitor a dynamic change in each cell or derivative thereof in the plurality of compartments, wherein the imaging information of the bead in each compartment can be clearly distinguished from each other and becomes an index of identifying the cell or the derivative thereof contained in each compartment.
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公开(公告)号：US20200057289A1
公开(公告)日：2020-02-20
申请号：US16663182
申请日：2019-10-24
Applicant: ThinkCyte, Inc. , The University of Tokyo
Inventor： Masashi UGAWA , Yoko KAWAMURA , Sadao OTA
Abstract: An imaging flow cytometer includes at least one flow channel through which an observation target flows, a light source which irradiates the flow channel with sheet-like excitation light, an imaging unit which images a specific cross-section of the observation target by imaging fluorescence from the observation target having passed through a position irradiated with the excitation light, and a three-dimensional image generation unit which generates a three-dimensional image of the observation target as a captured image on the basis of a plurality of captured images obtained by cross-sectional imaging by the imaging unit.
