公开(公告)号：US20230381296A1

公开(公告)日：2023-11-30

申请号：US18031570

申请日：2021-10-13

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： Shinya OKAMURA ,  Akiho KASHIWABARA ,  Hirotaka EBINA

IPC: A61K39/215 ,  C12N7/00 ,  C12N9/10 ,  C12N9/16 ,  A61P31/14

CPC classification number: A61K39/215 ,  C12N7/00 ,  C12N9/1007 ,  C12Y201/01056 ,  C12Y201/01057 ,  C12N9/16 ,  C12Y301/03084 ,  A61P31/14 ,  C12N2770/20022 ,  C12N2770/20034 ,  C12N2770/20071 ,  A61K2039/5254

Abstract: Provided is a strain that is effective as an active ingredient of a vaccine against betacoronavirus. This SARS-CoV-2 includes non-structural protein(s) that has the following responsible mutation(s): a mutation in the amino acid residue corresponding to the L of position 445 of SEQ ID NO: 1 in NSP3; a mutation in the amino acid residues corresponding to the G of position 248 and the G of position 416 of SEQ ID NO: 2 in NSP14; and/or a mutation in the amino acid residue corresponding to the V of position 67 of SEQ ID NO: 3 in NSP16.

公开(公告)号：US20250002871A1

公开(公告)日：2025-01-02

申请号：US18708189

申请日：2022-11-07

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： Shiro TAKEKAWA ,  Hirotaka EBINA ,  Shinya OKAMURA ,  Akiho YOSHIDA

IPC: C12N7/00 ,  A61K9/00 ,  A61K39/00 ,  A61P31/14 ,  A61P37/04

Abstract: The purpose of the present invention is to provide a strain that is useful as a novel betacoronavirus vaccine. It is revealed that novel betacoronavirus, according to the present invention, having a prescribed substitution mutation relating to temperature sensitivity in combination with a prescribed deletion mutation relating to attenuation, is useful as a betacoronavirus vaccine strain having excellent attenuated characteristics.

公开(公告)号：US20230374469A1

公开(公告)日：2023-11-23

申请号：US18031599

申请日：2021-10-13

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： Shinya OKAMURA ,  Akiho KASHIWABARA ,  Hirotaka EBINA

IPC: C12N7/00 ,  C07K14/165 ,  A61P31/14

CPC classification number: C12N7/00 ,  C07K14/165 ,  A61P31/14 ,  A61K2039/5254

Abstract: Strains that is effective as the active component of a vaccine against the betacoronavirus is provided. A SARS-CoV-2 containing structural protein(s) and/or non-structural protein(s) having the following mutation(s): the amino acid residue mutations in NSP3, corresponding to V at position 404, L at position 445, K at position 1792 and/or D at position 1832 in SEQ ID No. 1; the amino acid residue mutations in NSP14, corresponding to G at position 248, G at position 416, and/or A at position 504 in SEQ ID No. 2; the amino acid residue mutation in NSP16, corresponding to V at position 67 in SEQ ID No. 3; the amino acid residue mutations in the spike, corresponding to L at position 54, T at position 739 and/or A at position 879 in SEQ ID No. 4; the amino acid residue mutation in the envelope, corresponding to L at position 28 in SEQ ID No. 5; and/or, the amino acid residue mutation in the nucleocapsid, corresponding to S at position 2 in SEQ ID No. 6;