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公开(公告)号:US20230331790A1
公开(公告)日:2023-10-19
申请号:US17913749
申请日:2021-03-24
Inventor: Hiroshi TOMITA , Eriko SUGANO
IPC: C07K14/405 , A61P27/02 , C12N15/63
CPC classification number: C07K14/405 , A61P27/02 , C12N15/63 , C07K2319/03
Abstract: An object of the present invention is to provide a modified channelrhodopsin having high ion permeability (photoreactivity). The solution is to substitute the third extracellular domain counted from the N-terminal side of three extracellular domains included in a Volvox carteri-derived channelrhodopsin by a corresponding extracellular domain of a Chlamydomonas reinhardtii-derived channelrhodopsin-2.
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公开(公告)号:US20240270799A1
公开(公告)日:2024-08-15
申请号:US17913769
申请日:2021-03-24
Inventor: Hiroshi TOMITA , Eriko SUGANO
IPC: C07K14/405 , A61K38/00 , A61P27/02 , C12N15/86
CPC classification number: C07K14/405 , A61P27/02 , C12N15/86 , A61K38/00 , C07K2319/03 , C12N2750/14143
Abstract: An object of the present invention is to provide a modified photoreceptive chloride channel having excellent photoreactive properties in which the wavelength sensitivity range is narrow, and also both τon and τoff are short. The solution is a polypeptide obtained by substituting a region from the fourth transmembrane domain to the sixth transmembrane domain counted from the N-terminal side of a Guillardia theta-derived photoreceptive chloride channel-1 (GtACR1) by a corresponding region of a Guillardia theta-derived photoreceptive chloride channel-2 (GtACR2).
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公开(公告)号:US20210347831A1
公开(公告)日:2021-11-11
申请号:US17277468
申请日:2019-09-15
Inventor: Hiroshi TOMITA , Eriko SUGANO , Kitako TABATA , Yoshito WATANABE
IPC: C07K14/405 , C12N15/86 , A61P27/02
Abstract: An object of the present invention is to provide a modified channel rhodopsin capable of opening and closing an ion channel by irradiation with light at different wavelengths, and/or having high ion permeability (photoreactivity). The solution is to substitute a C-terminal region of a channel rhodopsin obtained by substituting an N-terminal region of a Volvox carteri-derived channel rhodopsin by an N-terminal region of a Chlamydomonas reinhardtii-derived channel rhodopsin-1, by a C-terminal region of a Chlamydomonas reinhardtii-derived channel rhodopsin-2 or a C-terminal region of a Tetraselmis striata-derived channel rhodopsin.