公开(公告)号：US20130085072A1

公开(公告)日：2013-04-04

申请号：US13631331

申请日：2012-09-28

Applicant: LOS ALAMOS NATIONAL SECURITY, LLC

Inventor： Andrew M. Bradbury ,  Csaba Kiss ,  Sara D'Angelo ,  Fortunato Ferrara ,  Leslie A. Naranjo ,  Tiziano Gaiotto

IPC: C40B30/04 ,  C40B40/10 ,  C07K16/00 ,  C40B10/00

CPC classification number: G01N33/6854 ,  C07K16/005 ,  C07K16/1289 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C12Q1/02 ,  G01N33/6845

Abstract: Described herein are methods that combine phage and yeast display to create polyclonal antibodies that are renewable, and when amplified over 100 million fold, maintain diversity without loss of representation of any of the antibodies present. The antibody representation remains essentially constant, as confirmed by deep sequencing. The provided methods allow generation, use and propagation of polyclonal antibodies, without concern that representation is lost. Furthermore, because the derivation of the polyclonal pool is carried out in vitro using phage and yeast display, it is possible in various embodiments to eliminate reactivities that are considered undesirable. Additionally, the polyclonal pool can be enriched for higher affinity antibodies.

Abstract translation: 本文描述了将噬菌体和酵母显示组合以产生可再生的多克隆抗体的方法，并且当扩增超过1亿倍时，保​​持多样性而不损失任何存在的抗体。 通过深度测序证实，抗体表现基本上保持恒定。 所提供的方法允许产生，使用和繁殖多克隆抗体，而不用担心丢失表示。 此外，由于多克隆池的衍生是使用噬菌体和酵母显示在体外进行的，所以在各种实施方案中可以消除被认为是不合需要的反应性。 此外，多克隆池可以富集以获得更高亲和力的抗体。