公开(公告)号：US20190177787A1

公开(公告)日：2019-06-13

申请号：US16206957

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  Alfred LUI ,  Guobing XIANG ,  Synne LARSEN

IPC: C12Q1/6874 ,  C08J7/14 ,  C12Q1/6853 ,  C12Q1/6876

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

公开(公告)号：US20170009275A1

公开(公告)日：2017-01-12

申请号：US15202487

申请日：2016-07-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. MENCHEN ,  Guobing XIANG ,  Prasanna Krishnan THWAR ,  Jeffrey FREDIANI ,  Allen WONG ,  Alfred LUI ,  Lily LU

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2525/117 ,  C12Q2525/197 ,  C12Q2565/549 ,  C12Q2563/149

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

Abstract translation: 水凝胶网络包括具有偶联位点的水凝胶聚合物，在末端与偶联位点处的水凝胶聚合物共轭的寡核苷酸和耦合在寡核苷酸的末端与偶联位点之间的功能部分。 这样的水凝胶网络可以通过以下方法形成：包括活化底物的偶联位点并将与寡核苷酸的末端连接的连接体结合到活化的偶联位点，连接在寡核苷酸的末端和 接头部分。