公开(公告)号：US11015220B2

公开(公告)日：2021-05-25

申请号：US16123166

申请日：2018-09-06

Applicant: Life Technologies Corporation

Inventor： Theo Nikiforov ,  Daniel Mazur ,  Xinzhan Peng ,  Tommie Lloyd Lincecum ,  Yuri Belosludtsev ,  Howard Reese ,  Dmitriy Gremyachinskiy ,  Roman Rozhkov ,  John Mauro ,  Joseph Beechem ,  Eric Tulsky ,  Imad Naasani ,  Kari Haley ,  Joseph Treadway

IPC: C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

公开(公告)号：US09365838B2

公开(公告)日：2016-06-14

申请号：US14087307

申请日：2013-11-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo Nikiforov ,  Daniel Mazur ,  Xinzhan Peng ,  Tommie Lloyd Lincecum ,  Yuri Belosludtsev ,  Howard Reese ,  Dmitriy Gremyachinskiy ,  Roman Rozhkov ,  John Mauro ,  Joseph Beechem ,  Eric Tulsky ,  Imad Naasani ,  Kari Haley ,  Joseph Treadway

IPC: C12N9/12 ,  C07H19/20 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

公开(公告)号：US20140206096A1

公开(公告)日：2014-07-24

申请号：US14170349

申请日：2014-01-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph BARTEL ,  Yongfen Chen ,  Eric Tulsky ,  Joseph Treadway

IPC: G01N21/64 ,  H01L33/06 ,  H01L21/02

CPC classification number: G01N21/6486 ,  B82Y5/00 ,  B82Y15/00 ,  C01B25/082 ,  C09K11/02 ,  C09K11/565 ,  C09K11/70 ,  C30B7/00 ,  C30B29/40 ,  C30B29/48 ,  C30B29/60 ,  G01N33/588 ,  H01L21/02392 ,  H01L21/02557 ,  H01L21/02601 ,  H01L21/02628 ,  H01L33/06

Abstract: Nanocrystals having an indium-based core and methods for making them and using them to construct core-shell nanocrystals are described. These core-shell nanocrystals are highly stable and provide higher quantum yields than known nanocrystals of similar composition, and they provide special advantages for certain applications because of their small size.

Abstract translation: 描述了具有铟基核的纳米晶体及其制备方法和用于构建核壳纳米晶体的方法。 这些核 - 壳纳米晶体是高度稳定的并且提供比已知具有类似组成的纳米晶体更高的量子产率，并且由于它们的小尺寸，它们为某些应用提供了特殊的优点。

公开(公告)号：US20180158907A1

公开(公告)日：2018-06-07

申请号：US15867684

申请日：2018-01-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric WELCH ,  Joseph Bartel ,  Eric Tulsky ,  Joseph Treadway ,  Yongfen Chen

IPC: H01L29/06 ,  H01L33/04 ,  H01L31/0352 ,  G01N33/58 ,  B82Y40/00 ,  B82Y30/00 ,  B82Y20/00

CPC classification number: H01L29/0665 ,  B82Y20/00 ,  B82Y30/00 ,  B82Y40/00 ,  C30B7/00 ,  C30B29/40 ,  C30B29/48 ,  C30B29/60 ,  G01N33/533 ,  G01N33/588 ,  H01L21/02628 ,  H01L31/035209 ,  H01L33/04 ,  H01L51/0007

Abstract: A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.

公开(公告)号：US09476885B2

公开(公告)日：2016-10-25

申请号：US14590840

申请日：2015-01-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric Tulsky ,  Kari Haley ,  Imad Naasani ,  John Mauro ,  Roman Rozhkov ,  Joseph Treadway

IPC: G01N33/58 ,  G01N1/30 ,  G01N21/64

CPC classification number: G01N33/588 ,  B01J13/14 ,  B22F1/0022 ,  B22F1/02 ,  B82Y30/00 ,  G01N1/30 ,  G01N21/64 ,  G01N2001/302 ,  Y10S977/773 ,  Y10S977/81 ,  Y10S977/834 ,  Y10S977/902

Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

公开(公告)号：US09263638B2

公开(公告)日：2016-02-16

申请号：US14073078

申请日：2013-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marc D. Schrier ,  Donald Zehnder ,  Joseph Treadway ,  Joseph Bartel

IPC: H01L33/28 ,  B82Y30/00 ,  C09C1/10 ,  C09K11/02 ,  C09K11/56 ,  C09K11/88

CPC classification number: H01L33/28 ,  B82Y30/00 ,  C01P2004/04 ,  C01P2004/64 ,  C09C1/10 ,  C09K11/02 ,  C09K11/565 ,  C09K11/883 ,  Y10S977/824 ,  Y10S977/834 ,  Y10T428/2991

Abstract: A method is provided for preparing luminescent semiconductor nanoparticles composed of a first component X, a second component A, and a third component B, wherein X, A, and B are different, by combining B with X and A in an amount such that the molar ratio B:(A+B) is in the range of approximately 0.001 to 0.20 and the molar ratio X:(A+B) is in the range of approximately 0.5:1.0 to 2:1. The characteristics of the thus-prepared nanoparticles can be substantially similar to those of nanoparticles containing only X and B while maintaining many useful properties characteristic of nanoparticles containing only X and A. The nanoparticles so prepared can additionally exhibit emergent properties such as a peak emission energy less than that characteristic of a particle composed of XA or XB alone; this method is particularly applicable to the preparation of stable, bright nanoparticles that emit in the red to infrared regions of the electromagnetic spectrum. Luminescent semiconductor nanoparticles having exemplary properties are also provided.

Abstract translation: 提供一种制备由第一组分X，第二组分A和第三组分B组成的发光半导体纳米颗粒的方法，其中X，A和B不同，通​​过将B与X和A组合使得其量使得 摩尔比B：（A + B）在约0.001至0.20的范围内，摩尔比X：（A + B）在约0.5:1.0至2:1的范围内。 如此制备的纳米颗粒的特征可以与仅含有X和B的纳米颗粒的特征基本相似，同时保持了仅含有X和A的纳米颗粒的许多有用特性。如此制备的纳米颗粒可另外显示出出现特性，例如峰值发射能 小于单独由XA或XB组成的颗粒的特征; 该方法特别适用于在电磁光谱的红色至红外区域中发射的稳定的明亮纳米粒子的制备。 还提供了具有示例性质的发光半导体纳米颗粒。

公开(公告)号：US10084042B2

公开(公告)日：2018-09-25

申请号：US15867684

申请日：2018-01-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric Welch ,  Joseph Bartel ,  Eric Tulsky ,  Joseph Treadway ,  Yongfen Chen

IPC: H01L29/06 ,  H01L33/04 ,  H01L31/0352 ,  G01N33/58 ,  H01L21/02 ,  H01L51/00 ,  G01N33/533 ,  B82Y30/00 ,  C30B29/60 ,  C30B29/48 ,  C30B29/40 ,  C30B7/00 ,  B82Y20/00 ,  B82Y40/00

CPC classification number: H01L29/0665 ,  B82Y20/00 ,  B82Y30/00 ,  B82Y40/00 ,  C30B7/00 ,  C30B29/40 ,  C30B29/48 ,  C30B29/60 ,  G01N33/533 ,  G01N33/588 ,  H01L21/02628 ,  H01L31/035209 ,  H01L33/04 ,  H01L51/0007

Abstract: A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.

公开(公告)号：US10686034B2

公开(公告)日：2020-06-16

申请号：US16107953

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric Welch ,  Joseph Bartel ,  Eric Tulsky ,  Joseph Treadway ,  Yongfen Chen

IPC: H01L29/06 ,  B82Y30/00 ,  C30B7/00 ,  C30B29/40 ,  C30B29/48 ,  C30B29/60 ,  G01N33/533 ,  H01L51/00 ,  H01L21/02 ,  G01N33/58 ,  H01L31/0352 ,  H01L33/04 ,  B82Y40/00 ,  B82Y20/00

Abstract: A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.

公开(公告)号：US10093972B2

公开(公告)日：2018-10-09

申请号：US15166372

申请日：2016-05-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Theo Nikiforov ,  Daniel Mazur ,  Xinzhan Peng ,  Tommie Lloyd Lincecum ,  Yuri Belosludtsev ,  Howard Reese ,  Dmitriy Gremyachinskiy ,  Roman Rozhkov ,  John Mauro ,  Joseph Beechem ,  Eric Tulsky ,  Imad Naasani ,  Kari Haley ,  Joseph Treadway

IPC: C07K14/00 ,  C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

公开(公告)号：US20170186840A1

公开(公告)日：2017-06-29

申请号：US15401216

申请日：2017-01-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric WELCH ,  Joseph Bartel ,  Eric Tulsky ,  Joseph Treadway ,  Yongfen Chen

IPC: H01L29/06 ,  H01L33/04 ,  G01N33/58 ,  H01L31/0352

CPC classification number: H01L29/0665 ,  B82Y20/00 ,  B82Y30/00 ,  B82Y40/00 ,  C30B7/00 ,  C30B29/40 ,  C30B29/48 ,  C30B29/60 ,  G01N33/533 ,  G01N33/588 ,  H01L21/02628 ,  H01L31/035209 ,  H01L33/04 ,  H01L51/0007

Abstract: A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.