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公开(公告)号：US11845973B2
公开(公告)日：2023-12-19
申请号：US17679007
申请日：2022-02-23
Applicant: LG CHEM, LTD.
Inventor： Hye Ok Kang , Donggyun Kang , Chul Woong Kim , In Young Huh , Jung Yun Choi
IPC: C08G63/06 , C12P7/625 , C12N1/20 , C12N15/70 , C12N9/04 , C12N9/02 , C12N9/10 , C08G63/08 , C12N9/88
CPC classification number: C12P7/625 , C08G63/06 , C08G63/08 , C12N1/20 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/13 , C12N9/88 , C12N15/70 , C12Y101/01027 , C12Y101/01028 , C12Y102/01003 , C12Y203/01 , C12Y208/03001 , C12Y402/0103 , C12Y402/01036 , C12N2510/02
Abstract: The present invention relates to a novel 3-hydroxypropionate-lactate block copolymer [P(3HP-b-LA)], and a method for preparing same, and more specifically, provides a method for preparing a 3-hydroxypropionate-lactate block copolymer, and a 3-hydroxypropionate-lactate block copolymer produced thereby, the method comprising: a first culture step in which, by using recombinant E. coli improved so as to be incapable of biosynthesizing lactic acid, P(3HP) is biosynthesized at the early stage of culturing by having glycerol as a carbon source and through 3-hydroxypropionate-generating genes and an enhanced PHA synthase; and a second culture step in which P(3HP) production is inhibited by using a carbon catabolic repression system for selectively introducing only glucose into E. coli when glycerol and glucose are supplied together as carbon sources, and in which polylactate is biosynthesized to an interrupted P(3HP) terminus by the enabling of the expression of a lactate synthase and a lactyl-CoA converting enzyme through an IPTG induction system.
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公开(公告)号：US11359057B2
公开(公告)日：2022-06-14
申请号：US16615625
申请日：2018-12-31
Applicant: LG CHEM, LTD.
Inventor： Jung Yun Choi , Hye Ok Kang , Donggyun Kang , Younghyun Cho , Jae Hyung Kim , Chul Woong Kim , In Young Huh
Abstract: Provided is a lactic acid-based block copolymer which is biodegradable while having excellent mechanical properties.
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公开(公告)号：US11773223B2
公开(公告)日：2023-10-03
申请号：US17733698
申请日：2022-04-29
Applicant: LG CHEM, LTD.
Inventor： Jung Yun Choi , Hye Ok Kang , Donggyun Kang , Younghyun Cho , Jae Hyung Kim , Chul Woong Kim , In Young Huh
CPC classification number: C08G81/027 , C08G18/281 , C08G18/606 , C08G18/73
Abstract: Provided is a lactic acid-based block copolymer which is biodegradable while having excellent mechanical properties.
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公开(公告)号：US11286510B2
公开(公告)日：2022-03-29
申请号：US16754480
申请日：2019-03-13
Applicant: LG CHEM, LTD.
Inventor： Hye Ok Kang , Donggyun Kang , Chul Woong Kim , In Young Huh , Jung Yun Choi
IPC: C12P7/62 , C08G63/06 , C12N1/21 , C12N15/70 , C12N9/04 , C12N9/02 , C12N9/10 , C12N9/88 , C12P7/625 , C12N1/20 , C08G63/08
Abstract: Provided are a novel 3-hydroxypropionate-lactate block copolymer [P(3HP-b-LA)], and a method for preparing same, comprising: a) transforming a recombinant microorganism modified to be incapable of biosynthesizing lactic acid with a vector including a 3-hydroxypropionyl-CoA biosynthesis gene and a polyhydroxyalkanoate (PHA) synthetase gene, and a vector including a lactate biosynthesis gene and a gene of an enzyme that converts lactate to lactyl-CoA; (b) synthesizing poly(3-hydroxypropionate) (P(3HP)) by culturing the recombinant microorganism using a glycerol as a carbon source; and (c) inhibiting P(3HP) production by adding IPTG and glucose, and biosynthesizing polylactate (PLA) at the end of P(3HP) synthesized in step (b) by enabling the expression of a lactate biosynthesis enzyme and an enzyme that converts lactate to lactyl-CoA. Also provided is a recombinant microorganism produced in step a).
