公开(公告)号：US20200088743A1

公开(公告)日：2020-03-19

申请号：US16685432

申请日：2019-11-15

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo-Hyun LEE ,  Ji-Yoon KANG ,  Min-Ho KIM ,  Yun-Kyung KIM

IPC: G01N33/68 ,  G01N33/543 ,  G01N27/02 ,  G01N21/64

Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.

公开(公告)号：US20200040190A1

公开(公告)日：2020-02-06

申请号：US16403041

申请日：2019-05-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jun-Seok LEE ,  Dhiraj P. MURALE ,  Seong-Cheol HONG ,  Kyung-Tae HONG ,  Yun-Kyung KIM ,  Seok LEE

IPC: C09B23/04 ,  G01N21/64

Abstract: Disclosed is a fluorescent probe specifically labeling mitochondria, which can exhibit high transmittance by virtue of light emission in the NIR range and in which nonspecific fluorescence absorption in biomolecules can be avoided, making it possible to observe fluorescence images in deep tissue.

公开(公告)号：US20180217164A1

公开(公告)日：2018-08-02

申请号：US15696302

申请日：2017-09-06

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Soo-Hyun LEE ,  Ji-Yoon KANG ,  Min-Ho KIM ,  Yun-Kyung KIM

IPC: G01N33/68 ,  G01N27/02 ,  G01N21/64 ,  G01N21/76

Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.