公开(公告)号：US20140335545A1

公开(公告)日：2014-11-13

申请号：US14310758

申请日：2014-06-20

Applicant: KOREA BASIC SCIENCE INSTITUTE

Inventor： Zee-Won LEE ,  Soohyun Kim ,  Seung II Kim ,  Jung Me Hwang

IPC: G01N33/50 ,  G01N33/58

CPC classification number: G01N33/5035 ,  C12N15/1055 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/90666 ,  G01N2333/912 ,  G01N2500/02 ,  G01N2500/10

Abstract: A method for detecting the interactions of biomaterials by screening a prey that interacts with a bait includes preparing a cell which expresses a first construct including a translocation module, a first labeling material, and a first medium, and a third construct including a prey and a second labeling material; introducing a second construct into the prepared cell, the second construct including a bait and a second medium binding with the first medium; allowing the prey and the bait to interact each other; and confirming the interaction between the prey and the bait by detecting intracellular distributions of the first construct and the third construct.

Abstract translation: 通过筛选与诱饵相互作用的猎物来检测生物材料的相互作用的方法包括制备表达包含易位模块，第一标记材料和第一培养基的第一构建体的细胞，以及包括猎物和第 第二标签材料; 将第二构建体引入所制备的细胞中，所述第二构建体包含诱饵和与所述第一培养基结合的第二培养基; 允许猎物和诱饵互相交流; 并通过检测第一构建体和第三构建体的细胞内分布来确认猎物和诱饵之间的相互作用。