-
公开(公告)号：US10222349B2
公开(公告)日：2019-03-05
申请号：US15656307
申请日：2017-07-21
Applicant: Intelligent BioSystems, Inc.
Inventor： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
IPC: G01N27/447 , B01L3/00 , C12Q1/68 , C12Q1/6869
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
-
公开(公告)号：US20140234832A1
公开(公告)日：2014-08-21
申请号：US14012622
申请日：2013-08-28
Applicant: INTELLIGENT BIOSYSTEMS, INC.
Inventor： Jerzy Olejnik , Evan Guggenheim , Visalakshi Visalakshi
IPC: C12Q1/68
CPC classification number: C12Q1/6869 , C12Q1/6806 , C12Q1/6825 , G06F19/20 , C12Q2527/137 , C12Q2525/186 , C12Q2525/117 , C12Q2565/601 , C12Q2563/107 , C12Q2537/165
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable Media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
Abstract translation: 本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中的核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。 本发明的方法包括校正核苷酸测序期间遇到的一种或多种现象，例如通过合成方法进行测序。 这些现象包括但不限于序列引导，序列滞后，光谱串扰以及由照明和/或滤波器响应变化引起的噪声。
-
公开(公告)号：US10273539B2
公开(公告)日：2019-04-30
申请号：US15343279
申请日：2016-11-04
Applicant: Intelligent BioSystems, Inc.
Inventor： Mong Sano Marma , Jerzy Olejnik
IPC: C07H21/00 , C12Q1/6869 , C07F7/18 , C07H19/10 , C07H19/14 , C09B1/00 , C09B5/24 , C09B23/16 , C12Q1/6876 , C07C323/60 , C07D207/46 , C12Q1/6811 , C07C323/12 , C09B11/24 , C09B23/08
Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
-
公开(公告)号：US09791409B2
公开(公告)日：2017-10-17
申请号：US14708675
申请日：2015-05-11
Applicant: Intelligent BioSystems, Inc.
Inventor： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
IPC: G01N27/447 , C12Q1/68 , B01L3/00
CPC classification number: G01N27/44791 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/0877 , B01L2400/0487 , C12Q1/6869 , G01N27/44726
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
-
公开(公告)号：US20170137878A1
公开(公告)日：2017-05-18
申请号：US15343279
申请日：2016-11-04
Applicant: Intelligent BioSystems, Inc.
Inventor： Mong Sano Marma , Jerzy Olejnik , IIia Korboukh
IPC: C12Q1/68
CPC classification number: C12Q1/6869 , C07C323/12 , C07C323/60 , C07C2603/18 , C07D207/46 , C07F7/1804 , C07H19/10 , C07H19/14 , C09B1/00 , C09B5/2436 , C09B11/24 , C09B23/083 , C09B23/166 , C12Q1/6811 , C12Q1/6876 , Y02P20/55
Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
-
公开(公告)号：US09434989B2
公开(公告)日：2016-09-06
申请号：US14691042
申请日：2015-04-20
Applicant: Intelligent BioSystems Inc.
Inventor： Steven Gordon , Jerzy Olejnik
CPC classification number: C07H19/00 , C12Q1/6869 , C12Q1/6874 , C12Q2535/101 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N2021/0346 , G01N2021/058 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6471 , G01N2201/062 , G01N2201/0627 , G01N2201/12
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
-
公开(公告)号：US20150299783A1
公开(公告)日：2015-10-22
申请号：US14691042
申请日：2015-04-20
Applicant: Intelligent BioSystems Inc.
Inventor： Steven Gordon , Jerzy Olejnik
IPC: C12Q1/68
CPC classification number: C07H19/00 , C12Q1/6869 , C12Q1/6874 , C12Q2535/101 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N2021/0346 , G01N2021/058 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6471 , G01N2201/062 , G01N2201/0627 , G01N2201/12
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
Abstract translation: 本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。 本发明的方法包括校正核苷酸测序期间遇到的一种或多种现象，例如通过合成方法进行测序。 这些现象包括但不限于序列引导，序列滞后，光谱串扰以及由照明和/或滤波器响应变化引起的噪声。
-
公开(公告)号：US09145589B2
公开(公告)日：2015-09-29
申请号：US14463211
申请日：2014-08-19
Applicant: Intelligent BioSystems, Inc.
Inventor： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
CPC classification number: G01N27/44791 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/0877 , B01L2400/0487 , C12Q1/6869 , G01N27/44726
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
Abstract translation: 本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。 为了提供更大的灵活性和效率，采用多个较小的流动池，每个具有相对小的要被成像的面积。
-
公开(公告)号：US20130301888A1
公开(公告)日：2013-11-14
申请号：US13857547
申请日：2013-04-05
Applicant: Intelligent Biosystems, Inc.
Inventor： Steven Gordon , Phillip A. Veatch , Jerzy Olejnik
IPC: G06F19/20
CPC classification number: C12Q1/6869 , C12Q1/6806 , C12Q1/6825 , G06F19/20 , C12Q2527/137 , C12Q2525/186 , C12Q2525/117 , C12Q2565/601 , C12Q2563/107 , C12Q2537/165
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
Abstract translation: 本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。 本发明的方法包括校正核苷酸测序期间遇到的一种或多种现象，例如通过合成方法进行测序。 这些现象包括但不限于序列引导，序列滞后，光谱串扰以及由照明和/或滤波器响应变化引起的噪声。
-
公开(公告)号：US20180208774A1
公开(公告)日：2018-07-26
申请号：US15928533
申请日：2018-03-22
Applicant: Intelligent Biosystems, Inc.
Inventor： Mong Sano Marma , Jerzy Olejnik , Ilia Korboukh
CPC classification number: C07H19/10 , C07C323/12 , C07C323/60 , C07C2603/18 , C07D207/46 , C07F7/1804 , C07H19/14 , C09B1/00 , C09B5/2436 , C09B11/24 , C09B23/083 , C09B23/166 , C12Q1/6811 , C12Q1/6869 , C12Q1/6876 , Y02P20/55
Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
