公开(公告)号：US07169394B2

公开(公告)日：2007-01-30

申请号：US11318983

申请日：2005-12-27

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K39/295

CPC classification number: A61K9/0024 ,  A61K39/0241 ,  A61K39/12 ,  A61K39/145 ,  A61K39/39 ,  A61K47/06 ,  A61K47/10 ,  A61K47/32 ,  A61K2039/521 ,  A61K2039/525 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16134 ,  Y02A50/48

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably CARBOPOL®, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polyoxypropylene block copolymer such as PLURONIC®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA. In another embodiment, the invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration, and comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant or adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity, in combination with other vaccine components.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选的是CARBOPOL，以及可代谢的油如一种或多种不饱和萜烯烃，优选角鲨烯或角鲨烷的混合物，以及聚氧乙烯 - 聚氧丙烯嵌段共聚物 作为PLURONIC（R）。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。 在另一个实施方案中，本发明提供了改进的猪肺炎支原体疫苗组合物，其有利地在单次施用后提供免疫感染，并且包含灭活的猪肺炎支原体菌苗和佐剂或佐剂混合物，其组合提供猪肺炎支原体 单次给药后感染，并引起特异于猪肺炎支原体菌的免疫应答，并与其他疫苗组分结合使用，包括细胞介导的免疫和局部（分泌型IgA）免疫。

公开(公告)号：US09056909B2

公开(公告)日：2015-06-16

申请号：US12265274

申请日：2008-11-05

Applicant: Hsien-Jue Chu ,  Zhichang Xu ,  Wumin Li ,  Nicole Rae Gibson

Inventor： Hsien-Jue Chu ,  Zhichang Xu ,  Wumin Li ,  Nicole Rae Gibson

IPC: A61K39/02 ,  C12N1/02 ,  C07K14/30 ,  A61K39/116 ,  A61K39/00

CPC classification number: C07K14/30 ,  A61K39/0241 ,  A61K39/116 ,  A61K2039/522 ,  A61K2039/55566 ,  A61K2039/70

Abstract: Provided are immunogenic and vaccine compositions and methods for their preparation and use, which compositions are effective in protecting against, minimizing the severity of, preventing, and/or ameliorating M. hyopneumoniae infection. Administration to an animal of one or two doses of an adjuvanted live avirulent M. hyopneumoniae composition disclosed herein is effective in providing immunity to the animal and protection from infection with a virulent strain of M. hyopneumoniae thereby reducing the severity of and/or preventing disease caused by one or more virulent strain of M. hyopneumoniae. Also provided are compositions, which further comprise one or more antigen such as, for example, one or more live bacteria, bacterin, toxoid, and/or virus and/or viral antigen. Exemplified are immunogenic compositions, comprising an adjuvanted live avirulent M. hyopneumoniae and compositions, comprising Porcine Circovirus Type 1-Type 2 chimera modified live vaccine (cPCV1-2) in further combination with an adjuvanted live avirulent M. hyopneumoniae.

Abstract translation: 提供免疫原性和疫苗组合物及其制备和使用的方法，该组合物有效地防止，最小化预防和/或改善猪肺炎支原体感染的严重性。 向一种或两种剂量的动物给药一种或两种剂量的本文公开的佐剂化的无毒无毒性猪肺炎支原体组合物有效地提供对动物的免疫并且防止感染猪肺炎支原体毒性菌株，从而降低疾病的严重性和/或预防疾病 由一种或多种毒性猪肺炎支原体毒株引起。 还提供了还包含一种或多种抗原例如一种或多种活细菌，菌苗，类毒素和/或病毒和/或病毒抗原的组合物。 示例是免疫原性组合物，其包含佐剂化的无毒力无毒环境的猪肺炎支原体和包含猪佐氮病毒1型2嵌合体修饰的活疫苗（cPCV1-2）的进一步组合的组合物，其与佐剂化的无毒力无毒性猪肺炎支原体组合。

公开(公告)号：US20100119471A1

公开(公告)日：2010-05-13

申请号：US12692275

申请日：2010-01-22

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K31/765 ,  A61P31/04 ,  A61P37/04

CPC classification number: A61K39/0241 ,  A61K2039/521 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  Y10S424/825 ,  A61K2300/00

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably CARBOPOL®, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polyoxypropylene block copolymer such as PLURONIC®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选的是CARBOPOL，以及可代谢的油如一种或多种不饱和萜烯烃，优选角鲨烯或角鲨烷和聚氧乙烯 - 聚氧丙烯嵌段共聚物如PLURONIC ®。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。

公开(公告)号：US20060228373A1

公开(公告)日：2006-10-12

申请号：US11318983

申请日：2005-12-27

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K39/295 ,  A61K9/14

CPC classification number: A61K9/0024 ,  A61K39/0241 ,  A61K39/12 ,  A61K39/145 ,  A61K39/39 ,  A61K47/06 ,  A61K47/10 ,  A61K47/32 ,  A61K2039/521 ,  A61K2039/525 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16134 ,  Y02A50/48

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably Carbopol, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polypropylene block copolymer such as Pluronic®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA. In another emodiment, the invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration, and comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant or adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity, in combination with other vaccine components.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选Carbopol，以及可代谢的油，例如一种或多种不饱和萜烯烃，优选角鲨烯或角鲨烷和聚氧乙烯 - 聚丙烯嵌段共聚物如Pluronic（ R）。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。 另一个实例中，本发明提供了一种改进的猪肺炎支原体疫苗组合物，其有利地在单次给药后提供免疫感染，并且包含灭活的猪肺炎支原体菌苗和佐剂或佐剂混合物，其组合提供了猪肺炎支原体 单次给药后感染，并引起特异于猪肺炎支原体菌的免疫应答，并与其他疫苗组分结合使用，包括细胞介导的免疫和局部（分泌型IgA）免疫。

公开(公告)号：US09238065B2

公开(公告)日：2016-01-19

申请号：US13458541

申请日：2012-04-27

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K39/02 ,  A61K39/00

CPC classification number: A61K39/0241 ,  A61K2039/521 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  Y10S424/825 ,  A61K2300/00

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably CARBOPOL®, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polyoxypropylene block copolymer such as PLURONIC®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选的是CARBOPOL，以及可代谢的油如一种或多种不饱和萜烯烃，优选角鲨烯或角鲨烷和聚氧乙烯 - 聚氧丙烯嵌段共聚物如PLURONIC ®。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。

公开(公告)号：US08187588B2

公开(公告)日：2012-05-29

申请号：US12692275

申请日：2010-01-22

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K39/02 ,  A01N63/00 ,  A61K39/116

CPC classification number: A61K39/0241 ,  A61K2039/521 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  Y10S424/825 ,  A61K2300/00

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably CARBOPOL®, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polyoxypropylene block copolymer such as PLURONIC®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选的是CARBOPOL，以及可代谢的油如一种或多种不饱和萜烯烃，优选角鲨烯或角鲨烷和聚氧乙烯 - 聚氧丙烯嵌段共聚物如PLURONIC ®。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。

公开(公告)号：US20100062018A1

公开(公告)日：2010-03-11

申请号：US12618842

申请日：2009-11-16

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K39/116 ,  A61K39/02 ,  A61P31/04

CPC classification number: A61K9/0024 ,  A61K39/0241 ,  A61K39/12 ,  A61K39/145 ,  A61K39/39 ,  A61K47/06 ,  A61K47/10 ,  A61K47/32 ,  A61K2039/521 ,  A61K2039/525 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16134 ,  Y02A50/48

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably Carbopol, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polypropylene block copolymer such as Pluronic®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA. In another embodiment, the invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration, and comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant or adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity, in combination with other vaccine components.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选Carbopol，以及代谢油如一种或多种不饱和萜烯烃（优选角鲨烯或角鲨烷）和聚氧乙烯 - 聚丙烯嵌段共聚物如Pluronic 。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。 在另一个实施方案中，本发明提供了改进的猪肺炎支原体疫苗组合物，其有利地在单次施用后提供免疫感染，并且包含灭活的猪肺炎支原体菌苗和佐剂或佐剂混合物，其组合提供猪肺炎支原体 单次给药后感染，并引起特异于猪肺炎支原体菌的免疫应答，并与其他疫苗组分结合使用，包括细胞介导的免疫和局部（分泌型IgA）免疫。

公开(公告)号：US07666439B2

公开(公告)日：2010-02-23

申请号：US11507231

申请日：2006-08-21

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K39/02 ,  A61K39/116 ,  A61K39/38 ,  A01N63/00 ,  A01N65/00

CPC classification number: A61K39/0241 ,  A61K2039/521 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  Y10S424/825 ,  A61K2300/00

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably CARBOPOL®, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polyoxypropylene block copolymer such as PLURONIC®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选的是CARBOPOL，以及可代谢的油如一种或多种不饱和萜烯烃，优选角鲨烯或角鲨烷和聚氧乙烯 - 聚氧丙烯嵌段共聚物如PLURONIC ®。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。

公开(公告)号：US20090117152A1

公开(公告)日：2009-05-07

申请号：US12265274

申请日：2008-11-05

Applicant: Hsien-Jue Chu ,  Zhichang Xu ,  Wumin Li ,  Nicole Rae Gibson

Inventor： Hsien-Jue Chu ,  Zhichang Xu ,  Wumin Li ,  Nicole Rae Gibson

IPC: A61K39/02

CPC classification number: C07K14/30 ,  A61K39/0241 ,  A61K39/116 ,  A61K2039/522 ,  A61K2039/55566 ,  A61K2039/70

Abstract: Provided are immunogenic and vaccine compositions and methods for their preparation and use, which compositions are effective in protecting against, minimizing the severity of, preventing, and/or ameliorating M. hyopneumoniae infection. Administration to an animal of one or two doses of an adjuvanted live avirulent M. hyopneumoniae composition disclosed herein is effective in providing immunity to the animal and protection from infection with a virulent strain of M. hyopneumoniae thereby reducing the severity of and/or preventing disease caused by one or more virulent strain of M. hyopneumoniae. Also provided are compositions, which further comprise one or more antigen such as, for example, one or more live bacteria, bacterin, toxoid, and/or virus and/or viral antigen. Exemplified are immunogenic compositions, comprising an adjuvanted live avirulent M. hyopneumoniae and compositions, comprising Porcine Circovirus Type 1-Type 2 chimera modified live vaccine (cPCV1-2) in further combination with an adjuvanted live avirulent M. hyopneumoniae.

Abstract translation: 提供免疫原性和疫苗组合物及其制备和使用的方法，该组合物有效地防止，最小化预防和/或改善猪肺炎支原体感染的严重性。 向一种或两种剂量的动物给药一种或两种剂量的本文公开的佐剂化的无毒无毒性猪肺炎支原体组合物有效地提供对动物的免疫并且防止感染猪肺炎支原体毒性菌株，从而降低疾病的严重性和/或预防疾病 由一种或多种毒性猪肺炎支原体毒株引起。 还提供了还包含一种或多种抗原例如一种或多种活细菌，菌苗，类毒素和/或病毒和/或病毒抗原的组合物。 示例是免疫原性组合物，其包含佐剂化的无毒力无毒环境的猪肺炎支原体和包含猪佐氮病毒1型2嵌合体修饰的活疫苗（cPCV1-2）的进一步组合的组合物，其与佐剂化的无毒力无毒性猪肺炎支原体组合。

公开(公告)号：US20070134269A1

公开(公告)日：2007-06-14

申请号：US11652306

申请日：2007-01-11

Applicant: Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

Inventor： Hsien-Jue Chu ,  Wumin Li ,  Zhichang Xu

IPC: A61K39/295

CPC classification number: A61K9/0024 ,  A61K39/0241 ,  A61K39/12 ,  A61K39/145 ,  A61K39/39 ,  A61K47/06 ,  A61K47/10 ,  A61K47/32 ,  A61K2039/521 ,  A61K2039/525 ,  A61K2039/552 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16134 ,  Y02A50/48

Abstract: The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably Carbopol, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polypropylene block copolymer such as Pluronic®. The vaccine composition may optionally include a preservative, preferably thimerosol and/or EDTA. In another embodiment, the invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration, and comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant or adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity, in combination with other vaccine components.

Abstract translation: 本发明提供了一种改良的猪肺炎支原体疫苗组合物，其在单次给药后有利地提供免疫感染。 组合物包含灭活的猪肺炎支原体疫苗和佐剂混合物，其组合在单次施用后提供对猪肺炎支原体感染的免疫，并引起对猪肺炎支原体菌苗特异性的免疫应答，并且包括细胞介导的免疫和局部（分泌型IgA ）免疫力。 在优选的实施方案中，佐剂混合物包含丙烯酸聚合物，最优选Carbopol，以及可代谢的油，例如一种或多种不饱和萜烯烃，优选角鲨烯或角鲨烷和聚氧乙烯 - 聚丙烯嵌段共聚物如Pluronic（ R）。 疫苗组合物可以任选地包括防腐剂，优选硫柳汞和/或EDTA。 在另一个实施方案中，本发明提供了改进的猪肺炎支原体疫苗组合物，其有利地在单次施用后提供免疫感染，并且包含灭活的猪肺炎支原体菌苗和佐剂或佐剂混合物，其组合提供猪肺炎支原体 单次给药后感染，并引起特异于猪肺炎支原体菌的免疫应答，并与其他疫苗组分结合使用，包括细胞介导的免疫和局部（分泌型IgA）免疫。