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    Method for identifying RNA isoforms in transcriptome using Nanopore RNA reads
    4.
    发明申请
    Method for identifying RNA isoforms in transcriptome using Nanopore RNA reads 有权

    公开(公告)号：US20210139977A1

    公开(公告)日：2021-05-13

    申请号：US17090916

    申请日：2020-11-06

    申请人： Hong Kong Baptist University

    发明人： Zhongying ZHAO ,  Runsheng LI

    IPC分类号： C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6837

    摘要： The present invention provide a method for identifying different isoform using long reads of RNA sequencing. The method includes assigning sequence tracks to a given gene locus based on long-read mapping against a reference genome wherein existing isoforms are also included as a sequence track, excluding long-read mappings that show few overlaps with existing exon or are in antisense to the given gene locus, clustering the sequence tracks based on a distance score Score 1, merging the sequence tracks with a cut-off based on the distance scores Score 1 between the sequence tracks, merging the sequence tracks if the distance score Score 1 is lower than 5%, clustering the retained sequence tracks based on a mutual distance score Score 2, merging the sequence track with a shorter length in the summed exons and correcting the resulting sequence tracks for intron/exon junctions to result in different isoforms.