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    Microbial strains and process for the manufacture of biomaterials
    2.
    发明授权
    Microbial strains and process for the manufacture of biomaterials 有权
    微生物菌株和生物材料制造工艺

    公开(公告)号：US08748139B2

    公开(公告)日：2014-06-10

    申请号：US10607903

    申请日：2003-06-27

    Applicant: Gjalt W. Huisman ,  Laura Z. Luo ,  Oliver P. Peoples

    Inventor： Gjalt W. Huisman ,  Laura Z. Luo ,  Oliver P. Peoples

    IPC: C12P7/52 ,  C12P21/06

    CPC classification number: C12N1/08 ,  C12N9/22 ,  C12P7/625

    Abstract: DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.

    Abstract translation: 提供使用这些DNA构建体构建的DNA构建体和遗传工程化的微生物菌株，其产生对DNA和/或RNA具有特异性的核酸酶。 这些菌株以有效提高聚合物的生产力和/或回收率的量将核酸酶分泌到周质或生长培养基中，并且特别适用于高细胞密度发酵过程。 这些构建体可用于修饰微生物菌株以改善聚合物如细胞内蛋白质如酶，生长因子和细胞因子的生产和回收过程; 用于生产聚羟基链烷酸酯; 并且用于生产细胞外多糖，例如黄原胶，藻酸盐，结冷胶，动物胶，透明质酸和微生物纤维素。

    Microbial strains and processes for the manufacture of biomaterials
    3.
    发明授权
    Microbial strains and processes for the manufacture of biomaterials 有权
    用于制造生物材料的微生物菌株和方法

    公开(公告)号：US08728778B2

    公开(公告)日：2014-05-20

    申请号：US11924350

    申请日：2007-10-25

    Applicant: Gjalt W. Huisman ,  Laura Z. Luo ,  Oliver P. Peoples

    Inventor： Gjalt W. Huisman ,  Laura Z. Luo ,  Oliver P. Peoples

    IPC: C12P7/52 ,  C12P21/06

    CPC classification number: C12N1/08 ,  C12N9/22 ,  C12P7/625

    Abstract: DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.

    Abstract translation: 提供使用这些DNA构建体构建的DNA构建体和遗传工程化的微生物菌株，其产生对DNA和/或RNA具有特异性的核酸酶。 这些菌株以有效提高聚合物的生产力和/或回收率的量将核酸酶分泌到周质或生长培养基中，并且特别适用于高细胞密度发酵过程。 这些构建体可用于修饰微生物菌株以改善聚合物如细胞内蛋白质如酶，生长因子和细胞因子的生产和回收过程; 用于生产聚羟基链烷酸酯; 并且用于生产细胞外多糖，例如黄原胶，藻酸盐，结冷胶，动物胶，透明质酸和微生物纤维素。

    TRANSGENIC SYSTEMS FOR THE MANUFACTURE OF POLY(2-HYDROXY-BUTYRATE-CO-3-HYDROXYHEXANOATE)
    8.
    发明申请
    TRANSGENIC SYSTEMS FOR THE MANUFACTURE OF POLY(2-HYDROXY-BUTYRATE-CO-3-HYDROXYHEXANOATE) 失效
    用于制备聚（2-羟基丁酸酯-3-羟基己酸酯）的转基因系统

    公开(公告)号：US20100311131A1

    公开(公告)日：2010-12-09

    申请号：US12364293

    申请日：2009-02-02

    Applicant: Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    Inventor： Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    IPC: C12P7/62

    CPC classification number: C12N9/88 ,  C12N9/0008 ,  C12P7/625

    Abstract: Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as Escherichia coli or in plant crops as production systems which include PHA biosynthetic genes from PHA producers. In a preferred embodiment of the method, additional genes are introduced in wild type or transgenic polyhydroxybutyrate (PHB) producers, thereby creating new strains that synthesize 3HH monomers which are incorporated into PHAs. The 3HH monomer preferably is derived in microbial systems using butanol or butyrate as feedstocks, which are precursors of 3-hydroxyhexanoyl-CoA. Pathways for in vivo production of butyrol-CoA specifically encompassing butyryl-CoA dehydrogenase activity are provided.

    Abstract translation: 已经开发了合成含有3-羟基己酸作为共聚单体的聚羟基链烷酸酯（PHA）的转基因生物的工程方法。 这些方法基于遗传工程细菌如大肠杆菌或植物作物，作为包括PHA生物合成基因的生产系统。 在该方法的优选实施方案中，在野生型或转基因聚羟基丁酸酯（PHB）生产者中引入另外的基因，从而产生合成并入PHA的3HH单体的新菌株。 3HH单体优选在使用丁醇或丁酸盐作为原料的微生物系统中衍生，其是3-羟基己酰基-CoA的前体。 提供了具体包含丁酰辅酶A脱氢酶活性的丁酰-coA的体内生产途径。

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