公开(公告)号：US09028836B2

公开(公告)日：2015-05-12

申请号：US13568510

申请日：2012-08-07

Applicant: Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

IPC: A61K39/385 ,  A61K39/00 ,  C12N15/113

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: An immunogenic composition containing a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker.

Abstract translation: 含有载体蛋白的聚糖缀合物和包含Globo H，其免疫原性片段或阶段特异性胚胎抗原-4（SSEA-4）的聚糖的免疫原性组合物，其中所述聚糖通过接头与载体蛋白缀合 。

公开(公告)号：US08268969B2

公开(公告)日：2012-09-18

申请号：US12537129

申请日：2009-08-06

Applicant: Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

IPC: C07K16/00 ,  A01N43/04 ,  C07H17/00

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: Immunogenic compositions, cancer vaccines and methods for treating cancer are provided. Compositions comprising: (a) a glycan such as Globo H or an immunogenic fragment thereof, wherein the glycan is conjugated with a carrier protein by a linker such as para-nitrophenyl; and (b) an adjuvant comprising glycolipid capable of binding CD1d on a dendritic cell, such as an α-galactosyl-ceramide derivative, wherein the immunogenic composition induces an immune response that induces a higher relative level of IgG isotype antibodies as compared to IgM isotype antibodies, are provided. Immunogenic compositions comprising the carrier protein diphtheria toxin cross-reacting material 197 (DT-CRM197) and the adjuvant C34 are provided. Antibodies generated by immunogenic compositions disclosed herein further neutralize at least one of the antigens Globo H, stage-specific embryonic antigen-3 (SSEA-3) and stage-specific embryonic antigen-4 (SSEA-4). Therapeutics against breast cancer stem cells comprising immunogenic compositions comprising Globo H, SSEA-3 or SSEA-4 conjugated with DT-CRM197.

Abstract translation: 提供免疫原性组合物，癌症疫苗和治疗癌症的方法。 组合物，其包含：（a）聚糖如Globo H或其免疫原性片段，其中所述聚糖通过诸如对硝基苯基之类的接头与载体蛋白缀合; 和（b）包含能够结合树突状细胞上的CD1d的糖脂如α-半乳糖基 - 神经酰胺衍生物的佐剂，其中所述免疫原性组合物诱导与IgM同种型相比诱导更高相对水平的IgG同种型抗体的免疫应答 抗体。 提供了包含载体蛋白质白喉毒素交叉反应材料197（DT-CRM197）和佐剂C34的免疫原性组合物。 由本文公开的免疫原性组合物产生的抗体进一步中和至少一种抗原Globo H，阶段特异性胚胎抗原-3（SSEA-3）和阶段特异性胚胎抗原-4（SSEA-4）。 包括含有与DT-CRM197缀合的Globo H，SSEA-3或SSEA-4的免疫原性组合物的乳腺癌干细胞的治疗剂。

公开(公告)号：US08158367B2

公开(公告)日：2012-04-17

申请号：US12485516

申请日：2009-06-16

Applicant: Chi-Huey Wong ,  Chung-Yi Wu ,  Cheng-Chi Wang ,  Alice L. Yu

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Cheng-Chi Wang ,  Alice L. Yu

IPC: G01N33/53

CPC classification number: G01N33/57488 ,  G01N33/57415

Abstract: A cancer diagnostic method using a glycan array that contains Gb5 and Globo H, Bb2, Bb3, and/or Bb4.

Abstract translation: 使用含有Gb5和Globo H，Bb2，Bb3和/或Bb4的聚糖阵列的癌症诊断方法。

公开(公告)号：US20110250192A1

公开(公告)日：2011-10-13

申请号：US13056043

申请日：2009-08-03

Applicant: Alice L. Yu ,  You-Chin Lin ,  Ruey-Jen Lin

Inventor： Alice L. Yu ,  You-Chin Lin ,  Ruey-Jen Lin

IPC: A61K38/46 ,  A61K48/00 ,  A61P35/00 ,  C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/178

Abstract: Methods for assessing the risk level or survival/death probability of a neuroblastoma patient based on a number of microRNA signatures, optionally in combination with Dicer, Drosha, and age at diagnosis. Also disclosed herein is use of Dicer, Drosha, or both in suppressing neuroblastoma cell growth

Abstract translation: 基于许多微小RNA标记来评估神经母细胞瘤患者的风险水平或存活/死亡概率的方法，任选地与Dicer，Drosha和诊断年龄组合。 本文还公开了使用Dicer，Drosha或两者来抑制神经母细胞瘤细胞生长

公开(公告)号：US20090317411A1

公开(公告)日：2009-12-24

申请号：US12485546

申请日：2009-06-16

Applicant: Chi-Huey Wong ,  Alice L. Yu ,  John. Yu

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  John. Yu

IPC: A61K39/00 ,  A61K31/7105 ,  A61P35/00

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: An immune composition containing Globo H or its fragment (e.g., SSEA3) and an adjuvant, e.g., α-GalCer, for eliciting immune responses against Globo H or its fragment and uses thereof in cancer treatment. Also disclosed is a method of treating cancer by inhibiting the activity of one of FUT1 and FUT2, both of which involve in Globo H biosynthesis.

Abstract translation: 包含Globo H或其片段（例如SSEA3）和佐剂（例如α-GalCer）的免疫组合物，用于引发针对Globo H或其片段的免疫应答及其在癌症治疗中的用途。 还公开了通过抑制FUT1和FUT2之一的活性来治疗癌症的方法，两者都涉及Globo H生物合成。

公开(公告)号：US09459247B2

公开(公告)日：2016-10-04

申请号：US13075160

申请日：2011-03-29

Applicant: Huan-Chang Lin ,  Hsin-Hung Lin ,  Cai-Yu Kao ,  Alice L Yu ,  Wen-Ping Peng ,  Chung-Hsuan Chen

Inventor： Huan-Chang Lin ,  Hsin-Hung Lin ,  Cai-Yu Kao ,  Alice L Yu ,  Wen-Ping Peng ,  Chung-Hsuan Chen

IPC: A61K45/06 ,  C12Q1/70 ,  G01N33/50 ,  G01N33/569 ,  G01N33/68

CPC classification number: G01N33/5091 ,  G01N33/5008 ,  G01N33/569 ,  G01N33/6848

Abstract: Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.

Abstract translation: 提供了通过细胞质谱法（CMS）检测纳米颗粒或微粒的存在的方法。 提供CMS方法用于确定每个细胞中纳米颗粒或微粒的数量。 通过本发明的CMS方法可以确定其细胞内浓度的纳米颗粒包括聚合物纳米颗粒（NP），脂质体，基于病毒的NP，碳纳米管，金刚石NP，聚合胶束，纳米载体，脂质体和病毒纳米颗粒。 公开了根据本发明的药物递送效率和病原体细胞内滴度的测定。 提供了确定病毒颗粒的细胞内吸收的方法。

公开(公告)号：US20120328646A1

公开(公告)日：2012-12-27

申请号：US13568510

申请日：2012-08-07

Applicant: Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Alice L. Yu ,  John Yu

IPC: A61K39/385 ,  A61P35/00 ,  C07K14/33 ,  C07K14/00 ,  C07K14/34

CPC classification number: A61K39/0011 ,  A61K2039/55511 ,  A61K2039/6037 ,  A61K2039/6081 ,  A61K2039/627 ,  C12N15/1137 ,  C12N2310/14

Abstract: An immunogenic composition containing a glycan conjugate including a carrier protein, and a glycan including Globo H, an immunogenic fragment thereof, or stage-specific embryonic antigen-4 (SSEA-4), wherein the glycan is conjugated with the carrier protein through a linker.

Abstract translation: 含有载体蛋白的聚糖缀合物和包含Globo H，其免疫原性片段或阶段特异性胚胎抗原-4（SSEA-4）的聚糖的免疫原性组合物，其中所述聚糖通过接头与载体蛋白质缀合 。

公开(公告)号：US20110236882A1

公开(公告)日：2011-09-29

申请号：US13075160

申请日：2011-03-29

Applicant: Huan-Chang LIN ,  Hsin-Hung LIN ,  Cai-Yu KAO ,  Alice L. Yu ,  Wen-Ping PENG ,  Chung-Hsuan Chen

Inventor： Huan-Chang LIN ,  Hsin-Hung LIN ,  Cai-Yu KAO ,  Alice L. Yu ,  Wen-Ping PENG ,  Chung-Hsuan Chen

IPC: C12Q1/70 ,  C12Q1/02 ,  C12Q1/68

CPC classification number: G01N33/5091 ,  G01N33/5008 ,  G01N33/569 ,  G01N33/6848

Abstract: Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.

Abstract translation: 提供了通过细胞质谱法（CMS）检测纳米颗粒或微粒的存在的方法。 提供CMS方法用于确定每个细胞中纳米颗粒或微粒的数量。 通过本发明的CMS方法可以确定其细胞内浓度的纳米颗粒包括聚合物纳米颗粒（NP），脂质体，基于病毒的NP，碳纳米管，金刚石NP，聚合胶束，纳米载体，脂质体和病毒纳米颗粒。 公开了根据本发明的药物递送效率和病原体细胞内滴度的测定。 提供了确定病毒颗粒的细胞内吸收的方法。

公开(公告)号：US08486914B2

公开(公告)日：2013-07-16

申请号：US12750655

申请日：2010-03-30

Applicant: Chi-Huey Wong ,  Alice L. Yu ,  Wen-Bin Yang ,  Eugene Fan ,  Hsien-Yeh Hsu ,  Peishan Lee ,  Tseng-Rong Tu ,  Chin-Chung Hung

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Wen-Bin Yang ,  Eugene Fan ,  Hsien-Yeh Hsu ,  Peishan Lee ,  Tseng-Rong Tu ,  Chin-Chung Hung

IPC: A61K31/715

CPC classification number: A61K31/715

Abstract: Compositions comprising Hirsutella sinensis mycelia extracts and chromatographically separated polysaccharide-enriched fractions thereof are provided. Methods for extracting Hirsutella sinensis mycelia are provided. Compositions for methods for their use in amelioration, prevention and treatment of sepsis, acute endotoxemia and inflammatory responses are disclosed.

Abstract translation: 提供了包含中华绒螯蟹菌丝体提取物和色谱分离的多糖富集部分的组合物。 提供了中华绒螯蟹提取物的方法。 公开了其用于改善，预防和治疗败血症，急性内毒素血症和炎性反应的方法的组合物。

公开(公告)号：US5032507A

公开(公告)日：1991-07-16

申请号：US120470

申请日：1987-11-13

Applicant: John Yu ,  Alice L. Yu ,  Joan Vaughan ,  Jean E. F. Rivier ,  Wylie W. Vale, Jr.

Inventor： John Yu ,  Alice L. Yu ,  Joan Vaughan ,  Jean E. F. Rivier ,  Wylie W. Vale, Jr.

IPC: A61K38/00 ,  A61K38/04 ,  A61K38/22 ,  A61P7/00 ,  C07K14/435 ,  C07K14/575

CPC classification number: A61K38/22 ,  Y10S435/81

Abstract: A method for modulating the rate of erythropoiesis in human hematopoietic progenitor cells. FRP is found to be an efficacious potentiator of, and inhibin a suppressor of, erythropoietin-induced differentiation. FRP and inhibin are shown to be functional antagonists of each other, and thus represent an effective means for modulating erythropoiesis in a number of disease states which are directly caused by or associated with an abnormal rate of erythropoiesis.

Abstract translation: 调节人类造血祖细胞中红细胞生成速率的方法。 发现FRP是促红细胞生成素诱导分化的有效增效剂，抑制抑制素。 FRP和抑制素显示为彼此的功能性拮抗剂，因此代表了直接由异常红细胞生成速率引起或与之相关的许多疾病状态调节红细胞生成的有效手段。