公开(公告)号：US08962343B2

公开(公告)日：2015-02-24

申请号：US10506426

申请日：2003-03-05

申请人： Robert Alexander Ventzki ,  Josef Stegemann

发明人： Robert Alexander Ventzki ,  Josef Stegemann

IPC分类号： G01N1/18 ,  G01N27/447 ,  G01N35/00

CPC分类号： G01N27/44769 ,  G01N27/44721 ,  G01N27/44782 ,  G01N35/0099 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

摘要： The invention relates to a process and a device for parallel fractionating a multiplicity of individual samples (29) within a 3-dimensional separation medium (6), with the subsequent process steps being carried out:A first space (2) which extends essentially across all three space coordinates contains the separation medium (6). A multiplicity of individual samples (29) is arranged close to an interface of the first space (2), with the individual samples (29) being arranged essentially in a planar fashion, i.e. their particular center-of-gravity positions are described by two coordinates. Under the influence of one or more physical parameters, the individual samples (29) are able to migrate essentially perpendicularly to the area of their application through the separation medium (6), and are fractionated in the process according to one or more of their properties. Suitable physical parameters are, for example, electric forces (electrophoresis), gravity, diffusion, pressure and concentration gradients and also osmosis or centrifugal forces. The individual samples (29) are detected in selected regions (8) inside or outside the separation medium (6) during their migration (online detection). As an alternative to online detection, data may be received in a 3D manner, after the migration has finished. To this end, an appropriate 3D image-taking process may be used. In the simplest case, the separation medium (6) is cut into disks which are then evaluated by means of a 2D receiving process. In this case, the fractions of the samples are also preparatively accessible. In addition to or alternatively to image-taking, a fraction collector may be attached to an interface of the separation medium (6).

摘要翻译： 本发明涉及用于在三维分离介质（6）内并行分馏多个单个样品（29）的方法和装置，其中随后的工艺步骤被执行：第一空间（2），其基本上跨过 所有三个空间坐标都包含分离介质（6）。 多个单个样本（29）布置在第一空间（2）的界面附近，各个样本（29）基本上以平面方式布置，即它们的特定重心位置由两个 坐标 在一个或多个物理参数的影响下，个体样品（29）能够通过分离介质（6）基本上垂直于其应用的区域迁移，并且在该过程中根据它们的一种或多种性质 。 合适的物理参数是例如电力（电泳），重力，扩散，压力和浓度梯度以及渗透或离心力。 在分离介质（6）内部或外部的选定区域（8）中，在移动（在线检测）期间检测各个样品（29）。 作为在线检测的替代方案，迁移完成后，可以以3D方式接收数据。 为此，可以使用适当的3D图像拍摄过程。 在最简单的情况下，将分离介质（6）切割成盘，然后通过2D接收处理来评估。 在这种情况下，样品的分数也是可制备的。 除了摄影或替代摄影，分级收集器可以附着到分离介质（6）的界面。