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公开(公告)号：US20230383256A1
公开(公告)日：2023-11-30
申请号：US18246471
申请日：2021-09-23
Applicant: Erypharm
Inventor： Guillaume ROUSSEAU , Luc DOUAY , Marie-Catherine GIARRATANA , Florent MATHIEU
CPC classification number: C12N5/0641 , A61K35/18 , C12N2506/11 , C12N2511/00
Abstract: The invention relates to a process for producing cultured red blood cells from stem cells or cells of an immortalized cell line of the erythroid lineage.
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公开(公告)号：US20230212506A1
公开(公告)日：2023-07-06
申请号：US17607718
申请日：2020-06-30
Applicant: BASF SE
Inventor： Tobias Klein , Andreas Daub , Aydin Golabgir Anbarani
CPC classification number: C12N1/205 , C12N9/2417 , C12Y302/01001 , C12N2511/00
Abstract: The invention relates to a process for culturing microbial cells producing a product of interest comprising fed-batch pre-culture.
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公开(公告)号：US20230203101A1
公开(公告)日：2023-06-29
申请号：US17920526
申请日：2021-04-22
Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION , KOREA NATIONAL INSTITUTE OF HEALTH , BIOAPPLICATIONS INC
Inventor： In Hwan HWANG , Shijian SONG , Rezaul Islam Khan MD , Haiping DIAO , Eun Ju SOHN , Bo Hwa CHOI , Jang Hoon CHOI , Eun Young JANG , Young Jae LEE
IPC: C07K14/005 , C12N15/82
CPC classification number: C07K14/005 , C12N15/8258 , C12N2770/20022 , C12N2770/20071 , C12N2770/20034 , C12N2770/20043 , C12N2770/20051 , C12N2511/00
Abstract: The present invention relates to a recombinant spike protein of the COVID-19 virus forming a trimer and a method for mass-producing the recombinant spike protein, and more specifically to a method for designing a recombinant gene expressing a recombinant spike protein of the COVID-19 virus forming a trimer for the purposes of enhancing immunogenicity and effective antigen delivery, and a method for mass-producing the recombinant spike protein in plants.
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公开(公告)号：US20190194694A1
公开(公告)日：2019-06-27
申请号：US16065167
申请日：2016-12-23
Applicant: SELEXIS S.A.
Inventor： Nicolas Mermod , Pierre-Olivier Duroy , Sandra Bosshard , Philippe Le Mercier
CPC classification number: C12N15/907 , C12N5/0682 , C12N15/102 , C12N2310/20 , C12N2510/00 , C12N2511/00 , C12P21/00
Abstract: Disclosed are mammalian cells and mammalian cell lines that have a reduced load of remnants of past viral/retroviral infections and methods of producing and using the same.
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公开(公告)号：US20180320128A1
公开(公告)日：2018-11-08
申请号：US15773186
申请日：2016-11-09
Applicant: ARES TRADING S.A.
Inventor： DAVID BRUHLMANN , MARTIN JORDAN
CPC classification number: C12N1/38 , C07K19/00 , C12N5/0037 , C12N5/0682 , C12N15/85 , C12N2015/8518 , C12N2500/34 , C12N2501/90 , C12N2510/02 , C12N2511/00 , C12P21/005
Abstract: The present invention relates to methods and compositions for modulating glycosylation of recombinant proteins expressed by mammalian host cells during the cell culture process. Also disclosed are methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising a disaccharide or a trisaccharide, while keeping the osmolality constant.
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76.发明授权
公开(公告)号：US09944968B2
公开(公告)日：2018-04-17
申请号：US15000522
申请日：2016-01-19
Applicant: Biogen MA Inc. , Samsung Bioepis
Inventor： William Yang , Yao-Ming Huang , Kyle McElearney , Lia Tescione , James Lambropoulos , An Zhang , Valerie Tsang , Thomas Ryll , Sangil Lee , Dae Sung Lee
CPC classification number: C12P21/005 , C12N5/0018 , C12N2500/20 , C12N2500/30 , C12N2500/34 , C12N2500/40 , C12N2500/90 , C12N2501/04 , C12N2511/00
Abstract: The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
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公开(公告)号：US20180044405A1
公开(公告)日：2018-02-15
申请号：US15591022
申请日：2017-05-09
Applicant: Baxalta Incorporated , Baxalta GmbH
Inventor： Leopold Grillberger , Manfred Reiter , Wolfgang Mundt , Daniel Fleischanderl , Gregor Bramberger
CPC classification number: C07K14/755 , A61K9/0019 , A61K38/00 , A61K38/36 , A61K38/37 , C12N9/6489 , C12N2500/24 , C12N2511/00 , C12P21/00 , C12Y304/24087
Abstract: Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
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公开(公告)号：US20170369905A1
公开(公告)日：2017-12-28
申请号：US15534735
申请日：2015-12-11
Applicant: University of Copenhagen , Novo Nordisk A/S
Inventor： Henrik Clausen , Zhang Yang , Adnan Fevzi Halim , Eric Bennett , Carsten Behrens , Malene Bech Vester-Christensen , Shamim Herbert Rahman
IPC: C12N15/90 , C12P21/00 , C07K14/505 , C07K16/46
CPC classification number: C12N15/907 , C07K14/505 , C07K16/464 , C07K2317/24 , C07K2317/56 , C12N2501/24 , C12N2501/724 , C12N2511/00 , C12P21/005
Abstract: The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses.
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公开(公告)号：US20170349655A1
公开(公告)日：2017-12-07
申请号：US15626637
申请日：2017-06-19
Applicant: AbbVie Inc.
Inventor： Kartik Subramanian , Mayda Perez Thiele , Xiaobei Zeng , Chee Furng Wong , Christopher M. Chumsae
CPC classification number: C07K16/241 , C07K16/00 , C07K16/2803 , C07K2317/14 , C07K2317/21 , C07K2317/40 , C07K2317/526 , C12N5/0018 , C12N2500/22 , C12N2500/32 , C12N2511/00 , C12P21/00
Abstract: The instant invention relates to the field of protein production and purification, and in particular to compositions and processes for controlling the distribution or amount of lysine variants expressed by host cells, as well as to compositions and processes for controlling the amount of lysine variants present in purified preparations.
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80.发明申请
公开(公告)号：US20170327786A1
公开(公告)日：2017-11-16
申请号：US15523009
申请日：2015-10-30
Applicant: Biogen MA Inc.
Inventor： Yao-Ming HUANG , William C. YANG , Alan GILBERT , Kyle MCELEARNEY
IPC: C12N5/00 , C07K16/28 , C07K14/565 , C07K14/705 , C07K16/24 , C07K16/18 , C07K16/42 , C07K14/525 , C12P21/00 , C07K16/00
CPC classification number: C12N5/0018 , C07K14/525 , C07K14/565 , C07K14/70575 , C07K16/00 , C07K16/18 , C07K16/241 , C07K16/246 , C07K16/2839 , C07K16/2887 , C07K16/42 , C07K2317/14 , C12N2500/33 , C12N2500/38 , C12N2501/845 , C12N2501/999 , C12N2510/02 , C12N2511/00 , C12P21/00
Abstract: The present invention pertains to a cell culture medium comprising hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine as media supplements which is shown to control viability, growth, and waste byproduct accumulation. The present invention further pertains to a method of producing a polypeptide of interest in a large scale cell culture containing hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine.
