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公开(公告)号：US09557323B2
公开(公告)日：2017-01-31
申请号：US13581483
申请日：2011-09-26
Applicant: Sofia A. Casares , Teodor D. Brumeanu , Thomas L. Richie
Inventor： Sofia A. Casares , Teodor D. Brumeanu , Thomas L. Richie
IPC: A01K67/027 , G01N33/50 , A61D19/04
CPC classification number: G01N33/5088 , A01K67/0278 , A01K2217/052 , A01K2217/15 , A01K2227/105 , A01K2267/03 , A61D19/04
Abstract: Provided is a transgenic animal model for testing immunogenicity and protective efficacy of human vaccines and the method for generating such a multitransgenic animal. Also disclosed are methods for screening compositions for human vaccine development. More specifically, a mouse model capable of expressing human leukocyte antigen DR4, and human costimulatory molecules (CD80) upon infusion of human HLA-matched hematopoietic stem cells, which can develop into a functional human immune system is provided.
Abstract translation: 提供了用于测试人类疫苗的免疫原性和保护功效的转基因动物模型以及产生这种多转基因动物的方法。 还公开了筛选用于人类疫苗开发的组合物的方法。 更具体地，提供了能够发展成功能性人免疫系统的能够表达人白细胞抗原DR4的小鼠模型，以及输入人类HLA匹配造血干细胞后的人共刺激分子（CD80）。
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公开(公告)号：US20170016879A1
公开(公告)日：2017-01-19
申请号：US15276747
申请日：2016-09-26
Applicant: Premium Genetics (UK) LTD.
Inventor： Amy L. Morjal , Christopher R. Knutson , Daniel Mueth , Joseph Plewa , Evan Tanner
CPC classification number: C12N5/0612 , A61D19/04 , B82Y5/00 , B82Y10/00 , C12N5/061 , G01N1/30 , G01N15/14 , G01N15/1425 , G01N21/6428 , G01N33/5005 , G01N33/689 , G01N2015/0065 , G01N2015/1006 , G01N2015/1081 , G01N2015/149 , G01N2021/6439
Abstract: The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided.
Abstract translation: 本发明提供新的细胞染色方法，例如牛精子，在活力增强温度下使用电穿孔或渗透压浓度处理。 此外，还提供了使用新型细胞染色方法特别适用于分选牛精子的高效细胞分选方法。
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公开(公告)号：US20160374786A1
公开(公告)日：2016-12-29
申请号：US15133414
申请日：2016-04-20
Applicant: Quinn A. Gavaga
Inventor： Quinn A. Gavaga
IPC: A61D19/04 , A61B17/435
CPC classification number: A61D19/04 , A61B17/435
Abstract: An embryo from a female animal is transferred to another animal by determining presence of an embryo in the uterus of a donor animal by ultra-sonic imaging and inserting an endoscope vaginally into the uterus to a location adjacent the embryo. A tool of the endoscope projects to a position to extract the embryo washed into a container of the tool which is then closed by moving a closure part to enclose the embryo and extracting the endoscope to remove the embryo for transfer to a recipient animal. The fluid into the container can be controlled in pressure to maintain a required pressure generally matching that inside the uterus.
Abstract translation: 通过超声波成像确定供体动物的子宫中的胚胎的存在并将内窥镜阴道内插入到与胚胎相邻的位置，从而将来自雌性动物的胚胎转移到另一种动物。 内窥镜的工具突出到将被洗涤的胚胎提取到工具的容器中的位置，然后通过移动封闭部分来封闭胚胎并提取内窥镜以移除胚胎以转移到受体动物而将其封闭。 进入容器的流体可以被压力控制以保持通常与子宫内部匹配的所需压力。
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54.发明申请
公开(公告)号：US20160354068A1
公开(公告)日：2016-12-08
申请号：US15151151
申请日：2016-05-10
Applicant: Andrew I. Schneider
Inventor： ANDREW I. SCHNEIDER , Mark McGlothlin , Scott Herrick
CPC classification number: A61B42/10 , A61B17/32 , A61B18/14 , A61B42/00 , A61B90/30 , A61B2017/00438 , A61B2017/00876 , A61B2017/306 , A61B2017/320008 , A61B2018/00595 , A61B2217/005 , A61B2217/007 , A61D19/04
Abstract: A method of manufacturing a glove that includes discrete elements and component systems, such as, but not limited to, lights, electrical cautery, suction, and irrigation, attached to the surgical glove. The gloves produced by this method can be used as surgical gloves or for other industrial applications. In a particular application, the method includes providing a former comprising a hand-shaped portion and a first surgical system comprising a first surgical instrument, and a first switch for controlling the first surgical system. The former includes a first depression for receiving the first surgical system. The first depression is adapted to produce an interference fit with at least a portion of the first surgical system. The first surgical system can be loaded into the first depression and a polymer coating can be applied over the loaded former to form a surgical glove.
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公开(公告)号：US20160319242A1
公开(公告)日：2016-11-03
申请号：US15209516
申请日：2016-07-13
Inventor： Ralf KÜHN , Wolfgang WURST , Oskar ORTIZ SANCHEZ
CPC classification number: C12N5/0609 , A01K67/0275 , A01K2217/072 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A61D19/04 , C12N9/22 , C12N15/102 , C12N15/89 , C12N15/907 , C12N2800/80
Abstract: The present invention relates to a method of producing a non-human, mammalian oocyte carrying a modified target sequence in its genome, the method comprising the steps of introducing into a non-human, mammalian oocyte: (a) a clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated protein 9 (Cas9 protein) or a nucleic acid molecule encoding said Cas9 protein; and (b-i) a target sequence specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracr RNA) or a nucleic acid molecule encoding said RNAs; or (b-ii) a chimaeric RNA sequence comprising a target sequence specific crRNA and tracrRNA or a nucleic acid molecule encoding said RNA; wherein the Cas9 protein introduced in (a) and the RNA sequence(s) introduced in (b-i) or (b-ii) form a protein/RNA complex that specifically binds to the target sequence and introduces a single or double strand break within the target sequence. The present invention further relates to the method of the invention, wherein the target sequence is modified by homologous recombination with a donor nucleic acid sequence further comprising the step: (c) introducing a nucleic acid molecule into the cell, wherein the nucleic acid molecule comprises the donor nucleic acid sequence and regions homologous to the target sequence. The present invention also relates to a method of producing a non-human mammal carrying a modified target sequence in its genome.
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公开(公告)号：US20160150766A1
公开(公告)日：2016-06-02
申请号：US14906317
申请日：2014-07-23
Applicant: Jennifer BARFIELD , Brandon L. WEBB
Inventor： Jennifer BARFIELD , Brandon L. WEBB
CPC classification number: A01K67/02 , A01K67/027 , A01K2207/20 , A01K2227/101 , A61D19/02 , A61D19/04 , A61K31/57 , A61K38/24
Abstract: This invention relates to a method for reducing the generation interval and advancing genetic gain in bovines via pregnancies created by oocytes harvested from select prepubertal female calves less than six months of age and/or from smaller framed bovine breeds less than ten months of age, and alternatively less than nine months of age. Said animals are treated with a hormone regiment to advance the first pubertal estrus in order to retrieve oocytes for fertilization and implantation. Oocytes are collected from said animals via laparoscopic aspiration. In the case of oocytes that have not matured in vivo, the oocytes are transferred to and cultured to maturation. Once matured, oocytes are fertilized, and the resultant embryos are transferred to synchronized recipients.
Abstract translation: 本发明涉及通过从少于六个月龄的选择性青春期前雌性小牛收获的卵母细胞和/或少于十个月龄的较小框架的牛品种产生的怀孕产生的妊娠来减少牛的产生间隔和促进遗传增益的方法，以及 或者不到九个月。 所述动物用激素团治疗以促进第一次青春期发情，以便获取用于受精和植入的卵母细胞。 通过腹腔镜抽吸从所述动物收集卵母细胞。 在体内未成熟的卵母细胞的情况下，将卵母细胞转移并培养成熟。 一旦成熟，卵母细胞受精，并将所得的胚胎转移到同步的受体。
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57.发明授权Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow 有权在母猪输卵管中插入或获得配子，胚胎或任何其他类型溶液的流体的装置和方法
公开(公告)号：US09277981B2
公开(公告)日：2016-03-08
申请号：US13132518
申请日：2009-09-24
Applicant: Juan Maria Vazquez Rojas , Emilio Martinez Garcia , Jose Luis Vazquez Rojas , Jorge Roca Aleu
Inventor： Juan Maria Vazquez Rojas , Emilio Martinez Garcia , Jose Luis Vazquez Rojas , Jorge Roca Aleu
CPC classification number: A61D19/04 , A61B10/0045 , A61B10/0291 , A61B17/3478 , A61B46/13 , A61D19/027
Abstract: The device comprises an injection system, which is made up of a rigid external tubular body (1) that enables it to be inserted into the abdominal cavity of a sow. This body is, in turn, covered by a sterile sleeve (2) that enables it to be used in different animals. A flexible duct (3) runs coaxially and its distal end connects with a bevelled needle (4) that permits insertion in the oviduct (12) at an angle of 45°. The proximal end of the flexible tube connects with a device (6) that includes a sheath (7) closed at the other end by a piston (8) that slides along the inside of the sheath and that enables the precision insertion of low-volume liquids.
Abstract translation: 该装置包括注射系统，该注射系统由刚性的外部管状体（1）构成，使其能够插入到母猪的腹腔中。 这个身体又被一个无菌套管（2）所覆盖，使其能够用于不同的动物。 柔性管道（3）同轴运行，其远端与倾斜的针头（4）连接，该针头允许以45°的角度插入输卵管（12）中。 柔性管的近端与包括由另一端封闭的护套（7）的装置（6）连接，活塞（8）沿着护套的内部滑动，并且能够精确地插入低体积 液体。
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公开(公告)号：US20160030010A1
公开(公告)日：2016-02-04
申请号：US14448074
申请日：2014-07-31
Applicant: PALO ALTO RESEARCH CENTER INCORPORATED
Inventor： David Mathew Johnson , Scott A. Uhland , Ramkumar Abhishek , Robert Thomas Krivacic , Martin Sheridan
IPC: A61B10/00 , A61D19/04 , A61B5/1459 , A61B5/1455 , A61M31/00 , A61B5/11 , A61B5/03 , A61B5/07 , A61B5/00 , A61D19/02 , A61B5/0205
CPC classification number: A61B10/0012 , A61B5/02055 , A61B5/02416 , A61B5/035 , A61B5/076 , A61B5/1032 , A61B5/1118 , A61B5/14551 , A61B5/14552 , A61B5/1459 , A61B5/4839 , A61B2010/0016 , A61B2010/0019 , A61B2503/40 , A61D17/002 , A61D19/027 , A61D19/04
Abstract: Devices, systems, and methods for detecting estrus in subjects are provided. Devices include a housing configured for intravaginal/intrauterine deployment and retention and a sensor disposed in or on the housing, and are configured to use condition information sensed by the sensor to determine an estrus condition of the subject. Methods include deploying a device in the subject, sensing the condition information, and determining an estrus condition using the condition information. Systems include a device configured to communicate with a base station and/or with other implanted devices, which are located within a reception radius thereof, regarding the determined estrus condition.
Abstract translation: 提供了用于检测受试者发情的装置，系统和方法。 设备包括被配置用于阴道内/子宫内展开和保持的壳体和设置在壳体中或壳体上的传感器，并且被配置为使用由传感器感测的状态信息来确定受试者的发情状况。 方法包括在受检者中部署装置，感测状况信息，以及使用条件信息确定发情状况。 系统包括被配置为关于所确定的发情状况与位于其接收半径内的基站和/或与其他植入设备通信的设备。
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公开(公告)号：US20150307943A1
公开(公告)日：2015-10-29
申请号：US14754729
申请日：2015-06-30
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： Hasan Khatib
CPC classification number: C12Q1/6883 , A01K67/02 , A01K67/027 , A01K2227/101 , A01K2267/02 , A61D19/04 , A61K35/54 , C12Q1/68 , C12Q1/6888 , C12Q2600/124 , C12Q2600/156 , C12Q2600/16
Abstract: Single nucleotide polymorphic sites of the bovine MAP1B, PPP1R11, and DDX4 genes are associated with improved bull fertility as measured by e.g. sire conception rates. Nucleic acid molecules, arrays, kits, methods of genotyping and marker-assisted bovine breeding methods based on these SNPs are disclosed.
Abstract translation: 牛MAP1B，PPP1R11和DDX4基因的单核苷酸多态性位点与改良的公牛生育力相关联，如通过例如 父母受孕率。 公开了基于这些SNP的核酸分子，阵列，试剂盒，基因分型方法和标记辅助牛育种方法。
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公开(公告)号：US20150150667A1
公开(公告)日：2015-06-04
申请号：US14611040
申请日：2015-01-30
Applicant: The Jackson Laboratory
Inventor： Robert Taft , Michael V. Wiles
Abstract: The present invention provides methods and systems for efficient and cost-effective provision of mouse colony management services to customers. Described herein is a novel integrated approach to provide a plurality of mouse colony management services by utilizing a common core process shared by the plurality of services.
Abstract translation: 本发明提供了向客户有效和成本有效地提供小鼠集落管理服务的方法和系统。 这里描述了一种新颖的集成方法，通过利用由多个服务共享的共同核心过程来提供多个鼠标集群管理服务。
