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公开(公告)号：US20090280532A1
公开(公告)日：2009-11-12
申请号：US12463351
申请日：2009-05-08
Applicant: Stephen F. GORFIEN , Richard M. FIKE , Glenn P. GODWIN , Joyce L. DZIMIAN , David A. EPSTEIN , Dale GRUBER , Don McCLURE , Paul J. PRICE
Inventor： Stephen F. GORFIEN , Richard M. FIKE , Glenn P. GODWIN , Joyce L. DZIMIAN , David A. EPSTEIN , Dale GRUBER , Don McCLURE , Paul J. PRICE
IPC: C12P21/00
CPC classification number: C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
Abstract: The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
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公开(公告)号：US20090263371A1
公开(公告)日：2009-10-22
申请号：US12227618
申请日：2006-05-31
Applicant: Frank Riske , Michael Hayes , Gary Lazarus
Inventor： Frank Riske , Michael Hayes , Gary Lazarus
CPC classification number: A61K38/47 , A61K31/718 , A61K38/00 , C08B31/12 , C12N9/2402 , C12N9/2471 , C12N9/96 , C12Y302/01018 , C12Y302/0102 , C12Y302/01023 , C12Y302/01045 , C12Y302/01052
Abstract: This disclosure provides methods and compositions for the promotion of enzymatic activity of Target Enzymes, including but not limited to oligosaccharide/polysaccharide enzymes, protein enzymes, polynucleotide enzymes. The methods involve use of a non-naturally occurring polysaccharide (including but not limited HES) for promoting the enzymatic activity of an enzyme in liquid milieu, wherein the concentration of the polysaccharide in the composition comprising the Target Enzyme is from about 0.01% to about 55% w/v.
Abstract translation: 本公开提供了用于促进靶酶的酶活性的方法和组合物，包括但不限于寡糖/多糖酶，蛋白质酶，多核苷酸酶。 所述方法包括使用非天然存在的多糖（包括但不限于HES）来促进酶在液体环境中的酶活性，其中包含目标酶的组合物中多糖的浓度为约0.01％至约 55％w / v。
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公开(公告)号：US20090099079A1
公开(公告)日：2009-04-16
申请号：US12205694
申请日：2008-09-05
Applicant: Mark A. Emalfarb , Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
Inventor： Mark A. Emalfarb , Alexander Vasilievich Gusakov , Peter J. Punt , Jan Cornelis Verdoes , Arkady Panteleimonovich Sinitsyn , Elena Vlasenko , Sandra Wihelmina Agnes Hinz , Mark Gosink , Zhijie Jiang , Jacoba Van der Meij
IPC: A61K38/16 , C07H21/00 , C07K14/00 , C07K16/44 , C12N9/42 , C12N15/64 , C12N5/10 , C12N1/15 , C12N1/13 , C12N1/21 , C12P21/00 , C12N9/26 , C12N9/24 , C12N9/20 , C12N9/16 , C12P19/02 , C12P7/04 , C12P7/06 , D06L3/11 , C09B67/42 , C12S11/00 , C11D7/42 , D06Q1/02 , A23K1/165 , A23L1/48 , A61P29/00 , C12N1/06 , C12P13/04 , C12P7/26
CPC classification number: C12N9/248 , C07H21/00 , C12N9/2402 , C12N9/2408 , C12N9/2434 , C12N9/2471 , C12Y301/01072 , C12Y302/01023 , C12Y302/01037 , C12Y302/01055 , C12Y302/01139 , C12Y302/01151 , C12Y302/01155 , Y02E50/16 , Y02E50/17
Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
Abstract translation: 本发明涉及新型酶及其生产方法。 更具体地，本发明涉及各种真菌酶。 描述了编码这种酶，组合物，重组和遗传修饰的宿主细胞的核酸分子和使用方法。 本发明还涉及将木质纤维素生物质转化成可降解木质纤维素材料的酶和酶的新组合（包括提供来自植物生物质的糖的协同释放的酶）的方法。 本发明还涉及通过细胞壁降解释放细胞含量的方法。 本发明还涉及在各种其它方法中使用新型酶和这些酶的组合物的方法，包括洗涤衣物，洗涤剂方法，生物精制，纸和纸浆的脱墨和生物漂白以及废物流的处理。
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44.发明申请METHODS AND COMPOSITIONS FOR ENHANCING DELIVERY OF DOUBLE-STRANDED RNA OR A DOUBLE-STRANDED HYBRID NUCLEIC ACID TO REGULATE GENE EXPRESSION IN MAMMALIAN CELLS 审中-公开用于增强递送双链RNA或双重混合核酸以调节哺乳动物细胞中基因表达的方法和组合物
公开(公告)号：US20080261304A1
公开(公告)日：2008-10-23
申请号：US12013274
申请日：2008-01-11
Applicant: Mohammad Ahmadian , Kunyuan Cui , Lishan Chen , Shu-Chih Chen , Michael E. Houston
Inventor： Mohammad Ahmadian , Kunyuan Cui , Lishan Chen , Shu-Chih Chen , Michael E. Houston
CPC classification number: A61K45/06 , A61K31/713 , A61K38/00 , A61K47/554 , A61K48/005 , C07K14/00 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N15/87 , C12N2310/14 , C12N2310/3513 , C12N2310/3515 , C12N2320/30 , C12N2320/32 , C12Y302/01023
Abstract: A double-stranded RNA, preferably a small, interfering (si)RNA or a siHybrid, to which cholesterol moieties are linked.
Abstract translation: 胆固醇部分连接的双链RNA，优选小的干扰（si）RNA或siHybrid。
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45.发明申请
公开(公告)号：US20080248019A1
公开(公告)日：2008-10-09
申请号：US11823333
申请日：2007-06-25
Applicant: Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
Inventor： Hyun Ah Kang , Jae Kap Jeong , Ohsuk Kwon , Doo-Byoung Oh , Seonghun Kim
CPC classification number: C12Y302/01023 , A61K38/00 , C12N9/2471
Abstract: The present invention relates to a novel beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.
Abstract translation: 本发明涉及来自肺炎链球菌的新型β-半乳糖苷酶，表现出酶活性的BgaC蛋白质及其使用方法。 该蛋白质可用于糖链的修饰和分析，并用作抗癌剂。
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公开(公告)号：US20080070231A1
公开(公告)日：2008-03-20
申请号：US11533522
申请日：2006-09-20
Applicant: Phillip P. Franciskovich , William A. Yirava , Tricia A. Cregger
Inventor： Phillip P. Franciskovich , William A. Yirava , Tricia A. Cregger
CPC classification number: C12N15/75 , C12N9/2471 , C12Y302/01023
Abstract: The disclosed technology relates to a genetically engineered biological indicator, comprising: at least one test organism and at least one reporter gene suitable for producing an indicator enzyme, the reporter gene being taken up by the test organism; and at least one repressor gene that inhibits expression of the reporter gene until the reporter gene is exposed to at least one inducer. A process and an apparatus for using the biological indicator are disclosed.
Abstract translation: 所公开的技术涉及基因工程生物指示剂，其包含：至少一种测试生物体和至少一种适用于产生指示酶的报告基因，所述报道基因被测试生物体吸收; 以及抑制报告基因表达的至少一个抑制基因，直到报告基因暴露于至少一种诱导物。 公开了一种使用生物指示剂的方法和装置。
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公开(公告)号：US20080050356A1
公开(公告)日：2008-02-28
申请号：US11786250
申请日：2007-04-11
Applicant: Steve Pakola , Marc De Smet
Inventor： Steve Pakola , Marc De Smet
CPC classification number: A61K38/484 , A61K45/06 , C12Y302/01023 , C12Y302/01035 , C12Y304/21007 , C12Y304/24007 , C12Y402/02004 , C12Y402/02005 , C12Y402/02019 , A61K2300/00
Abstract: Methods of treating or preventing a disorder, or a complication of a disorder, of an eye of a subject, comprising contacting a vitreous and/or aqueous humor with a composition comprising a truncated form of plasmin comprising a catalytic domain of plasmin (TPCD) are disclosed. TPCDs include, but are not limited to, miniplasmin, microplasmin and derivatives and variants thereof. The methods of the invention can be used to reduce the viscosity of the vitreous, liquefy the vitreous, induce posterior vitreous detachment, reduce hemorrhagic blood from the eye, clear or reduce materials toxic to the eye, clear or reduce intraocular foreign substances from the eye, increase diffusion of a composition administered to an eye, reduce extraretinal neovascularization and any combinations thereof. The method can be used in the absence of, or as an adjunct to, vitrectomy.
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公开(公告)号：US07294498B2
公开(公告)日：2007-11-13
申请号：US10121032
申请日：2002-04-09
Applicant: Edward J. Bylina , Ronald V. Swanson , Eric J. Mathur , David E. Lam
Inventor： Edward J. Bylina , Ronald V. Swanson , Eric J. Mathur , David E. Lam
CPC classification number: C12N9/2491 , C12N9/2402 , C12N9/2445 , C12N9/2454 , C12N9/2457 , C12N9/2465 , C12N9/2468 , C12N9/2494 , C12P19/02 , C12Y302/01021 , C12Y302/01022 , C12Y302/01023 , C12Y302/01025 , C12Y302/01041
Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.
Abstract translation: 公开了衍生自各种热球菌属，葡萄球菌属和火球菌属的热稳定性糖苷酶。 酶由天然或重组宿主细胞产生，可用于食品加工业，制药工业，纺织工业，洗涤剂行业和烘焙行业。
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公开(公告)号：US07101709B2
公开(公告)日：2006-09-05
申请号：US10199189
申请日：2002-07-19
Applicant: Samuel Weiss , Brent Reynolds , Joseph P. Hammang , E. Edward Baetge
Inventor： Samuel Weiss , Brent Reynolds , Joseph P. Hammang , E. Edward Baetge
CPC classification number: G01N33/5026 , A01K2217/05 , A61K35/30 , A61K48/00 , C07K14/47 , C07K14/475 , C07K14/82 , C12N5/0623 , C12N9/0065 , C12N9/2471 , C12N9/88 , C12N2500/25 , C12N2500/46 , C12N2500/90 , C12N2501/11 , C12N2501/148 , C12N2501/15 , C12N2501/392 , C12N2501/91 , C12N2503/02 , C12N2510/00 , C12Y302/01023 , C12Y402/01011 , G01N33/5008 , G01N33/5017 , G01N33/5023 , G01N33/5058 , G01N33/5073 , G01N2500/00 , G01N2510/00
Abstract: The invention discloses methods of proliferation and differentiation of multipotent neural stem cells. Also provided are methods of making cDNA libraries and methods of screening biological agents which affect proliferation differentiation survival phenotype or function of CNS cells.
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公开(公告)号：US07081355B2
公开(公告)日：2006-07-25
申请号：US10387388
申请日：2003-03-14
Applicant: Flemming Jørgensen , Ole Cai Hansen , Peter Stougaard
Inventor： Flemming Jørgensen , Ole Cai Hansen , Peter Stougaard
IPC: C12P19/44 , C12N9/10 , C12N9/26 , A23C17/02 , A23C19/032
CPC classification number: C12Y302/01023 , A01K2217/05 , C12N9/2471
Abstract: The present invention concerns a new β-galactosidase with transgalactosylating activity isolated from Bifidobacterium bifidum and a truncated enzyme where the C-terminal end of the β-galactosidase protein has been deleted, resulting in an enzyme with a higher transgalactosylating activity than hydrolase activity. When lactose is used as a substrate, galacto-oligosaccharides are products of the transgalactosylase activity. Galacto-oligosaccharides enhance growth of health-promoting Bifidobacterium that may be used in a number of applications in the dairy industry.
