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公开(公告)号：US20170135337A1
公开(公告)日：2017-05-18
申请号：US15419678
申请日：2017-01-30
Applicant: CORNING INCORPORATED
Inventor： Jessica Monique Brown , Nicholas Michael Illsley , Gregory Roger Martin , Ana Marie del Pilar Pardo , Allison Jean Tanner
IPC: A01N1/02
CPC classification number: A01N1/0284 , A01N1/0242 , A01N1/0263 , A01N1/0268 , A01N1/0273 , A61J1/05 , A61J1/1412 , A61J1/16 , A61M5/145 , A61M5/2425 , C12M23/48 , C12M33/04 , C12M45/22
Abstract: An article including, either: a frame having an interior cavity, and a flexible liner situated within the interior cavity; or a deformable frame having an interior cavity, as defined herein. Either frame can receive a sample, such as liquid having suspended live cells, and cooling freezes the sample to a solid sample. The solid sample can be readily ejected from the article having either a frame with the flexible liner, or the deformable frame, without extensive thawing. The solid sample can be rapidly displaced from the article by the methods and apparatus disclosed herein.
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公开(公告)号：US20140106444A1
公开(公告)日：2014-04-17
申请号：US14132749
申请日：2013-12-18
Applicant: CORNING INCORPORATED
Inventor： Gregory Roger Martin , Allison Jean Tanner
IPC: C12N5/071
CPC classification number: C12M23/08 , C12M23/04 , C12M23/24 , C12M23/34 , C12M23/38 , C12M23/58 , C12M25/02 , C12M25/04 , C12M25/06 , C12M29/04 , C12N5/0602
Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.
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公开(公告)号：US20130260454A1
公开(公告)日：2013-10-03
申请号：US13904171
申请日：2013-05-29
Applicant: Corning Incorporated
Inventor： Gregory Roger Martin , Allison Jean Tanner
IPC: C12M1/12
CPC classification number: C12M23/08 , C12M23/04 , C12M23/24 , C12M23/34 , C12M23/38 , C12M23/58 , C12M25/02 , C12M25/04 , C12M25/06 , C12M29/04 , C12N5/0602
Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.
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公开(公告)号：US20240002778A1
公开(公告)日：2024-01-04
申请号：US18368281
申请日：2023-09-14
Applicant: CORNING INCORPORATED
Inventor： Gregory Roger Martin , Allison Jean Tanner
CPC classification number: C12M47/02 , C12M33/14 , C12N5/0075
Abstract: A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.
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公开(公告)号：US20230383226A1
公开(公告)日：2023-11-30
申请号：US18031774
申请日：2021-10-27
Applicant: CORNING INCORPORATED
Inventor： Gregory Roger Martin , Allison Jean Tanner
CPC classification number: C12M23/16 , C12M23/12 , C12M23/20 , C12M41/46 , C12N5/0062 , C12M23/38 , C12N2513/00
Abstract: Microcavity plates (10) comprise a microcavity substrate and a base (303) comprising a bottom grid comprising a plurality of grid segments arranged in ordered rows and columns to form a plurality of openings, and an open well (307) comprising a plurality of sidewalls (309) extending vertically from a perimeter of the bottom grid. The microcavity substrate comprises a plurality of microcavities (315) arranged in ordered rows and columns that align with the plurality of openings in the bottom grid, each microcavity comprising a cavity disposed within an opening of the bottom grid. The microcavity plate may further comprise an upper grid (405) for placement on top of the bottom grid delineating the ordered rows and columns of the base.
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公开(公告)号：US11788051B2
公开(公告)日：2023-10-17
申请号：US16061964
申请日：2016-12-13
Applicant: Corning Incorporated
Inventor： Gregory Roger Martin , Allison Jean Tanner
CPC classification number: C12M47/02 , C12M33/14 , C12N5/0075
Abstract: A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.
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公开(公告)号：US11767499B2
公开(公告)日：2023-09-26
申请号：US16628810
申请日：2018-07-13
Applicant: CORNING INCORPORATED
Inventor： Gregory Roger Martin , Ana Maria Del Pilar Pardo , Michael Kurt Schaefer , Allison Jean Tanner
CPC classification number: C12M23/16 , B01L3/502715 , C12M23/08 , C12M23/24 , C12M29/04 , B01L2300/0681 , B01L2300/12
Abstract: A cell culture vessel (100) includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate (215) of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (220), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.
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公开(公告)号：US20230272325A1
公开(公告)日：2023-08-31
申请号：US18018202
申请日：2021-07-14
Applicant: CORNING INCORPORATED
Inventor： Thomas Albert Cloutier , Gregory Roger Martin
Abstract: A multi-position support for a multi-layer cell culture apparatus includes a primary base that rests against a support member in an upright configuration. A support surface is offset vertically from the primary base in the upright configuration. The support surface supports the multi-layer cell culture apparatus with the multi-layer cell culture apparatus located thereon. An intermediate surface extends between the primary base and the support surface. The intermediate surface meets the primary base at an interface that extends at an oblique angle to sides of the primary base. The multi-position support has a tilted configuration where the multi-position support is rotated about the interface such that the support surface is closer to the support member than in the upright configuration with the support surface supporting the multi-layer cell culture apparatus thereon.
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公开(公告)号：US20230257690A1
公开(公告)日：2023-08-17
申请号：US18018418
申请日：2021-07-28
Applicant: CORNING INCORPORATED
Inventor： Scott Matthew Bennett , Thomas Albert Cloutier , William Joseph Lacey , Gregory Roger Martin , Allison Jean Tanner
Abstract: A cell culture system for culturing cells is provided. The cell culture system including a cell culture vessel with a cell culture chamber for culturing cells, an inlet through which liquid can flow for filling the cell culture vessel, and an outlet through which fluid can exit the cell culture vessel. The system also includes at least one fill sensor arranged to detect liquid within the cell culture vessel reaching a fill level at a predetermined position of the cell culture vessel during filling of the cell culture vessel, where the at least one fill sensor can generate a detection signal when liquid within the cell culture vessel reaches the fill level. Also included is an actuator to change an orientation of the cell culture vessel in response to the detection signal.
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公开(公告)号：US20230012125A1
公开(公告)日：2023-01-12
申请号：US17780448
申请日：2020-11-09
Applicant: CORNING INCORPORATED
Inventor： John Wilfred Coddaire , Maryanne De Chambeau , James Thomas Eickmann , Paula Mary Flaherty , Anthony Glenn Frutos , Vasiliy Nikolaevich Goral , Angela Langer Julien , Marshall Jay Kosovsky , Brent Ravaughn Lanterman , Gregory Roger Martin , Christie Leigh McCarthy , John Shyu , Tora Ann-Beatrice Eline Sirkka , Allison Jean Tanner , Kimberly Ann Titus , Timothy James Wood
Abstract: A sensing cell culture vessel having one or more sensors on or in the vessel is configured to collect readings of various parameters or characteristics of a cell culture located within the sensing cell culture vessel and transmit the readings. The sensing cell culture vessels may be accompanied by a sensing plate having means for reading the one or more sensors and transmitting the one or more sensors to a server hosting electronic lab notebook for analyzing and storage of the readings. Sensing plates may further be equipped with cameras for imaging cell cultures located in the sensing cell culture vessels and transmitting the images to the server hosting the electronic lab notebook for analyzing and storage of the images. Embodiments of the invention allow for the continuous and automatic monitoring of cell cultures.
