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公开(公告)号：US20230032322A1
公开(公告)日：2023-02-02
申请号：US17885805
申请日：2022-08-11
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Zhengqi Zhang , Yuetian Yan , Shunhai Wang
Abstract: The present invention relates to the field of protein characterization, and in particular to methods for identifying critical quality attributes of therapeutic proteins by implementing a workflow including using a competitive binding assay with insufficient capture molecule followed by LC-MS.
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公开(公告)号：US11548932B2
公开(公告)日：2023-01-10
申请号：US17841349
申请日：2022-06-15
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Shunhai Wang , Ning Li , Shawn Lawrence , Amy Johnson , Meghan Casey , Jaimie Grapel , Hunter Chen , Amardeep Singh Bhupender Bhalla
IPC: C07K1/36 , C07K1/16 , C07K1/18 , C07K14/71 , A61K38/18 , G01N30/80 , C12P21/06 , C12N5/00 , C07K1/113 , C07K16/22 , C12N15/66 , C12P21/02 , C07K16/28 , C07K1/22 , G01N30/02
Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
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公开(公告)号：US11542317B1
公开(公告)日：2023-01-03
申请号：US17842286
申请日：2022-06-16
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Shunhai Wang , Ning Li , Hunter Chen , Amardeep Singh Bhupender Bhalla
IPC: C07K14/71 , C12P21/06 , C07K1/113 , C07K1/16 , C07K1/36 , C12N5/00 , C07K1/18 , C07K1/22 , C12P21/02 , A61K38/18 , C07K16/28 , C12N15/66 , C07K16/22 , G01N30/80 , G01N30/02
Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for administering such compositions.
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公开(公告)号：US20220389081A1
公开(公告)日：2022-12-08
申请号：US17841349
申请日：2022-06-15
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Shunhai Wang , Ning Li , Shawn Lawrence , Amy Johnson , Meghan Casey , Jamie Mastrogiacomo , Hunter Chen , Amardeep Singh Bhupender Bhalla
IPC: C07K14/71 , A61K38/18 , C07K16/22 , C07K1/18 , C12N15/66 , C07K16/28 , C12P21/02 , G01N30/80 , C07K1/22 , C07K1/36 , C12P21/06 , C07K1/113 , C07K1/16 , C12N5/00
Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
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公开(公告)号：US11459374B2
公开(公告)日：2022-10-04
申请号：US17667330
申请日：2022-02-08
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Andrew Tustian , Ankit Vartak , Thomas Daly , Erica Pyles , Nisha Palackal , Shunhai Wang , Ning Li
IPC: C07K1/36 , C07K14/71 , C12P21/06 , C07K1/113 , C07K1/16 , C12N5/00 , C07K1/18 , C07K1/22 , C12P21/02 , A61K38/18 , C07K16/28 , C12N15/66 , C07K16/22 , G01N30/80 , G01N30/02
Abstract: The present disclosure pertains to methods for preparing a prefilled syringe and methods for preparing an auto injector prefilled syringe comprising aflibercept and methods for producing such aflibercept in chemically defined media.
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36.发明授权
公开(公告)号：US11366123B2
公开(公告)日：2022-06-21
申请号：US16260482
申请日：2019-01-29
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Shunhai Wang
Abstract: Compositions and methods for identifying glucuronylated protein drug products are provided.
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公开(公告)号：US11359213B2
公开(公告)日：2022-06-14
申请号：US16897617
申请日：2020-06-10
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Shunhai Wang
Abstract: Methods of determining the stoichiometry of a viral capsid and/or determining the heterogeneity of protein components in a viral capsid are disclosed.
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公开(公告)号：US20220169704A1
公开(公告)日：2022-06-02
申请号：US17677499
申请日：2022-02-22
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Shunhai Wang
Abstract: Systems and methods for characterizing low molecular weight (LMW) protein drug product impurities are provided. One embodiment uses hydrophilic interaction chromatography (HILIC) coupled to mass spectrometry analysis. After removal of the N-linked glycans from the protein drug product, for example an antibody drug product, the elution of LMW impurities from the HILIC column was determined by the size of the molecular weight species. In some embodiments, the HILIC separation is performed under denaturing conditions, making the detection of LMW forms using this method highly comparable to both SDS-PAGE and CE-SDS methods. LMW drug product impurities include, but are not limited to light chain, half antibody, H2L, H2, HL, HC, peptide backbone-truncated species, and combinations thereof.
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公开(公告)号：US20220098280A1
公开(公告)日：2022-03-31
申请号：US17458519
申请日：2021-08-26
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Shunhai Wang , Ning Li , Hunter Chen , Amardeep Singh Bhupender Bhalla
IPC: C07K14/71 , C12P21/06 , C07K1/113 , C07K1/16 , C07K1/36 , C12N5/00 , C07K1/18 , C07K1/22 , C12P21/02 , A61K38/18 , C07K16/28 , C12N15/66 , C07K16/22 , G01N30/80
Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
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公开(公告)号：US11180540B2
公开(公告)日：2021-11-23
申请号：US16996127
申请日：2020-08-18
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Andrew Tustian , Ankit Vartak , Thomas Daly , Erica Pyles , Nisha Palackal , Shunhai Wang , Ning Li , Matthew Franklin
IPC: C07K1/16 , C07K1/36 , C07K14/71 , C12P21/06 , C07K1/113 , C12N5/00 , C07K1/18 , C07K1/22 , C12P21/02 , A61K38/18 , C07K16/28 , C12N15/66 , C07K16/22 , G01N30/80 , G01N30/02
Abstract: The present disclosure pertains to compositions comprising aflibercept and methods for producing such compositions in chemically defined media and using chromatography to reduce amounts of certain aflibercept variants.
