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公开(公告)号：US11192894B2
公开(公告)日：2021-12-07
申请号：US16725602
申请日：2019-12-23
Applicant: PROMEGA CORPORATION
Inventor： Mary Hall , Thomas Kirkland , Poncho Meisenheimer , Rachel Friedman Ohana , Anton Shakhmin , Joel R. Walker , Wenhui Zhou
IPC: C07D487/04 , G01N33/58 , C12Q1/66
Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
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公开(公告)号：US20200239475A1
公开(公告)日：2020-07-30
申请号：US16725602
申请日：2019-12-23
Applicant: PROMEGA CORPORATION
Inventor： Mary Hall , Thomas Kirkland , Poncho Meisenheimer , Rachel Friedman Ohana , Anton Shakhmin , Joel R. Walker , Wenhui Zhou
IPC: C07D487/04 , C12Q1/66 , G01N33/58
Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
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公开(公告)号：US10577370B2
公开(公告)日：2020-03-03
申请号：US15829262
申请日：2017-12-01
Applicant: PROMEGA CORPORATION
Inventor： Mary Hall , Thomas Kirkland , Poncho Meisenheimer , Rachel Friedman Ohana , Anton Shakhmin , Joel R. Walker , Wenhui Zhou
IPC: C07D487/04 , G01N33/58 , C12Q1/66
Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
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公开(公告)号：US10246690B2
公开(公告)日：2019-04-02
申请号：US15877980
申请日：2018-01-23
Applicant: Promega Corporation
Inventor： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Michael R. Slater , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kate Qin Zhao , Kris Zimmerman
Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
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35.发明申请
公开(公告)号：US20190064192A1
公开(公告)日：2019-02-28
申请号：US16121248
申请日：2018-09-04
Applicant: Promega Corporation
Inventor： Carolyn W. Hitko , Thomas Kirkland , Thomas Machleidt , Rachel Friedman Ohana , Matt Robers , Keith Wood
IPC: G01N33/94 , G01N33/58 , G01N33/542
Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.
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公开(公告)号：US10024862B2
公开(公告)日：2018-07-17
申请号：US14104860
申请日：2013-12-12
Applicant: Promega Corporation
Inventor： Carolyn W. Hitko , Thomas Kirkland , Thomas Machleidt , Rachel Friedman Ohana , Matt Robers , Keith Wood
IPC: G01N33/58 , G01N33/50 , G01N33/542
Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, or portions, components, or subunits of bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.
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公开(公告)号：US20180029028A1
公开(公告)日：2018-02-01
申请号：US15591842
申请日：2017-05-10
Applicant: Promega Corporation
Inventor： Sergiy Levin , Rachel Friedman Ohana , Thomas Kirkland , Keith Wood
CPC classification number: B01J31/2404 , B01J31/24 , B01J31/28 , B01J2231/005 , B01J2531/824 , B01J2540/225 , B01J2540/32 , C07B63/04 , C07F15/006 , C07F15/0066
Abstract: Provided herein are palladium (Pd) catalysts with improved performance in biological environments. In particular, formulations, methods of preparations, and storage conditions are provided that provide improved performance of Pd catalysts under protein-rich conditions.
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公开(公告)号：US09873866B2
公开(公告)日：2018-01-23
申请号：US15414291
申请日：2017-01-24
Applicant: Promega Corporation
Inventor： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Michael R. Slater , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kate Qin Zhao , Kris Zimmerman
CPC classification number: C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
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39.发明申请POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION 有权编码具有增强动力学和功能表达的突变型水解蛋白的多核苷酸
公开(公告)号：US20150140636A1
公开(公告)日：2015-05-21
申请号：US14285327
申请日：2014-05-22
Applicant: Promega Corporation
Inventor： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Michael R. Slater , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kate Qin Zhao , Kris Zimmerman
IPC: C12N9/14
CPC classification number: C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
Abstract translation: 本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。
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40.发明申请COMPOSITIONS AND METHODS FOR CAPTURE OF CELLULAR TARGETS OF BIOACTIVE AGENTS 审中-公开用于捕获生物活性剂细胞目标的组合物和方法
公开(公告)号：US20140287427A1
公开(公告)日：2014-09-25
申请号：US14204868
申请日：2014-03-11
Applicant: Promega Corporation
Inventor： Carolyn W. Hitko , Robin Hurst , Thomas Kirkland , Dieter Klaubert , Poncho Meisenheimer , Nidhi Nath , Rachel Friedman Ohana , Paul Otto , Marjeta Urh , Tetsuo Uyeda , Keith Wood
IPC: G01N33/569
CPC classification number: G01N33/543 , G01N33/5005
Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
Abstract translation: 本发明提供用于捕获和鉴定生物活性剂的细胞靶标的组合物和方法。 特别地，本文提供的是用于捕获配体，细胞靶标（任选地用报告物标记），捕获蛋白质（任选地作为捕获二聚体存在），表面（例如显示，捕获配体，捕获蛋白质或捕获二聚体）的生物活性剂， 以及捕获和鉴定生物活性剂的细胞靶标的方法。
