公开(公告)号：US6063593A

公开(公告)日：2000-05-16

申请号：US747514

申请日：1996-11-12

Applicant: Erlinda Maria Gordon ,  Frederick L. Hall ,  W. French Anderson

Inventor： Erlinda Maria Gordon ,  Frederick L. Hall ,  W. French Anderson

IPC: C12N15/09 ,  A61K31/711 ,  A61K35/12 ,  A61K35/28 ,  A61K38/43 ,  A61K48/00 ,  C12N5/02 ,  C12N5/0775 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/02 ,  C12P21/06 ,  C12P21/04

CPC classification number: C12N5/0663 ,  A61K2035/124 ,  A61K48/00 ,  C12N2501/15 ,  G01N2333/495

Abstract: A method for expressing a recombinant protein from bone marrow-derived cells comprises the steps of treating the bone marrow-derived cells in vitro with a TGF.beta.1 protein, which selects a population of the cells for further treatment. The selected cells can then be expanded, after which a gene encoding a therapeutic protein can be inserted into the expanded cells and thereafter express the therapeutic protein. The transduced cells can then be introduced into a mammal to produce a therapeutic result.

Abstract translation: 用于从骨髓来源的细胞表达重组蛋白的方法包括以TGFβ1蛋白体外处理骨髓来源的细胞的步骤，所述TGFβ1蛋白选择细胞群进一步处理。 然后可以扩增选定的细胞，然后将编码治疗性蛋白质的基因插入扩增的细胞中，然后表达治疗性蛋白质。 然后将转导的细胞引入哺乳动物以产生治疗结果。

公开(公告)号：US07347998B2

公开(公告)日：2008-03-25

申请号：US11014629

申请日：2004-12-15

Applicant: Frederick L. Hall ,  Erlinda Maria Gordon

Inventor： Frederick L. Hall ,  Erlinda Maria Gordon

IPC: A01N63/00

CPC classification number: C07K14/005 ,  A61K48/00 ,  C07K14/755 ,  C07K2319/00 ,  C07K2319/01 ,  C12N15/86 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/857

Abstract: A viral or non-viral vector particle having a modified viral surface protein wherein the viral surface protein is modified to include a targeting polypeptide including a binding region which binds to an extracellular matrix component. Such vector particles are useful in delivering genes encoding therapeutic agents to cells located at the site of an exposed extracellular matrix component.

Abstract translation: 具有修饰的病毒表面蛋白质的病毒或非病毒载体颗粒，其中所述病毒表面蛋白被修饰为包括靶向多肽，所述靶向多肽包括结合细胞外基质组分的结合区域。 这样的载体颗粒可用于将编码治疗剂的基因递送至位于暴露的细胞外基质组分位点的细胞。

公开(公告)号：US06844191B2

公开(公告)日：2005-01-18

申请号：US10076981

申请日：2002-02-15

Applicant: Erlinda Maria Gordon ,  Frederick L. Hall ,  W. French Anderson

Inventor： Erlinda Maria Gordon ,  Frederick L. Hall ,  W. French Anderson

IPC: C12N15/09 ,  A61K31/711 ,  A61K35/12 ,  A61K35/28 ,  A61K38/43 ,  A61K48/00 ,  C12N5/02 ,  C12N5/0775 ,  C12N5/10 ,  C12P21/02 ,  C12Q1/02 ,  C12N5/00

CPC classification number: C12N5/0663 ,  A61K48/00 ,  A61K2035/124 ,  C12N2501/15 ,  G01N2333/495

Abstract: A method for expressing a recombinant protein from bone marrow-derived cells comprises the steps of treating the bone marrow-derived cells in vitro with a TGFβ1 protein, which selects a population of the cells for further treatment. The selected cells can then be expanded, after which a gene encoding a therapeutic protein can be inserted into the expanded cells and thereafter express the therapeutic protein. The transduced cells can then be introduced into a mammal to produce a therapeutic result.

Abstract translation: 用于从骨髓来源的细胞表达重组蛋白的方法包括以TGFbeta1蛋白体外处理骨髓来源的细胞的步骤，其选择细胞群进行进一步处理。 然后可以扩增选定的细胞，然后将编码治疗性蛋白质的基因插入扩增的细胞中，然后表达治疗性蛋白质。 然后将转导的细胞引入哺乳动物以产生治疗结果。