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21.
公开(公告)号:US20240254465A1
公开(公告)日:2024-08-01
申请号:US18605895
申请日:2024-03-15
Applicant: HUAZHONG AGRICULTURAL UNIVERSITY
Inventor: Shengsong XIE , Shuhong ZHAO , Xinyun LI , Sheng LI , Bingrong XU , Dagang TAO , Liangliang FU , Jinxue RUAN , Heng WANG , Yunlong MA
CPC classification number: C12N9/22 , C12N15/11 , C12N15/907 , C12N2310/20 , C12N2800/80
Abstract: A nucleic acid endonuclease with high activity and high heat resistance and a gene editing system mediated by the nucleic acid endonuclease are provided. Specifically, the present invention provides a nucleic acid endonuclease Gs12-7 with a wide temperature range identified by metagenomics combined with experiments, which has the advantages of high protein temperature tolerance, recognition of PAM sequences containing BTYV, and thus has a larger gene editing space and high activity and specificity in cleaving target DNA in the genome. The present invention establishes a nucleic acid visualization detection and genome targeted editing technology mediated by the CRISPR/Gs12-7 system, which has broad application prospects in the field of genome targeted modification and nucleic acid detection.
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公开(公告)号:US20240240207A1
公开(公告)日:2024-07-18
申请号:US18415999
申请日:2024-01-18
Applicant: The General Hospital Corporation
Inventor: J. Keith Joung , James Angstman , Shengdar Tsai
IPC: C12N15/90 , C07K14/005 , C07K14/195 , C12N9/02 , C12N9/10 , C12N9/16 , C12N9/22 , C12N9/96 , C12N15/01 , C12N15/10 , C12N15/11 , C12N15/63 , C12N15/85
CPC classification number: C12N15/907 , C07K14/005 , C07K14/195 , C12N9/0071 , C12N9/1007 , C12N9/16 , C12N9/22 , C12N9/96 , C12N15/01 , C12N15/102 , C12N15/1031 , C12N15/11 , C12N15/63 , C12N15/85 , C12Y301/21004 , C07K2319/00 , C07K2319/01 , C07K2319/80 , C12N2310/20 , C12N2710/00033 , C12N2770/00033 , C12N2800/80 , C12Y114/11 , C12Y201/01 , C12Y301/00
Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
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公开(公告)号:US20240240165A1
公开(公告)日:2024-07-18
申请号:US18563699
申请日:2022-05-25
Inventor: Raúl PEREZ-JIMENEZ , Borja ALONSO-LERMA
CPC classification number: C12N9/22 , C12N15/11 , C12N15/907 , G16B10/00 , C12N2310/20 , C12N2800/80
Abstract: The use of phylogenetic ancestral sequence reconstruction to generate new Cas enzymes with improved capabilities. By this strategy, ancestral variants of Cas9 proteins of currently existing species have been obtained, which can exhibit nickase activity separate from endonuclease activity, and relaxed, if not abolished, PAM requirement. Ability to use tracrRNA components of Cas9 gRNAs from a wide variety of existing bacterial species has also been observed.
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公开(公告)号:US20240229081A1
公开(公告)日:2024-07-11
申请号:US18563175
申请日:2022-05-26
Inventor: Yan Zhang , Zhonggang Hou , Renke Tan , Ailong Ke
CPC classification number: C12N15/907 , C12N9/22 , C12N15/11 , C12N2310/20 , C12N2800/80
Abstract: The present disclosure provides systems and methods of altering a nucleic acid sequence, which comprise an engineered Type I CRISPR/Cas system comprising Cas3 and Cas11. Particularly. the system and methods described herein use compact engineered CRISPR-Cas3 systems (e.g., Type I-C, Type I-B, or Type I-D) for genetic manipulations (e.g., in eukaryotic cells).
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公开(公告)号:US12031161B2
公开(公告)日:2024-07-09
申请号:US17497367
申请日:2021-10-08
Applicant: University of Massachusetts
Inventor: Michael P. Czech , Emmanouela Tsagkaraki , Sarah M. Nicoloro , Silvia Corvera
CPC classification number: C12N9/22 , A61K35/35 , A61P3/04 , C12N5/0653 , C12N15/11 , C12N15/907 , C12N2310/20 , C12N2506/13 , C12N2510/00 , C12N2800/80
Abstract: Provided herein are methods and compositions for disrupting expression of nuclear receptor interacting protein 1 (Nrip1) in adipose cells, and methods of use of such adipose cells for treating, or reducing risk of, a condition associated with an elevated body mass index (BMI).
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公开(公告)号:US20240218027A1
公开(公告)日:2024-07-04
申请号:US18503341
申请日:2023-11-07
Applicant: THE SCRIPPS RESEARCH INSTITUTE
Inventor: Floyd E. ROMESBERG , Yorke ZHANG
IPC: C07K14/405 , C12N9/22 , C12N15/10 , C12N15/11 , C12N15/90
CPC classification number: C07K14/405 , C12N9/22 , C12N15/10 , C12N15/11 , C12N15/90 , C12N2310/20 , C12N2800/80
Abstract: Disclosed herein are proteins, methods, cells, engineered microorganisms, and kits for generating a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum. Also disclosed herein proteins, methods, cells, engineered microorganisms, and kits for production of a nucleic acid molecule that comprises an unnatural nucleotide utilizing a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum.
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公开(公告)号:US12024728B2
公开(公告)日:2024-07-02
申请号:US18356013
申请日:2023-07-20
Applicant: Flagship Pioneering Innovations VI, LLC
Inventor: Robert Charles Altshuler , Anne Helen Bothmer , Cecilia Giovanna Silvia Cotta-Ramusino , Randi Michelle Kotlar , Ananya Ray , Nathaniel Roquet , Carlos Sanchez , Barrett Ethan Steinberg
CPC classification number: C12N9/22 , C12N9/1276 , C12N15/1082 , C12N15/11 , C12N15/907 , C12Y207/07049 , C12N2310/20 , C12N2800/80
Abstract: The disclosure provides, e.g., compositions, systems, and methods for targeting, editing, modifying, or manipulating a host cell's genome at one or more locations in a DNA sequence in a cell, tissue, or subject.
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公开(公告)号:US20240209367A1
公开(公告)日:2024-06-27
申请号:US18519153
申请日:2023-11-27
Applicant: Complement Therapeutics Limited
Inventor: Simon CLARK , Mustafa Munye , Sonika Rathi
CPC classification number: C12N15/113 , A61K38/465 , C12N9/22 , C12N15/11 , C12N15/907 , G01N33/6851 , G01N33/6893 , C12N2310/11 , C12N2310/14 , C12N2310/141 , C12N2310/20 , C12N2310/531 , C12N2800/80 , G01N2333/4716
Abstract: Agents for reducing the gene and/or protein expression of FH family proteins are disclosed. Also disclosed are articles encoding the agents and methods of using the agents, including in therapeutic and prophylactic methods.
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公开(公告)号:US20240200102A1
公开(公告)日:2024-06-20
申请号:US18541481
申请日:2023-12-15
Applicant: Pairwise Plants Services, Inc.
Inventor: Yongjoo Kim
IPC: C12N15/90 , C07K14/195 , C12N9/12 , C12N9/22 , C12N15/113
CPC classification number: C12N15/90 , C07K14/195 , C12N9/1276 , C12N9/22 , C12N15/113 , C07K2319/92 , C12N2310/20 , C12N2800/80
Abstract: Described herein are fusion proteins that include an intein polypeptide along with methods of using of such proteins. Fusion proteins described herein may include a Cas12a polypeptide and an intein polypeptide or a polypeptide of interest (e.g., a reverse transcriptase polypeptide) and an intein polypeptide. Also described herein are compositions and systems for modifying or editing a target nucleic acid.
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公开(公告)号:US12012458B2
公开(公告)日:2024-06-18
申请号:US17303952
申请日:2021-06-10
Applicant: Nkarta, Inc.
Inventor: James Barnaby Trager , Alexandra Leida Liana Lazetic , Ivan Chan , Chao Guo , Katherine Jamboretz
IPC: C07K16/28 , C07K14/705 , C07K14/715 , C07K14/725 , C12N15/90
CPC classification number: C07K16/2875 , C07K14/7051 , C07K14/70578 , C07K14/7155 , C12N15/907 , C07K2317/622 , C07K2319/02 , C07K2319/03 , C12N2310/20 , C12N2800/80
Abstract: Several embodiments of the methods and compositions disclosed herein relate to immune cells that are engineered to express chimeric antigen receptors (CAR) and/or genetically modified to reduce potential side effects of cellular immunotherapy. Several embodiments relate to genetic modifications to the immune cells, such as Natural Killer (NK) cells, to reduce, substantially, reduce, or eliminate expression of a marker by the immune cells that would otherwise cause them to be self-targeted by the CAR. In several embodiments, the CAR targets CD70, and in some embodiments is used for renal cell carcinoma immunotherapy.