公开(公告)号：US20150027891A1

公开(公告)日：2015-01-29

申请号：US13951881

申请日：2013-07-26

Applicant: General Electric Company

Inventor： Christopher Michael Puleo ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk ,  Nichole Lea Wood ,  Li Zhu ,  Craig Patrick Galligan

IPC: B01D57/02

CPC classification number: B01D57/02 ,  C12N15/101

Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

Abstract translation: 提供了通过电洗脱来从生物样品中洗脱生物分子如核酸的方法。 方法的实例包括各种步骤，例如将生物样品装载到包括壳体的装置中，至少两个可操作地耦合到壳体的导电氧化还原聚合物电极和设置在至少一个电极上的生物分子不可渗透层。 样品的负载之后是引发电连接以产生足以从生物样品中洗脱生物分子的电场强度; 并从生物样品中洗脱生物分子。