公开(公告)号：US20120328240A1

公开(公告)日：2012-12-27

申请号：US13578665

申请日：2011-02-14

Applicant: Changbao Ma ,  Zhaowei Liu

Inventor： Changbao Ma ,  Zhaowei Liu

IPC: G02B3/00 ,  G02B6/32

CPC classification number: G02B6/1226 ,  B82Y20/00 ,  G02B1/002 ,  G02B6/107 ,  G02F2203/10 ,  H01Q15/0086 ,  H01Q15/02

Abstract: Devices based on metamaterial structures to guide and manipulate light, other electromagnetic radiation and acoustic waves. For example, a lens can include a metamaterial structure comprising nano structures of metallic and dielectric materials; and a plasmonic waveguide coupler formed over the metamaterial structure for coupling electromagnetic radiation to or from metamaterial structure. The metamaterial structure has an anisotropic structure and the plasmonic waveguide coupler is structured to include metal and non-metal parts to support surface plasmon polaritons and to cause different phase delays at different locations of an interface with the metamaterial structure in a way that the metamaterial structure and the plasmonic waveguide coupler effect a lens for performing a Fourier transform of the electromagnetic radiation coupled between the metamaterial structure and the plasmonic waveguide coupler.

Abstract translation: 基于超材料结构的设备来引导和操纵光，其他电磁辐射和声波。 例如，透镜可以包括包括金属和介电材料的纳米结构的超材料结构; 以及形成在超材料结构上的等离子体激元波导耦合器，用于将电磁辐射耦合到超材料结构或从超材料结构耦合。 超材料结构具有各向异性结构，等离子体激元波导耦合器被构造为包括金属和非金属部分以支持表面等离子体激元极化并且在与超材料结构的界面的不同位置处引起不同的相位延迟，使得超材料结构 并且等离子体激元波导耦合器影响用于对耦合在超材料结构和等离子体激元波导耦合器之间的电磁辐射进行傅里叶变换的透镜。

公开(公告)号：US07435545B2

公开(公告)日：2008-10-14

申请号：US11103356

申请日：2005-04-11

Applicant: Zhaowei Liu ,  Thomas E. Kane

Inventor： Zhaowei Liu ,  Thomas E. Kane

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q2565/125 ,  C12Q2565/102 ,  C12Q2545/101

Abstract: According to a method of determining a size of a sample polynucleotide, a sample polynucleotide is subjected to electrophoresis in the presence of a fluorescent compound having a first fluorescence spectrum. Detection of light of the first fluorescence spectrum is indicative of the presence of the sample polynucleotide. One or more size standards are also subjected to electrophoresis, optionally in the presence of the sample polynucleotide. If more than one size standard is used, the different size standards typically have different mobilities. The size standards are generally essentially or completely free of polynucleotides. Migration coordinates, e.g., migration times, of the sample polynucleotide and size standard(s) are determined. A size of the sample polynucleotide can be determined using the migration coordinate of the sample polynucleotide and the migration coordinate(s) of the size standard(s).

Abstract translation: 根据测定样品多核苷酸的大小的方法，在具有第一荧光光谱的荧光化合物的存在下对样品多核苷酸进行电泳。 第一荧光光谱的光的检测指示样品多核苷酸的存在。 也可以在样品多核苷酸的存在下，一个或多个尺寸标准品进行电泳。 如果使用多于一个尺寸的标准，则不同尺寸的标准通常具有不同的移动性。 大小标准通常基本上或完全不含多核苷酸。 测定样品多核苷酸的迁移坐标，例如迁移时间和大小标准。 可以使用样品多核苷酸的迁移坐标和尺寸标准的迁移坐标来确定样品多核苷酸的大小。

公开(公告)号：US07282126B2

公开(公告)日：2007-10-16

申请号：US10617750

申请日：2003-07-14

Applicant: Zhaowei Liu ,  Zhiyong Guo ,  Christina Maye ,  Kevin R. Gutshall

Inventor： Zhaowei Liu ,  Zhiyong Guo ,  Christina Maye ,  Kevin R. Gutshall

IPC: G01N27/453

CPC classification number: G01N27/44773 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q2600/156 ,  G01N27/44704 ,  G01N27/44721 ,  C12Q2565/125 ,  C12Q2545/113 ,  C12Q2527/101 ,  C12Q2565/131 ,  C12Q2527/107

Abstract: The present invention relates to a method of determining the genotype of a sample polynucleotide having at least a first variant site. At least a portion of the sample polynucleotide is amplified to obtain first amplicons, the first amplicons including the first variant site. The first amplicons are combined with first and second different polynucleotide controls, the first and second polynucleotide controls differing by at least one base therealong, the position of the at least one differing base corresponding to the first variant site of the sample polynucleotide. A plurality of first duplexes are prepared, each of at least some of the first duplexes comprising (i) a polynucleotide strand of one of the first amplicons and (ii) a complementary polynucleotide strand of the first polynucleotide control. A plurality of second duplexes are prepared, each of at least some of the second duplexes comprising (i) a polynucleotide strand of one of the first amplicons and (ii) a complementary polynucleotide strand of the second polynucleotide control. The first and second duplexes are subjected to temperature gradient electrophoresis (TGE) to obtain first and second electrophoresis data. The genotype of the first variant site of the sample polynucleotide is determiend based on the first and second electrophoresis data.

Abstract translation: 本发明涉及确定具有至少第一变异位点的样品多核苷酸的基因型的方法。 扩增样品多核苷酸的至少一部分以获得第一扩增子，第一扩增子包括第一变体位点。 将第一扩增子与第一和第二不同多核苷酸对照组合，第一和第二多核苷酸对照与其中至少一个碱基不同，所述至少一个不同碱基对应于样品多核苷酸的第一个变异位点的位置。 制备多个第一双链体，至少一些第一双链体包含（i）第一扩增子之一的多核苷酸链和（ii）第一多核苷酸对照的互补多核苷酸链。 制备多个第二双链体，至少一些第二双链体包含（i）第一扩增子之一的多核苷酸链和（ii）第二多核苷酸对照的互补多核苷酸链。 对第一和​​第二双链体进行温度梯度电泳（TGE）以获得第一和第二电泳数据。 基于第一和第二电泳数据确定样品多核苷酸的第一变体位点的基因型。

公开(公告)号：US20050064473A1

公开(公告)日：2005-03-24

申请号：US10910256

申请日：2004-08-02

Applicant: Zhaowei Liu ,  Yiqiong Wu

Inventor： Zhaowei Liu ,  Yiqiong Wu

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/131

Abstract: A method for determining a frequency of single nucleotide polymorphism (SNP) within genomic DNA includes providing genomic DNA of each of a plurality of different organisms. The genomic DNA of each organism includes first and second portions, e.g., first and second strands. First and second amplicons are prepared from the genomic DNA of each organism. The first amplicon corresponds to the first portion of the genomic DNA and the second amplicon corresponds to the second portion of the genomic DNA. A plurality of duplexes is prepared from the first and second amplicons of the genomic DNA of each organism. At least some of the duplexes include a portion of one of the first amplicons and a portion of one of the second amplicons. The duplexes are subjected to temperature gradient electrophoresis to obtain first electrophoresis data indicative of the rate of SNP at a first location in the genomic DNA of the plurality of organisms.

Abstract translation: 用于确定基因组DNA内的单核苷酸多态性（SNP）的频率的方法包括提供多种不同生物体中的每一种的基因组DNA。 每个生物体的基因组DNA包括第一和第二部分，例如第一和第二链。 从每个生物体的基因组DNA制备第一和第二扩增子。 第一扩增子对应于基因组DNA的第一部分，第二扩增子对应于基因组DNA的第二部分。 从每个生物体的基因组DNA的第一和第二扩增子制备多个双链体。 至少一些双链体包括第一扩增子之一和第二扩增子之一的一部分的一部分。 对双链体进行温度梯度电泳以获得指示多个生物体的基因组DNA中第一位置处的SNP的速率的第一电泳数据。

公开(公告)号：US20050064400A1

公开(公告)日：2005-03-24

申请号：US10287808

申请日：2002-11-05

Applicant: Zhiyong Guo ,  Zhaowei Liu ,  Qingbo Li

Inventor： Zhiyong Guo ,  Zhaowei Liu ,  Qingbo Li

IPC: C12Q1/68 ,  C12Q1/6827 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC classification number: C12Q1/6827 ,  C12Q2565/125 ,  C12Q2527/101

Abstract: The present invention relates to a method for determining the presence of a mutation in a first sample comprising first polynucleotides. The reference sample comprises reference polynucleotides. The first sample and a reference sample are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first sample and the reference sample is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference polynucleotides. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference polynucleotides. The data are processed to determine the presence of mutation in the first polynucleotides.

Abstract translation: 本发明涉及一种用于确定包含第一多核苷酸的第一样品中突变存在的方法。 参考样品包括参考多核苷酸。 第一个样品和参考样品在至少一个插层染料存在下进行电泳。 在电泳期间，第一样品和参考样品的温度改变足以改变至少一个第一或参考多核苷酸的电泳迁移率的量。 获得荧光强度数据。 荧光强度数据表明第一和参考多核苷酸的存在。 处理数据以确定第一多核苷酸中突变的存在。