公开(公告)号：US20170081712A1

公开(公告)日：2017-03-23

申请号：US15273200

申请日：2016-09-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shifeng LI ,  Shoulian DONG ,  Theodore E. STRAUB ,  David N. KEYS

IPC: C12Q1/68 ,  G01N27/30 ,  G01N27/414

CPC classification number: C12Q1/6825 ,  C12Q1/6837 ,  C12Q1/6851 ,  G01N27/301 ,  G01N27/4145 ,  C12Q2531/113 ,  C12Q2563/116 ,  C12Q2563/159 ,  C12Q2565/607

Abstract: A system for detecting one or more target molecules includes a reaction device and a temperature controller. The reaction device comprises a plurality of reaction sites and plurality of field effect transistors corresponding to the plurality of reaction sites. At least two of the reaction sites may receive a reaction solution including two target molecules. The temperature controller is configured to control a temperature of at least one of the reaction sites or the reaction solution received by the at least one reaction site. At least some of the reaction sites may receive a reagent supporting an amplification reaction. When the reaction is subjected to an amplification assay, an amplified product is produced in the at least some of the reaction sites. The plurality of field effect transistors are configured to detect at least one of the amplified products.

公开(公告)号：US20160265031A1

公开(公告)日：2016-09-15

申请号：US15066139

申请日：2016-03-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chunmei LIU ,  Shoulian DONG ,  Linda WONG

IPC: C12Q1/68

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

Abstract translation: 本文提供了用于捕获，检测和定量成熟小RNA的方法，组合物和试剂盒。 方法的实施方案包括通过将5'连接衔接子连接到5'端并将3'端聚腺苷酸来拖尾成熟小RNA的5'和3'末端。 其他实施方案包括用通用逆转录引物逆转录连接的多聚腺苷酸成熟小RNA，并用通用引物扩增cDNA。