公开(公告)号：US12152256B2

公开(公告)日：2024-11-26

申请号：US17932844

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Peter Vander Horn ,  Cheng-Yao Chen ,  Guobin Luo ,  Michael Previte ,  Jamshid Temirov ,  Theo Nikiforov ,  Zhaohui Zhou ,  Hongye Sun ,  Yufang Wang ,  Stefanie Yukiko Nishimura ,  Hongyi Wang ,  Marian Peris ,  Barnett Rosenblum ,  Michael Phelan

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.