公开(公告)号：US09164060B2

公开(公告)日：2015-10-20

申请号：US14401399

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Yves LeBlanc

IPC: H01J49/40 ,  G01N33/22 ,  G01N27/62 ,  H01J49/00 ,  H01J49/04

CPC classification number: G01N27/624 ,  H01J49/0031 ,  H01J49/0431

Abstract: Methods and systems for performing mass spectrometry of analytes labeled with isobaric tags are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can enable enhanced discrimination between an analyte of interest and one or more interfering species when using isobaric tagging techniques.

Abstract translation: 本文提供了用等压标签标记的分析物进行质谱分析的方法和系统。 根据申请人的教导的各个方面，当使用等压标签技术时，方法和系统可以增强感兴趣的分析物与一种或多种干扰物质之间的增强的区分。

公开(公告)号：US20150260684A1

公开(公告)日：2015-09-17

申请号：US14431422

申请日：2013-11-16

Applicant: DH Technologies Development Pte. Ltd. ,  UNIVERSITY OF WOLLONGONG

Inventor： Stephen J. Blanksby ,  John Lawrence Campbell ,  Yves LeBlanc ,  Alan T. Maccarone ,  Todd W. Mitchell

IPC: G01N27/62 ,  H01J49/10 ,  H01J49/00

CPC classification number: G01N27/624 ,  G01N27/622 ,  H01J49/0031 ,  H01J49/0077 ,  H01J49/10

Abstract: Methods and systems for performing mass spectrometry are provided herein. The method includes ionizing one or more compounds to generate ions, passing the ions into an ion mobility spectrometer, providing ozone, reacting the ions with ozone to produce ozone-induced fragment ions, and performing mass analysis and detection of the ozone-induced fragment ions. The system can comprise an ion source for ionizing one or more compounds to generate ions, an ion mobility spectrometer for receiving and separating the ions, an ozone supply for introducing ozone into the system; a reaction region for reacting the ions with the ozone to produce ozone-induced fragment ions, and a mass spectrometer for performing mass analysis and detection of the ozone-induced fragment ions.

Abstract translation: 本文提供了用于进行质谱分析的方法和系统。 该方法包括电离一种或多种化合物以产生离子，将离子传递到离子迁移谱仪中，提供臭氧，使离子与臭氧反应产生臭氧诱导的碎片离子，并进行臭氧诱导的碎片离子的质量分析和检测 。 该系统可以包括用于离子化一种或多种化合物以产生离子的离子源，用于接收和分离离子的离子迁移谱仪，用于将臭氧引入系统的臭氧供应; 用于使离子与臭氧反应以产生臭氧诱导的碎片离子的反应区域和用于进行臭氧诱导的碎片离子的质量分析和检测的质谱仪。

公开(公告)号：US11953478B2

公开(公告)日：2024-04-09

申请号：US17593349

申请日：2020-03-10

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Nic G. Bloomfield ,  Lyle Lorrence Burton ,  John Lawrence Campbell ,  David M. Cox ,  Eva Duchoslav ,  Adam San-Ho Lau

IPC: H01J49/00 ,  G01N30/72 ,  G01N30/86 ,  H01J49/04 ,  H01J49/16 ,  H01J49/42 ,  H01J49/44

CPC classification number: G01N30/8631 ,  G01N30/7206 ,  G01N30/7233 ,  H01J49/0031 ,  H01J49/0418 ,  H01J49/167 ,  H01J49/4225 ,  H01J49/446

Abstract: Each of one or more unknown compounds are separated from a sample over a separation time period. Separated compounds are ionized, producing one or more compound precursor ions for each of the unknown compounds and a plurality of background precursor ions. A precursor ion mass spectrum is measured for the combined compound and background precursor ions at each time step of a plurality of time steps spread across the separation time period, producing a plurality of precursor ion mass spectra. One or more background precursor ions are selected from the plurality of precursor ion mass spectra that have a resolving power in a range below a threshold expected resolving power. A separation time is detected for an unknown compound when a decrease in an intensity measurement of the selected background precursor ions over a time period exceeds a threshold decrease in intensity with respect to time.

公开(公告)号：US20240003846A1

公开(公告)日：2024-01-04

申请号：US18036098

申请日：2021-11-09

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Subhasish PURKAYASTHA

IPC: G01N27/624

CPC classification number: G01N27/624

Abstract: Method for separating, detecting, and/or quantifying steroid isomers using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the method can provide for the separation of racemic or non-racemic mixtures of steroid isomers that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), including both steroid stereoisomers and constitutional steroid isomers. The method may further comprise detecting the ionized derivatized steroids transported from the differential mobility spectrometer at a first combination of compensation voltage and separation voltage and at a second combination of compensation voltage and separation voltage applied to the differential mobility spectrometer, wherein the first combination is configured to optimize transmission of a first ionized derivatized steroid corresponding to a first steroid of an isomeric steroid pair and the second combination is configured to optimize transmission of a second ionized derivatized steroid corresponding to a second steroid of the isomeric steroid pair.

公开(公告)号：US20220359179A1

公开(公告)日：2022-11-10

申请号：US17814256

申请日：2022-07-22

Applicant: Scott Hopkins ,  DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell ,  Scott Hopkins

IPC: H01J49/00 ,  G06N20/20 ,  G01N27/62 ,  G01N27/623

Abstract: A plurality of known compounds with known CCS values is analyzed using a DMS device. The DMS device determines how the intensities of their transmitted ions vary with different separation voltages (SVs) and compensation voltages (CVs). A machine learning algorithm builds a data model from the known m/z value, known CCS value, and measured pairs of CV and SV values that provide optimal transmission through the DMS device for each of the known compounds. An unknown compound with an unknown CCS value is then analyzed. The DMS device determines how the intensity of its ions varies with the same different SVs and CVs. Finally, the machine learning algorithm predicts the CCS value of the unknown compound from the data model, the known m/z of the unknown compound, and the measured pairs of CV and SV values that provide optimal transmission through the DMS device for the unknown compound.

公开(公告)号：US20170248548A1

公开(公告)日：2017-08-31

申请号：US15516387

申请日：2015-09-21

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Eva Duchoslav ,  Yves Le Blanc ,  David M. Cox

IPC: G01N27/62

CPC classification number: G01N27/624 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004 ,  H01J49/0045

Abstract: Systems and methods are provided for providing a DMS precursor ion survey scan. An ion source configured to receive a sample is instructed to ionize the sample using a processor. A DMS device configured to receive ions from the ion source is instructed to separate precursor ions received from the ion source and transmit precursor ions using two or more CoVs using the processor. A mass analyzer configured to receive transmitted precursor ions from the DMS device is instructed to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the processor. The measured m/z intensities of the transmitted precursor ions received from the mass analyzer are stored as a function of m/z value and CoV using the processor. This produces a stored two-dimensional mapping of m/z intensities of the precursor ions of the sample.

公开(公告)号：US20150129762A1

公开(公告)日：2015-05-14

申请号：US14401399

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Yves LeBlanc

IPC: G01N27/62 ,  H01J49/00

CPC classification number: G01N27/624 ,  H01J49/0031 ,  H01J49/0431

Abstract: Methods and systems for performing mass spectrometry of analytes labeled with isobaric tags are provided herein. In accordance with various aspects of the applicants' teachings, the methods and systems can enable enhanced discrimination between an analyte of interest and one or more interfering species when using isobaric tagging techniques.

Abstract translation: 本文提供了用等压标签标记的分析物进行质谱分析的方法和系统。 根据申请人的教导的各个方面，当使用等压标签技术时，方法和系统可以增强感兴趣的分析物与一种或多种干扰物质之间的增强的区分。

公开(公告)号：US20150097113A1

公开(公告)日：2015-04-09

申请号：US14401034

申请日：2013-04-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell ,  Stephen A. Tate

IPC: H01J49/00

CPC classification number: H01J49/0081 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/004

Abstract: Systems and methods are used to analyze a sample using variable detection scan resolutions. A tandem mass spectrometer is instructed to perform at least two scans of a sample with different detection scan resolutions using a processor. The tandem mass spectrometer includes a mass analyzer that allows variable detection scan resolutions. The selection of the different detection scan resolutions can be based on one or more properties of sample compounds. The properties may include a sample compound molecular weight distribution that is calculated from a molecular weight distribution of expected compounds or is determined from a list of molecular weights for one or more known compounds. The tandem mass spectrometer can also be instructed to perform an analysis of the sample before instructing the tandem mass spectrometer to perform the at least two scans of the sample.

Abstract translation: 系统和方法用于使用可变检测扫描分辨率分析样本。 指示串联质谱仪使用处理器对具有不同检测扫描分辨率的样品进行至少两次扫描。 串联质谱仪包括质量分析仪，允许可变检测扫描分辨率。 不同检测扫描分辨率的选择可以基于样品化合物的一种或多种性质。 性质可以包括根据预期化合物的分子量分布计算的样品化合物分子量分布，或由一种或多种已知化合物的分子量列表确定。 也可以指示串联质谱仪在指示串联质谱仪进行样品的至少两次扫描之前对样品进行分析。

公开(公告)号：US20140357502A1

公开(公告)日：2014-12-04

申请号：US14365983

申请日：2012-11-28

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD

Inventor： John Lawrence Campbell ,  Yves Le Blanc ,  Alexandre V. Loboda ,  Igor Chernushevich

IPC: G06F19/00

CPC classification number: G06F19/703 ,  H01J49/0031 ,  H01J49/004

Abstract: In a first location of a mass spectrometer, a plurality of ionized molecules of an ion source are selected that have mass-to-charge ratios within a mass-to-charge ratio window width. The plurality of selected ionized molecules are transmitted from a first to a second location. Reagent ions are transmitted to the second location to reduce a charge state of one or more of the plurality of selected ionized molecules. A mass analyzer is used to analyze the plurality of reduced ionized molecules and produce a mass spectrum. A compound is identified from a peak of the spectrum that has a mass-to-charge ratio less than or equal to the highest mass-to-charge ratio in the window width if the noise is multiply charged and greater than the highest mass-to-charge ratio in the window width if the noise is singly charged.

Abstract translation: 在质谱仪的第一位置，选择离子源的多个离子化分子，其具有在质荷比窗口宽度之内的质荷比。 多个选择的电离分子从第一位置传递到第二位置。 试剂离子被传递到第二位置以减少多个选定的电离分子中的一个或多个的电荷状态。 质量分析器用于分析多个还原离子化分子并产生质谱。 如果噪声是多次充电并且大于最高质量 - 到达的质量 - 荷电比，如果光谱的峰值具有小于或等于窗口宽度中的最高质荷比的化合物， 如果噪声被单独充电，则窗口宽度的充电比例。

公开(公告)号：US12196709B2

公开(公告)日：2025-01-14

申请号：US17758646

申请日：2021-01-14

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell ,  Brendon Seale

IPC: G01N27/624 ,  G01N27/623

Abstract: A DMS device receives a curtain gas that includes a chemical modifier into its curtain plate. Before or while receiving ions of an analyte, the DMS device steps the CoV through a series of values in order to apply different CoV values to at least one precursor ion derived from the chemical modifier. For each CoV value of the series of values, a mass spectrometer selects and mass analyzes the at least one precursor ion. An intensity is produced for each CoV value of the series of values. An intensity versus CoV value peak is calculated from the intensities measured. A representative CoV value is calculated for the peak. The difference between the representative CoV value and known CoV values that represent an uncontaminated curtain plate is calculated. If the difference is greater than or equal to a predetermined threshold value, the curtain plate is determined to be contaminated.