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公开(公告)号：US10808217B2
公开(公告)日：2020-10-20
申请号：US15518989
申请日：2015-10-14
Applicant: Becton, Dickinson and Company
Inventor： Patrick Shawn Beaty , Michael A. Brasch , Suneil Hosmane , David Polley , Julie L. Rosales , Kerry Lynn Smith
Abstract: A system and method with increased sensitivity to microorganism growth. The system includes signal processing electronic circuit connected to a consumable or vessel through two or more electrodes that fully penetrate the vessel and are in contact with the fluid contents. The electronic circuit is configured to detect a component of the total impedance of the sample, specifically the “out-of-phase” or imaginary reactance component, which has a sensitive response to organism growth in a frequency-dependent manner. The system detects changes in both the composition of charged molecules in the liquid matrix and the number of microorganisms based on monitoring the sample for change in this parameter. This results in a 5-70% reduction in time-to-detection (TTD). The system and method detect organisms in a plurality of vessel shapes, volumes, and matrix (or media) formats. The electrodes are fully immersed in a continuous body of liquid sample. The distance between electrodes may be adjusted or tuned to fit the needs of the vessel. The voltage inputs can also be adjusted to allow proper detection of the contents within the vessel.
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12.发明授权公开(公告)号：US10519482B2
公开(公告)日：2019-12-31
申请号：US14381388
申请日：2013-02-28
Applicant: Becton, Dickinson and Company
Inventor： Susan M. Kircher , Vanda White , William B. Brasso , Dyan Luper , James Y. Zhou , Julie L. Rosales , Jeffery H. Bruton , John D. Mantlo , Adrien P. Malick , Donald R. Callihan , Ben Turng , Liping Feng , Curtis M. Gosnell , Patrick Shawn Beaty , John P. Douglass
Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
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13.发明申请FORMULATIONS AND PROCESS FOR ISOLATING VIABLE MICROORGANISM FROM POSITIVE BLOOD CULTURES 审中-公开从积极的血液培养中分离出可见的微生物的制剂和方法公开(公告)号：US20150125895A1
公开(公告)日：2015-05-07
申请号：US14381388
申请日：2013-02-28
Applicant: Becton, Dickinson and Company
Inventor： Susan M. Kircher , Vanda White , William B. Brasso , Dyan Luper , James Y. Zhou , Julie L. Rosales , Jeffery H. Bruton , John D. Mantlo , Adrien P. Malick , Donald R. Callihan , Ben Turng , Liping Feng , Curtis M. Gosnell , Patrick Shawn Beaty , John P. Douglass
Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
Abstract translation: 本文公开的各种实施方案提供了用于从阳性血培养样品中快速分离存活的微生物细胞（包括肺炎链球菌）的试剂和方法。 所得到的微生物颗粒可用于鉴定和基于生长的方法如抗微生物敏感性测试。 本文所述的缓冲剂可以含有碱溶液，非离子洗涤剂，硫醇和任选的氯化铵。 所公开的方法提供了一种用于仅从一个样品制备管和离心同时从PBC样品中快速分离和浓缩活的微生物的方法，同时从哺乳动物血细胞中除去可能干扰鉴定方法的细胞碎片。
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