公开(公告)号：US10519482B2

公开(公告)日：2019-12-31

申请号：US14381388

申请日：2013-02-28

Applicant: Becton, Dickinson and Company

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12Q1/24 ,  C12N1/02 ,  C12N1/20 ,  C12N1/04 ,  C12Q1/06

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

公开(公告)号：US20190233873A1

公开(公告)日：2019-08-01

申请号：US16376177

申请日：2019-04-05

Applicant: Becton Dickinson and Company

Inventor： Robert Edward Armstrong ,  John Thulin Page ,  Ben Turng ,  Glen Richard Davis ,  Strett Roger Nicolson ,  Timothy Hansen

IPC: C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  B01L3/02

CPC classification number: C12Q1/04 ,  B01L3/0275 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2400/0475 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/38 ,  G01N1/4005 ,  G01N35/00871

Abstract: An automated system for identifying in a biological sample microorganisms and their antimicrobial susceptibility (AST). The system provided an automated platform for preparing, from a single biological sample, inoculates for both ID and AST. The system loads a plate for ID testing as samples are being prepared for AST testing. The system tracks the sample and the inoculates from the samples to link the test results to the sample and the patients from whom the sample was obtained.

公开(公告)号：US20160040210A1

公开(公告)日：2016-02-11

申请号：US14886250

申请日：2015-10-19

Applicant: Becton, Dickinson and Company

Inventor： Robert Edward Armstrong ,  John Thulin Page ,  Ben Turng ,  Glen Richard Davis ,  Strett Roger Nicolson ,  Timothy Hansen

IPC: C12Q1/04 ,  C12Q1/24 ,  C12Q1/18

CPC classification number: C12Q1/04 ,  B01L3/0275 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2400/0475 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/38 ,  G01N1/4005 ,  G01N35/00871

Abstract: An automated system for identifying in a biological sample microorganisms and their antimicrobial susceptibility (AST). The system provided an automated platform for preparing, from a single biological sample, inoculates for both ID and AST. The system loads a plate for ID testing as samples are being prepared for AST testing. The system tracks the sample and the inoculates from the samples to link the test results to the sample and the patients from whom the sample was obtained.

Abstract translation: 用于在生物样品中识别微生物及其抗微生物敏感性（AST）的自动化系统。 该系统提供了一个自动化平台，用于从单个生物样品制备ID和AST的接种。 在为AST测试准备样品时，系统加载了ID测试板。 系统跟踪样品和样品的接种，将测试结果与样品和获得样品的患者连接。

公开(公告)号：US20150125895A1

公开(公告)日：2015-05-07

申请号：US14381388

申请日：2013-02-28

Applicant: Becton, Dickinson and Company

Inventor： Susan M. Kircher ,  Vanda White ,  William B. Brasso ,  Dyan Luper ,  James Y. Zhou ,  Julie L. Rosales ,  Jeffery H. Bruton ,  John D. Mantlo ,  Adrien P. Malick ,  Donald R. Callihan ,  Ben Turng ,  Liping Feng ,  Curtis M. Gosnell ,  Patrick Shawn Beaty ,  John P. Douglass

IPC: C12Q1/14 ,  C12N1/20 ,  C12Q1/24

CPC classification number: C12Q1/14 ,  C12N1/02 ,  C12N1/20 ,  C12Q1/24

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Abstract translation: 本文公开的各种实施方案提供了用于从阳性血培养样品中快速分离存活的微生物细胞（包括肺炎链球菌）的试剂和方法。 所得到的微生物颗粒可用于鉴定和基于生长的方法如抗微生物敏感性测试。 本文所述的缓冲剂可以含有碱溶液，非离子洗涤剂，硫醇和任选的氯化铵。 所公开的方法提供了一种用于仅从一个样品制备管和离心同时从PBC样品中快速分离和浓缩活的微生物的方法，同时从哺乳动物血细胞中除去可能干扰鉴定方法的细胞碎片。

公开(公告)号：US08603769B2

公开(公告)日：2013-12-10

申请号：US13647072

申请日：2012-10-08

Applicant: Becton, Dickinson and Company

Inventor： Liping Feng ,  William B. Brasso ,  Song Shi ,  Ben Turng ,  Susan M. Kircher ,  Vanda White ,  Dyan Luper ,  Julie Rosales ,  Gretta Campbell ,  Adrien Malick

IPC: C12Q1/04 ,  C12Q1/24

CPC classification number: C12Q1/24

Abstract: Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture (“PBC”) samples for use in downstream analyses such as identification and antimicrobial susceptibility testing (“AST”). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of S. pneumoniae.

Abstract translation: 本发明的方法包括从用于下游分析如鉴定和抗微生物敏感性测试（“AST”）的阳性血培养（“PBC”）样品中分离完整的活的微生物。 所述方法包括收集一部分PBC样品，加入含胆碱的溶液，裂解血细胞，分离活微生物，以及进行分离的活的微生物的下游分析。 该方法可以应用于各种革兰氏阳性细菌，革兰氏阴性细菌和/或酵母，特别是肺炎链球菌菌株。