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公开(公告)号：US07081361B2
公开(公告)日：2006-07-25
申请号：US10636339
申请日：2003-08-07
Applicant: Robert Clarence Pearce, III , Dale V. Kiplinger , Jose E. Evaro , Judith Gayle Pruitt , Joseph Thomas Colarusso
Inventor： Robert Clarence Pearce, III , Dale V. Kiplinger , Jose E. Evaro , Judith Gayle Pruitt , Joseph Thomas Colarusso
IPC: C12M1/36
CPC classification number: C12M41/00 , C12M23/42 , C12M41/12 , Y10S435/813
Abstract: A biomass generator useful for continuously growing and withdrawing bacteria to be used in a desired beneficial application, the generator having a bacteria growth chamber; water and nutrient inlet ports; upper and lower outlet ports; a recirculating pump withdrawing and reintroducing fluid to establish a vortex in the growth chamber while controlling foaming and pump cavitation; a low pressure air inlet line discharging air inside the growth chamber above the vortex; a fluid discharge line receiving bacteria-containing fluid from the upper outlet port of the growth chamber; a flush line discharging wash water into the fluid discharge line; and an electrical controller cooperating with solenoid-operated valves and a feeder mechanism to periodically introduce water and nutrients into the chamber, thereby simultaneously causing bacteria-containing fluid to be discharged from the growth chamber through the upper outlet port. A method for growing bacteria using the subject apparatus is also disclosed.
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92.发明申请Device and method for parallel, automated cultivation of cells in technical conditions 审中-公开在技术条件下平行，自动培养细胞的装置和方法
公开(公告)号：US20060141614A1
公开(公告)日：2006-06-29
申请号：US10540299
申请日：2003-12-22
Applicant: Robert Puskeiler , Dirk Weuster-Botz , Karl-Heinz Zacher
Inventor： Robert Puskeiler , Dirk Weuster-Botz , Karl-Heinz Zacher
IPC: C12M1/02
CPC classification number: C12M27/20 , B01F3/04531 , B01F3/04539 , B01F13/065 , B01F13/0818 , B01F13/0827 , B01F15/00071 , B01F15/0022 , B01F15/00915 , B01F15/065 , B01F2003/04567 , B01F2003/04645 , B01F2215/0037 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481 , C12M23/42 , C12M27/02 , C12M41/00 , C12M41/48
Abstract: The invention relates to a device for the cultivation of cells in liquid columns measured on a millimetre scale. Said device comprises a container for receiving a liquid culture suspension of the cells, a mixing element for mixing the culture suspension in the container around a mixing axis. The container and/or the mixing element are configured in such a manner that the speed of the flow is modified either locally and/or temporally along a streamline or flow line around the mixing axis.
Abstract translation: 本发明涉及一种用于培养在毫米级测量的液体柱中的细胞的装置。 所述装置包括用于接收细胞的液体培养悬浮液的容器，用于混合容器中的培养悬浮液围绕混合轴线的混合元件。 容器和/或混合元件被配置成使得流动的速度在混合轴线周围的流线或流动线路局部地和/或时间上被修改。
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公开(公告)号：US20060099705A1
公开(公告)日：2006-05-11
申请号：US10525538
申请日：2003-08-27
Applicant: John Wikswo , David Cliffel , Eugene Leboeuf , Randall Reiserer
Inventor： John Wikswo , David Cliffel , Eugene Leboeuf , Randall Reiserer
IPC: C12M1/34
CPC classification number: C12M23/16 , B01L3/5027 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/163 , B01L2400/0472 , C12M23/12 , C12M29/10 , C12M41/00 , Y10S435/808
Abstract: A bioreactor (200) for cultivating living cells in a liquid medium. In one embodiment, the bioreactor (200) has a substrate (230) having a first surface and an opposite second surface, defining a channel (202) therein, and a plurality of chambers (204) formed in the substrate, wherein each of the plurality of chambers (204) is adapted for receiving cells in a liquid medium and formed with an open end (262), an opposite closed end (264) and side walls, the open end (262) and the closed end (264) defining a depth, d, therebetween for the corresponding chamber (206), the sidewalls defining a width, w, therebetween for the corresponding chamber (206). As such formed, each chamber (206) is in fluid communication with the channel (202) through the open end of the chamber (206), and at least two of the plurality of chambers (206) have depths or widths the same or different form each other.
Abstract translation: 一种用于在液体培养基中培养活细胞的生物反应器（200）。 在一个实施例中，生物反应器（200）具有衬底（230），其具有在其中限定通道（202）的第一表面和相对的第二表面以及形成在衬底中的多个腔室（204），其中 多个腔室（204）适于在液体介质中接收细胞并形成有开口端（262），相对的封闭端（264）和侧壁，开口端（262）和闭合端（264）限定 其间的深度d用于相应的腔室（206），所述侧壁在其间为相应的腔室（206）限定宽度w。 这样形成，每个室（206）通过腔室（206）的开口端与通道（202）流体连通，并且多个腔室（206）中的至少两个具有相同或不同的深度或宽度 形成对方
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公开(公告)号：US20030034300A1
公开(公告)日：2003-02-20
申请号：US09919710
申请日：2001-08-01
Inventor： Vadake R. Srinivasan , John J. Sansalone
IPC: C02F003/28
Abstract: A simple, reliable, inexpensive, and efficient anaerobic digester for treating organic wastes at a shortened residence time is described. The anaerobic digester is a multi-chambered digester that can handle wastewater and sludge in a large volume at a high flow rate. The digester also allows collection of methane gas for use as an energy source. The reactor is based on a sequential series of reaction chambers in a design that does not require internal moving parts. The volume of the chambers is adjusted to control the relative residence time of the waste to select an anaerobic microorganism group or groups that can efficiently digest the waste presented to that chamber. Under most conditions, no addition of bacteria is necessary. The digester works efficiently using microbes native to the waste material. After the reaction chambers and just prior to leaving as effluent, a settling chamber is located to reclaim the microbes and remove additional solids. In one embodiment, the reactor comprises four sequential chambers. However, other chamber numbers and geometries will achieve the same result if the residence time in each chamber is properly adjusted. Neither pH nor temperature needed to be controlled. However, for a higher yield of methane, pH may be controlled from about 6 to about 8.
Abstract translation: 描述了一种用于在缩短停留时间处理有机废物的简单，可靠，廉价且高效的厌氧消化器。 厌氧消化器是一种多室式蒸煮器，可以高流量大量处理废水和污泥。 蒸煮器还可以收集用作能源的甲烷气体。 反应器基于不需要内部移动部件的设计中的连续的反应室系列。 调整室的体积以控制废物的相对停留时间，以选择能有效消化呈现给该室的废物的厌氧微生物组或组。 在大多数条件下，不需要添加细菌。 消化器可以有效地利用废物原料的微生物。 在反应室之后并且在作为流出物离开之前，定位沉淀室以回收微生物并除去另外的固体。 在一个实施例中，反应器包括四个顺序室。 然而，如果适当地调节每个室中的停留时间，其他室号和几何结果将获得相同的结果。 pH和温度都不需要控制。 然而，对于更高的甲烷产率，可以将pH控制在约6至约8。
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公开(公告)号：US20020117443A1
公开(公告)日：2002-08-29
申请号：US10082515
申请日：2002-02-25
Inventor： Laura Jane Bailey , John Timothy Bailey
IPC: C02F003/32
CPC classification number: C05F17/0018 , C02F1/444 , C02F3/327 , C02F11/04 , C02F2209/40 , C02F2301/103 , C05F3/00 , C12M21/04 , C12M27/06 , C12M41/00 , C12M43/00 , C12M47/02 , Y02A40/205 , Y02E50/343 , Y02P20/145 , Y02W10/18 , Y02W10/23 , Y02W30/43 , Y02W30/47
Abstract: Sealed Tight is an integrated anaerobic digester and aquaculture filtering system for agricultural waste treatment. This systems retrofits existing waste containment facilities, resulting in an economical system for complete waste treatment that, due to its sealed design, protects the environment from contamination, even during flood situations. Thoroughly digested manure as created in this system is a superior, low-odor fertilizer. Aquatic plants used to filter overflow are excellent livestock feed; and stored biogas provides farms with a high level of energetic self-sufficiency.
Abstract translation: 密封紧密是一种综合的厌氧消化器和用于农业废物处理的水产养殖过滤系统。 该系统改造现有的垃圾遏制设施，从而形成一个经济的完整废物处理系统，由于其密封设计，即使在洪水情况下也能保护环境免受污染。 在该系统中产生的彻底消化的肥料是优良的低气味肥料。 用于过滤溢流的水生植物是优良的家畜饲料; 并且储存的沼气为农场提供了高水平的自给自足。
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公开(公告)号：US06214617B1
公开(公告)日：2001-04-10
申请号：US09224645
申请日：1998-12-31
Applicant: Heath H. Herman
Inventor： Heath H. Herman
IPC: C12N500
Abstract: The present invention comprises a novel culture methods and devices in which living cells or subcellular biocatalysts are immobilized by the opposition of forces. The immobilized cells or biocatalysts may be attached to support complexes that add to the resultant vector forces.
Abstract translation: 本发明包括一种新的培养方法和装置，其中活细胞或亚细胞生物催化剂被力的固定化。 固定的细胞或生物催化剂可以连接到支持复合物，这增加了合成的载体力。
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97.发明授权
公开(公告)号：US5994129A
公开(公告)日：1999-11-30
申请号：US889848
申请日：1997-07-08
Applicant: R. Douglas Armstrong , James Maluta , David W. Roecker
Inventor： R. Douglas Armstrong , James Maluta , David W. Roecker
Abstract: A portable cassette is disclosed, for use in receiving, maintaining and growing biological cells ex vivo without exposing the cells to the external environment. The portable cassette is used in combination with a processor instrument that facilitates an initial inoculation of the cassette with cells of the kind to be grown and to distribute those cells in a predetermined pattern (e.g., uniformly) throughout a cell growth chamber. Thereafter, the portable cassette is used in combination with an incubator instrument that incubates the cell growth chamber so that the cells are optimally expanded. The same processor instrument then is used to harvest the expanded cells from the portable cassette. Both instruments are configured to condition the portable cassette during stages of the cell growth process, without disturbing the cassette's sterile system.
Abstract translation: 公开了便携式盒式磁带，用于离子体接收，维持和生长生物细胞而不将细胞暴露于外部环境。 便携式盒与处理器仪器组合使用，其便于初始接种具有待种植种类细胞的盒，并将细胞以预定图案（例如，均匀地）分布在整个细胞生长室中。 此后，便携式盒与孵化器一起使用，该培养器将培养细胞生长室，使得细胞被最佳地扩大。 然后使用相同的处理器乐器从便携式盒式磁带收获扩展的单元。 两个仪器被配置为在细胞生长过程的阶段期间调节便携式盒，而不会干扰盒的无菌系统。
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公开(公告)号：US5882849A
公开(公告)日：1999-03-16
申请号：US673063
申请日：1996-07-01
Applicant: Alexander B. P. Leonard , Mark E. Huntley , Pearn P. Niiler , Donald Redalje
Inventor： Alexander B. P. Leonard , Mark E. Huntley , Pearn P. Niiler , Donald Redalje
CPC classification number: C12N1/12 , C12M21/02 , C12M23/36 , C12M41/00 , C12M41/06 , C12M41/12 , C12M41/26 , C12P23/00
Abstract: A control method for operating aqueous Haematococcus spp., such as H. pluvialis, microorganism growth processes is disclosed which can maintain viable growth conditions of this microorganism which have heretofore not been easily reproduced in commercially valuable quantities. The primary control parameters are the degree of turbulence in the aqueous growth medium and the scale of the apparatus relative to the scale of the turbulent eddies in vessels which are partially filled with the aqueous medium directly affect conditions which are required for optimum growth: light exposure, nutrient supply, sedimentation rate, bulk temperature, gas exchange rate and cell integrity. These control elements can be cast in terms of the Reynolds number (N.sub.re), pH, temperature, amount of impinging light, and NO.sub.2 concentration, depending upon the operative chlorophyll growth stages of Haematococcus spp. and its photoadaptive stages of producing astaxanthin.
Abstract translation: 公开了一种用于操作水溶性红潮球菌属例如H.luluulisis的微生物生长方法的控制方法，其可保持迄今为止不易以商业上有价值重现的该微生物的生长生长条件。 主要控制参数是水性生长培养基中的湍流程度，并且装置的规模相对于部分填充有水介质的容器中的湍流涡流的比例直接影响最佳生长所需的条件：光照 营养供应，沉淀速率，体温，气体交换率和细胞完整性。 这些控制元素可以根据雷诺数（Nre），pH值，温度，入射光量和NO2浓度来进行投射，这取决于流感嗜血杆菌属的操作叶绿素生长阶段。 及其摄影适应期生产虾青素。
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公开(公告)号：US5081035A
公开(公告)日：1992-01-14
申请号：US654418
申请日：1991-02-11
Applicant: Craig R. Halberstadt , A. Rees Midgley
Inventor： Craig R. Halberstadt , A. Rees Midgley
IPC: C12M3/06
CPC classification number: C12M29/10 , C12M23/24 , C12M29/12 , C12M35/04 , C12M35/08 , C12M41/00 , C12M41/32
Abstract: A system for continuous perfusion culturing of anchorage-dependent and anchorage-independent mammalian cell lines, inter alia, wherein in vivo capillary bed conditions are simulated by providing a bioreactor having a growth chamber wherein the cells are grown between a multitubular array comprising at least three functionally separate, inert non-degradable and structurally strong tubes or sets of tubes. A constant nutrient gradient is maintained along the entire length of the tubes by perfusing medium through the tubes at a flow rate which is sufficient to expose all areas of the chamber to fresh medium by convective forces rather than diffusion. Oxygen transfer and removal of toxic wastes is improved by the invention herein. In a preferred embodiment, porous inert expanded Teflon tubes are employed having an inner diameter of approximately between 1 and 2 millimeters.
Abstract translation: 一种用于连续灌注培养锚定依赖和不依赖锚定的哺乳动物细胞系的系统，其中特别地，其中通过提供具有生长室的生物反应器来模拟体内毛细管床条件，其中所述细胞在包含至少三个 功能分离，惰性不可降解和结构强的管或套管。 通过使培养基通过管子以足够的流量使培养基通过对流力而不是扩散将培养室的所有区域暴露于新鲜培养基的流速，保持恒定的养分梯度。 本发明改善了有毒废物的氧气转移和去除。 在一个优选的实施方式中，使用内径大约在1和2毫米之间的多孔惰性膨胀的特氟隆管。
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公开(公告)号：US4530907A
公开(公告)日：1985-07-23
申请号：US448996
申请日：1982-11-10
Applicant: Anders Peterson , Erik Walum
Inventor： Anders Peterson , Erik Walum
CPC classification number: G01N33/5091 , C12M23/10 , C12M23/22 , C12M29/10 , C12M41/00 , C12M41/34 , G01N33/5014
Abstract: The invention aims at replacing animal experiments for determining acute toxicity (LD.sub.50 values) by utilizing stable perfusion-cultivated cell cultures and also at producing a cultivation chamber which is movable during operation, for determining various parameters.
Abstract translation: PCT No.PCT / SE82 / 00050 Sec。 371日期：1982年11月10日 102（e）1982年11月10日日期PCT提交1982年2月19日PCT公布。 出版物WO82 / 03227 日本1982年9月30日。本发明旨在通过利用稳定的灌注培养细胞培养物替代动物实验来确定急性毒性（LD50值），并且还可以在生产操作期间可移动的培养室，以确定各种参数。
