公开(公告)号：US20130292559A1

公开(公告)日：2013-11-07

申请号：US13853958

申请日：2013-03-29

Applicant: Life Technologies Corporation

Inventor： Stephen J. Gunstream ,  Mark F. Oldham

IPC: G01J1/58

CPC classification number: G01J1/58 ,  G01N21/278 ,  G01N21/6452 ,  Y10T436/10 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/113332

Abstract: Implementations of the present invention describe an apparatus for generating calibration factors for a spectral detector instrument. The calibration factors are derived from a calibration plate containing one or more spectral species in each well of the calibration plate. Each well is then exposed to an excitation source that causes the one or more spectral species in each of the wells to fluoresce. The signal response is measured and associated with each spectral species at each different well position in the calibration plate. Next, the measured signal response from each spectral species at each well position in the calibration plate is compared with a predetermined signal response for each spectral species. The results of this comparison can be used to determine a calibration factor for each well and spectral species to compensate for the difference between the measured signal response and the predetermined signal response.

Abstract translation: 本发明的实施方式描述了一种用于产生光谱检测器仪器的校准因子的装置。 校准因子来源于校准板的每个孔中含有一个或多个光谱种​​类的校准板。 然后将每个孔暴露于激发源，使得每个孔中的一个或多个光谱物质发荧光。 测量信号响应并与校准板中每个不同孔位置处的每个光谱种类相关联。 接下来，将来自校准板中每个孔位置处的每个光谱种类的测量信号响应与每个光谱种类的预定信号响应进行比较。 该比较的结果可用于确定每个孔和光谱种类的校准因子，以补偿测量的信号响应与预定信号响应之间的差异。