公开(公告)号：US11773426B2

公开(公告)日：2023-10-03

申请号：US16514814

申请日：2019-07-17

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： David Joun ,  Chieh-Yuan Li ,  Brian Reed ,  Craig Obergfell ,  Devin Dressman ,  Abraham Rosenbaum ,  Scott Benson ,  Andi Broka ,  Srinka Ghosh

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844

CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q1/6844 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537

摘要： In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.