公开(公告)号：US09567578B1

公开(公告)日：2017-02-14

申请号：US15138165

申请日：2016-04-25

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Gyun-Min Lee ,  Da Young Yu ,  Soo Min Noh

IPC: C12N15/63 ,  C12N9/00 ,  C12N5/071 ,  C12N15/85

CPC classification number: C12N9/93 ,  C12N5/0686 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2511/00 ,  C12Y603/01002

Abstract: The present invention relates to a novel GS (glutamine synthetase) gene knock out transgenic HEK293 (human embryonic kidney 293) cell line and a production method of a target protein using the said transgenic HEK293 cell line. Particularly, the present inventors eliminated the expression of GS in the HEK293 cells in order to overcome a barrier of the cell line selection caused by the over-expression of GS, for producing a target protein by GS/MSX system, by which the efficiency of the cell line selection for the high production of a target protein would be increased and accordingly the protein production by the selected cell line would be increased, suggesting that the human originated transgenic HEK293 cell line could be efficiently used for the production of a target protein.

Abstract translation: 本发明涉及使用所述转基因HEK293细胞系敲除转基因HEK293（人胚肾293）细胞系的新型GS（谷氨酰胺合成酶）基因和靶蛋白的制备方法。 特别地，本发明人在HEK293细胞中消除了GS的表达，以克服GS过表达导致的细胞系选择的障碍，用于通过GS / MSX系统产生靶蛋白， 靶蛋白高产量的细胞系选择将增加，因此选择的细胞系的蛋白质产生将增加，表明人源的转基因HEK293细胞系可有效地用于产生靶蛋白。