公开(公告)号：US20200299702A1

公开(公告)日：2020-09-24

申请号：US16811024

申请日：2020-03-06

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jung Hoon AHN ,  Hyunjong BYUN ,  Jiyeon PARK

IPC: C12N15/70 ,  C12N15/78 ,  C07K14/21 ,  C12N9/20

Abstract: The present invention provides a method for effective extracellular secretion of a target protein, by preparing a fusion protein connecting LARDS to the target protein and having pI lowered by adjusting the overall charge of target protein, and by using ABC transporter of a bacterial type 1 secretion system (T1SS). The method can allows a protein be produced at a large amount simply and effectively without a separate purification process.