A method and system for using a positioning reference signal (PRS) of a cell to indicate an operational state of the cell. The cell could have two or more candidate PRS configurations defining physical configurations of its PRS, such as frequency positions of air interface resources that carry the cell's PRS. One such PRS configuration could correspond with one operational state of the cell (e.g., heavy cell load), and another such PRS configuration could correspond with a different operational state of the cell (e.g., normal or light cell load). A base station could thus select and apply a particular PRS configuration to indicate operational state of the cell. And an entity such as a wireless client device or neighboring base station could determine the PRS configuration used in the cell as a way to determine the operational state of the cell.