The present disclosure relates to a two-dimensional fiber array structure including a base which includes a baseboard, a cover board and a spacer layer, and an optical fiber cable is positioned between the baseboard and the cover board, positioning fibers are positioned at two external sides of the optical fiber cable, the spacer layer is abutted with two adjacent fiber layers of the optical fiber cable to reduce the position tolerance along X axis for further improving accuracy, whereby ensuring quality and stability of transmitting optical signal.