A robot cleaner includes a cleaner body for forming appearance of the robot cleaner, a driving unit mounted to the cleaner body and configured to generate a suction force, a suction unit provided at the cleaner body and configured to suck dust-contained air by the driving unit, a first guiding member and a second guiding member communicated with the suction unit, respectively, and spaced from each other, and a cyclone unit configured to filter dust from air sucked through the suction unit using a centrifugal force. The cyclone unit has a first suction opening and a second suction opening communicated with the first guiding member and the second guiding member, respectively. The cyclone unit further has a first cyclone and a second cyclone configured to pass dust-filtered air therethrough.