In various embodiments, a surgical cassette, configured to engage peristaltic pump rollers, may include two or more pump segments between a sheet and a substrate coupled to the sheet. The two or more pump segments on the cassette may produce additional flow (e.g., approximately twice the flow for two segments as opposed to one) than if the cassette had only one pump segment engaging the roller. Further, in some embodiments, the two or more pump segments and rollers on the roller head may be configured to provide a flow profile in which a peak of a pulse from a first pump segment is at least partially out of phase with a peak of a pulse from the second pump segment. The combined resultant flow may then have a flow profile with pulsation amplitudes that are smaller than the individual pump segment pulsation amplitudes.