发明授权
US09388389B2 JFH-1 based HCV cell culture systems for NS5A of genotypes 1-7
有权
基于JFH-1的HCV细胞培养系统NS5A基因型1-7
- 专利标题： JFH-1 based HCV cell culture systems for NS5A of genotypes 1-7
- 专利标题（中）： 基于JFH-1的HCV细胞培养系统NS5A基因型1-7
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申请号： US13499788申请日： 2010-10-01
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公开(公告)号： US09388389B2公开(公告)日： 2016-07-12
- 发明人: Troels Kasper Høyer Scheel , Judith M. Gottwein , Tanja Bertelsen Jensen , Jens Bukh
- 申请人： Troels Kasper Høyer Scheel , Judith M. Gottwein , Tanja Bertelsen Jensen , Jens Bukh
- 申请人地址： DK Hvidovre DK Copenhagen K
- 专利权人： Hvidovre Hospital,Kobenhavns Universitet
- 当前专利权人： Hvidovre Hospital,Kobenhavns Universitet
- 当前专利权人地址： DK Hvidovre DK Copenhagen K
- 代理机构： Thompson Coburn LLP
- 代理商 Charles P. Romano
- 优先权： DK200970142 20091002
- 国际申请： PCT/IB2010/002620 WO 20101001
- 国际公布： WO2011/039639 WO 20110407
- 主分类号： C12N7/00
- IPC分类号： C12N7/00 ; C07K14/005 ; C12Q1/18 ; G01N33/569 ; A61K39/00
摘要：
The present inventors developed hepatitis C virus recombinants expressing NS5A from genotype 1a, 1b, 2a, 3a, 4a, 5a, 6a or 7a in the context of a genotype 2a backbone. Additional recombinants express NS5A and the structural proteins (Core, E1 and E2), p7 and NS2 from genotype 1a, 1b, 3a, 4a, 5a, 6a or 7a in the genotype 2a backbone. Sequence analysis of the recombinants recovered after viral passage in Huh7.5 cells revealed adaptive mutations in NS5A and/or NS3. The importance of these mutations for improved growth kinetics was shown in reverse genetic studies.
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