A barcode detecting and marking device can include a base, a transmission mechanism mounted on the base, a marking mechanism mounted on the base, a code reader adjacent to the transmission mechanism, and a controlling platform adjacent to the base. The transmission mechanism can include a supporting assembly mounted on the base, and a transmission assembly coupled to the supporting assembly. The transmission assembly can include a feeding tray coupled to the supporting assembly, a rolling tray coupler to the supporting assembly, and a first driver coupled to the supporting assembly and the rolling tray. The first driver rotates the rolling tray. The marking mechanism can include a driving member mounted on the base, and an execution member. The controlling platform can be electrically coupled to the transmission mechanism and the code reader.