A double-layered transparent conductive film includes: a first substrate; a first imprint adhesive layer formed on the first substrate, the first imprint adhesive layer defining a first mesh-shaped groove, the first mesh-shaped groove forming a first mesh; a first conductive layer including conductive material filled in the first mesh-shaped groove; a tackifier layer formed on the first imprint adhesive layer and the first conductive layer; a second substrate formed on the tackifier layer; a second imprint adhesive layer formed on the second substrate, the second imprint adhesive layer defining a second mesh-shaped groove, the second mesh-shaped groove forming a second mesh, wherein one of the first mesh and the second mesh is a regular mesh, the other is a random mesh; and a second conductive layer including conductive material filled in the second mesh-shaped groove. During the lamination, no alignment accuracy is needed, such that the production efficiency is greatly improved. A method of manufacturing the double-layered transparent conductive film is also provided.